KARAKTERISTIKE MAGAREĆEG I KOBILJEG MLIJEKA TE NJEGOVO POTENCIJALNO TERAPEUTSKO DJELOVANJE NA HUMANO ZDRAVLJE

Od davnina je upotreba mlijeka kopitara poznata u svijetu. U našim krajevima, u mediteranskom i priobalnom području magarac je uglavnom korišten kao radna životinja, a mlijeko magaraca se koristilo u liječenju bolesti dišnog sustava. U Republici Hrvatskoj definirane su tri pasmine magaraca: istarski magarac, sjevernojadranski magarac i primorsko-dinarski magarac, koji su na popisu ugroženih vrsta. U RH nastoji se popularizirati istarski magarac koji je uključen u uzgojni program, a može proizvesti oko 0,74 l/mužnji mlijeka te primorsko dinarski magarac sa dnevnom proizvodnjom 0,3-0,5 l/mužnji. Laktacija mlijeka uglavnom je pasminska odlika, a na nju također utječe adekvatna prehrana te pravilan postupak sa životinjom. Posljednjih desetak godina provodi se sve više studija po pitanju utjecaja mlijeka kopitara na zdravlje čovjeka. Kod kopitara nije provedena selekcija na mliječnost te je proizvodnja u odnosu na proizvodnju kravljeg mlijeka malena. Mlijeko kopitara je svojim kemijskim sastavom najsličnije humanom mlijeku te zbog toga povoljno utječe na metabolizam, rast i razvoj djece. Kumis, fermentirani proizvod mlijeka kopitara koristi se u liječenju AIDS-a i tuberkuloze. Sa velikom učinkovitošću mlijeko kopitara primjenjuje se kao terapija kod raznih upalnih procesa u organizmu i kod alergije na kravlje mlijeko. Magareće mlijeko se osim za konzumaciju u svježem stanju sve više koristi kao sirovina u kozmetičkoj i farmaceutskoj industriji

The concentrations of Fe, Cu and Zn in selected wines from South-East Serbia

Fruits and vegetables constitute the cheapest source of essential trace elements for the majority of people living in developing countries. The Cu, Fe and Zn contents in twenty selected wine samples produced in the South-East region of Serbia were determined by flame atomic absorption spectrometry. The Cu concentrations varied from 0.07 to 0.57 ppm in wines, and the Fe concentrations fluctuated from 2.93 to 36.2 ppm, while the Zn levels were in the range from 0.21 to 0.67 ppm. The established contents of Cu and Zn showed that wines from this part of the world could serve as good dietary sources of the essential trace metals, and the determined values were within the allowed me¬tals levels in wines for human consumption

Current Issues on Immunotherapy in Children

Therapy of allergic diseases in children implicates avoidance of allergens, standard pharmacotherapy, and immunotherapy. Immunotherapy is the only treatment for allergic diseases with the ability to change the natural course of the disease, thus stopping its further progression as well as the development of new allergic diseases and new sensibilizations. The objective of this chapter is to give insight into the latest data on immunotherapy in treating children with allergic diseases. Methods: The study involved a search for relevant articles on the MEDLINE and PubMed up to 2017. Results: Numerous studies have shown that the sublingual application of allergen-specific immunotherapy is adequate, safe, and efficient in the therapy of immunoglobulin E (IgE)-mediated allergic diseases of the respiratory tract in children, but there are still some questions to be solved concerning the usage of SLIT in children younger than 5 years old, SLIT for polysensitized patients, duration of SLIT, long-lasting effects of SLIT. Conclusions: In order to improve the clinical efficacy of SLIT, we are looking for new routes of administration, new allergens, new protocols as well as combination of SLIT with other immune modulatory treatments

Ecophysiological and biochemical traits of three herbaceous plants growing on the disposed coal combustion fly ash of different weathering stage

The ecophysiological and biochemical traits of Calamagrostis epigejos (Roth.) Festuca rubra L. and Oenothera biennis L. growing on two fly ash lagoons of different weathering stage (L1-3 years and L2-11 years) of the “Nikola Tesla- A” thermoelectric plant (Obrenovac, Serbia) were studied. Species-dependent variations were observed at the L1 lagoon; the greatest vitality (Fv/Fm and Fm/Fo) followed by higher photopigment and total phenolic contents were measured in O. biennis in relation to C. epigejos (p<0.001) and F. rubra (p<0.001). At the L2 site, higher vitality was found in O. biennis (p<0.001) and F. rubra (p<0.01) compared to C. epigejos. O. biennis had the highest photosynthetic capacity. The results obtained in this study indicate that all examined species maintained a level of photosynthesis that allowed them to survive and grow under the stressful conditions in ash lagoons, albeit with lower than optimal success. [Projekat Ministarstva nauke Republike Srbije, br. 173018

Два нова јатрофанска дитерпена из корена Euphorbia nicaeensis

In the previous study fifteen jatrophane diterpenes were isolated from the Euphorbia nicaeensis latex. Fourteen of them have been shown to be potent P-glycoprotein (P-gp) inhibitor in two MDR cancer cells (NCI-H460/R and DLD1-TxR). The aim of this study was to determine whether and which jatro­phane diterpenes can be isolated from the root of the plant, and then to examine their inhibition power on P-glycoprotein of selected cancer cell lines (NCI-H460, DLD1, U87, NCI-H460/R, DLD1-TxR and U87-TxR). Two previously undes­cribed jatrophane diterpenes were isolated from the root of E. nicaeensis col­lected in Deliblato Sand (Serbia). The structures of the isolated compounds were determined using 1D and 2D NMR, as well as HRESIMS data. The results obtained by MTT assay showed different antitumor potential of these two jatrophanes. Compound 1 inhibited cell growth of non-small cell lung car­cinoma cell lines NCI-H460 and NCI-H460/R, as well as glioblastoma cell lines U87 and U87-TxR, while jatrophane 2 was almost completely inactive in the suppression of cancer cell growth in a given range of concentrations. The obtained results also showed that the isolated compounds have an inhibitory effect on P-glycoprotein, as well as that their inhibitory potential is similar.У претходном истраживању, петнаест дитерпена јатрофанског типа изоловано је из латекса Euphorbia nicaeensis. Њих четрнаест показала су се као снажни инхибитори P-гликопротеина (P-gp) у две MDR ћелијске линије рака (NCI-H460/R и DLD1-TxR). Циљ ове студије био је да се утврди да ли је и које јатрофанске дитерпене могуће изо- ловати из корена биљке, а затим испитивање њихове инхибиторне моћи на P-гликопро- теину одабраних ћелијских линија рака (NCI-H460, DLD1, U87, NCI-H460/R, DLD1-TxR и U87-TxR). Два претходно непозната јатрофана изолованa су из корена E. nicaeensis прикупљеног у Делиблатској пешчари. Структуре изолованих једињења одређене су применом 1D и 2D NMR метода, као и HRESIMS експеримента. Резултати добијени МТТ тестом показали су различит антиканцерогени потенцијал ова два јатрофана. Једињење 1 је инхибирало раст ћелија ћелијских линија неситноћелијског карцинома плућа NCI-H460 и NCI-H460/R, као и ћелијских линија глиобластома U87 и U87-TxR, док је јатрофан 2 био готово потпуно неефикасан у сузбијању растa ћелија карцинома у датом концентрационом опсегу. Добијени резултати су такође показали да 1 и 2 имају инхибиторно дејство на P-гликопротеин, као и да је њихов инхибиторни потенцијал сличан.Part of the theme issue honoring Professor Emeritus Slobodan Milosavljevićs 80th birthday

Analysis of benzoic and cinnamic acid derivatives of some medicinal plants in Serbia

Natural phenolics, which are ubiquitously distributed in plants, have been reported as functional factors in phytotherapy. We have examined phenolic compounds in the leaves and inflorescences of five significant medicinal plants of different plant families: Salvia officinalis (Lamiaceae); Achillea clypeolata (Asteraceae); Nymphaea alba (Nymphaeaceae); Rumex acetosella (Polygonaceae) and Allium ursinum (Alliaceae). The examined species were rich in total phenolics (up to 30.88 mg/g dry weight). According to their total phenolics contents, the plants can be arranged in the following order: A. clypeolata>N. alba>S. officinalis>R. acetosella>A. ursinum. Free phenolics prevailed in all species in comparison to the bound forms (63.72-82.68% of total phenolics). The highest content of total free phenolics was measured in the tissues of A. clypeolata and N. alba, and the lowest in A. ursinum. Five phenolic acids were isolated and measured. p-Coumaric and ferulic acids as derivatives of cinnamic acid prevailed in the leaves of R. acetosella and A. ursinum (up to 4.81%).Projekat ministarstva br. 17301

Uticaj folijarne ishrane na prinos i kvalitet soje proizvedene u organskoj proizvodnji

Organic agricultural production recorded a continuous growth in output and demand, globally speaking, in order to meet consumer needs for quality and safe food. The plots of the Institute for Field and Vegetable Crops in Bački Petrovac, the experiment performed soybean for method in organic production, in four replications in 2011. This paper studied the influence of foliar nutrition, with microbial fertilizer on the yield and chemical composition of grain, soybean varieties in NS Galina. We studied the following properties: soybean yield (kg/ha), content (%) and yield (kg/ha) of total protein and oil content of soybeans. Have been achieved very high yields of soybean in 5,746 amounted to an average kg/ha. Yields ranged from 5,604 kg/ha in the control variant to 5,887 kg/ha for foliar feeding options. Average grain yield was higher in the variants of foliar feeding of 283 kg/ha or 5.05% compared to the control variant. Differences defined in oil content soybean material between the control and foliar nutrition were not statistically significant, while the protein content was the same in both variants. Higher protein and oil yield in the was recorded as either a foliar nutrition. Were done, the values of the correlation coefficient between the studied traits. There was a significantly high positive correlation between yield and oil content (0.73**) and a negative correlation between yield and protein content (-0.35) and between oil and protein content in soybean (-0.45). The organic production of soybean foliar nutrition proved to be a successful method for improving the yield of soybean. Research to be continued.Organska poljoprivredna proizvodnja beleži kontinuirani rast obima proizvodnje i tražnje posmatrano u globalnim okvirima, u cilju zadovoljenja potreba potrošača za kvalitetnom i zdravstveno bezbednijom hranom. Na parcelama Instituta za ratarstvo i povrtarstvo, u Bačkom Petrovcu, izveden je ogled soje po metodama organske proizvodnje, u četiri ponavljanja, 2011. godine. U radu je proučavan uticaj folijarne ishrane sa mikrobiološkim đubrivom na prinos i hemijski sastav zrna, kod NS sorte Galina. Ispitivana su sledeća svojstva: prinos zrna soje (kg/ha), sadržaj (%) i prinos (kg/ha) ukupnih proteina i ulja u zrnu soje. Ostvareni su izuzetno visoki prinosi soje i u proseku su iznosili 5.746 kg/ha. Prinosi su varirali od 5.604 kg/ha u kontrolnoj varijanti do 5.887 kg/ha u varijanti folijarne ishrane. Prinos zrna soje u proseku bio je viši u varijanti folijarne ishrane za 283 kg/ha ili za 5,05 % u odnosu na kontrolnu varijantu. Definisane razlike sadržaja ulja u zrnu soje između kontrolnog materijala i folijarne ishrane nisu statistički značajne, dok je sadržaj proteina bio isti u obe varijante. Odrađene su vrednosti koeficijenta korelacije između ispitivanih svojstava. Zabeležena je pozitivna visoko statistički značajna korelacija između prinosa i sadržaja ulja (0,73**) i negativna korelacija između prinosa i sadržaja proteina (-0,35) kao i između sadržaja ulja i sadržaja proteina u zrnu soje (-0,45). U organskoj proizvodnji soje folijarna ishrana pokazala se kao moguć metod za poboljšanje prinosa soje. Ispitivanje treba nastaviti

Supplementary data for article: Šukalović, V.; Bogdan, A. E.; Tovilovic, G.; Ignjatovic, D.; Andrić, D.; Kostić-Rajačić, S.; Šoškić, V. N-{[2-(4-Phenyl-Piperazin-1-Yl)-Ethyl]-Phenyl}-Arylamides with Dopamine D-2 and 5-Hydroxytryptamine 5HT(1A) Activity: Synthesis, Testing, and Molecular Modeling. Archiv der Pharmazie 2013, 346 (10), 708–717. https://doi.org/10.1002/ardp.201300189

Supporting information for: [https://doi.org/10.1002/ardp.201300189]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1413