445 research outputs found

    On Recognizing Transparent Objects in Domestic Environments Using Fusion of Multiple Sensor Modalities

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    Current object recognition methods fail on object sets that include both diffuse, reflective and transparent materials, although they are very common in domestic scenarios. We show that a combination of cues from multiple sensor modalities, including specular reflectance and unavailable depth information, allows us to capture a larger subset of household objects by extending a state of the art object recognition method. This leads to a significant increase in robustness of recognition over a larger set of commonly used objects.Comment: 12 page

    Succession of Genetic Diversity of Botryococcus braunii (Trebouxiophyceae) in Two Japanese Reservoirs

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    AbstractBotryococcus braunii is a green colonial alga that produces large volumes of liquid hydrocarbon. Therefore, B. braunii is expected to be useful as an alternative fuel resource. Natural blooms of B. braunii have been recorded in several lakes and reservoirs. Elucidation of natural B. braunii blooming would provide important information for the development of an open-pond cultivation system. In this study, we periodically assessed the genetic diversity and colony density of B. braunii populations, along with several environmental parameters, in two Japanese reservoirs (provisionally called “N” and “S”) from December 2008 to December 2009. Reservoir N had low numbers of B. braunii colonies whereas Reservoir S was characterized by periodic density increases that occurred in December 2008, and in March, September, and December 2009. Population genetics analysis using specific environmental sequences (PGA-SES method) was conducted for B. braunii populations for the first time. Among the B. braunii-dominated samples of Reservoir S, high levels of genetic diversity were observed in December 2008 and March 2009, whereas the diversity levels in September and December 2009 were low. The results suggest that B. braunii periodicity can be categorized into a high genetic diversity type and a low genetic diversity type. The high genetic diversity type may be caused by simultaneous growth of many genotypes, whereas the low genetic diversity type seems to be explained by increases in the cell density of only a few adapted genotypes

    An Analysis of Uniform Shear Flow Past a Porous Plate Attached to a Plane Surface

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    This is a good first study on the applicability of the wake-source model to shear flow around and through a screen. Since previous work [4] dealt with uniform flow, the authors have properly chosen shear flow as the next step in extending the work. Moreover, shear flow is probably more important than uniform flow in practical situations, and so this study should be of interest to designers who need quantitative information on the effectiveness of wind breaks and the like. To be truly useful, however, this study should be continued in order to vary plate porosity and wind shear rate. The present experiments were carried out for a single plate and for one upstream velocity profile. The pressure-drop coefficient K for the plate was 18.1, which is higher than values for normal porous materials like woven screens, and the average shear rate G of the velocity profile was 216 s" 1 , which corresponds to a moderate 53 percent change in velocity from the bottom to the top of the screen. These conditions produced a downstream velocity profile that was satisfactorily predicted by the wake-source model, but this tells us little about the accuracy of the model for other conditions. Additional experimental results would not only establish the range of validity of the model but would also provide useful data for design purposes. For the latter, the quantity of interest is likely the reduction of velocity produced by the windbreak, and thus it would be beneficial to have a plot, say, of mean velocity reduction as a function of the screen coefficient K, with G as a free parameter. I hope the present work is the initial step in an endeavour of this sort. The authors do, in fact, infer that the study is being continued and to that end I would suggest several changes in future work. First, the mapping of the velocity field upstream of the plate is presently incomplete and the matching location too arbitrary. It would be interesting to learn, for example, the effect on predicted downstream profile if the matching were carried out at x/h = -1.1, say, and not at x/h = -2.2. Secondly, it should be straightforward to measure the plate's pressure-drop coefficient and so verify the value calculated in the text. Finally, it would be useful to investigate non-uniform shear flows. Uniform shear is the logical case after uniform flow, as mentioned above, but it is not true that the theory demands a uniform shear flow which has a constant vorticity. The model requires only that the flow be inviscid, not irrotational. Consequently the approaching flow may hav

    Responses of picoplankton to nutrient perturbation in the South China Sea, with special reference to the coast-wards distribution of Prochlorococcus

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    Responses of Prochlorococcus (Pro), Synechococcus (Syn), pico-eukaryotes (Euk) and heterotrophic bacteria (Bact) in pelagic marine ecosystems to external nutrient perturbations were examined using nitrogen- (N), phosphorus- (P), iron- (Fe), and cobalt- (Co) enriched incubations in the South China Sea in November 1997. Variations in abundance of the 4 groups of microorganism and cellular pigment content of the autotrophs during incubation were followed by flow-cytometric measurements for seven days. During the incubation, Syn and Euk showed a relatively higher demand on Fe and N, while Pro required higher levels of Co and P. The Fe was inadequate for all the organisms in the deep euphotic zone (75 m) of the study area. The experimental results also implied that biological interaction among the organisms played a role in the community structure shift during the incubation. It seemed that besides the effects of temperature, there are some other physical and chemical limitations as well as impacts from biological interactions on Pro distribution in coast waters

    Citizen Science Observation of a Gamma‐Ray Glow Associated With the Initiation of a Lightning Flash

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    シチズンサイエンスで挑む雷の謎 --宇宙線と雷雲の相互作用は、雷の始まりに影響を与えるのか?--. 京都大学プレスリリース. 2023-07-10.Zeus also plays billiards: Citizen-supported Thundercloud Project may lead to better understanding of lightning's origins. 京都大学プレスリリース. 2023-07-12.Gamma-ray glows are observational evidence of relativistic electron acceleration due to the electric field in thunderclouds. However, it is yet to be understood whether such relativistic electrons contribute to the initiation of lightning discharges. To tackle this question, we started the citizen science “Thundercloud Project, ” where we map radiation measurements of glows from winter thunderclouds along Japan's sea coast area. We developed and deployed 58 compact gamma-ray monitors at the end of 2021. On 30 December 2021, five monitors simultaneously detected a glow with its radiation distribution horizontally extending for 2 km. The glow terminated coinciding with a lightning flash at 04:08:34 JST, which was recorded by the two radio-band lightning mapping systems, FALMA and DALMA. The initial discharges during the preliminary breakdown started above the glow, that is, in vicinity of the electron acceleration site. This result provides one example of possible connections between electron acceleration and lightning initiation

    Initiation of human colon cancer cell proliferation by trypsin acting at protease-activated receptor-2

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    The protease-activated receptor-2 (PAR-2) is a G protein-coupled receptor that is cleaved and activated by trypsin. We investigated the expression of PAR-2 and the role of trypsin in cell proliferation in human colon cancer cell lines. A total of 10 cell lines were tested for expression of PAR-2 mRNA by Northern blot and RT-PCR. PAR-2 protein was detected by immunofluorescence. Trypsin and the peptide agonist SLIGKV (AP2) were tested for their ability to induce calcium mobilization and to promote cell proliferation on serum-deprived cells. PAR-2 mRNA was detected by Northern blot analysis in 6 out of 10 cell lines [HT-29, Cl.19A, Caco-2, SW480, HCT-8 and T84]. Other cell lines expressed low levels of transcripts, which were detected only by RT-PCR. Further results were obtained with HT-29 cells: (1) PAR-2 protein is expressed at the cell surface; (2) an increase in intracellular calcium concentration was observed upon trypsin (1–100 nM) or AP2 (10–100 μM) challenges; (3) cells grown in serum-deprived media supplemented with trypsin (0.1–1 nM) or AP2 (1–300 μM) exhibited important mitogenic responses (3-fold increase of cell number). Proliferative effects of trypsin or AP2 were also observed in other cell lines expressing PAR-2. These data show that subnanomolar concentrations of trypsin, acting at PAR-2, promoted the proliferation of human colon cancer cells. The results of this study indicate that trypsin could be considered as a growth factor and unravel a new mechanism whereby serine proteases control colon tumours. © 2001 Cancer Research Campaign http://www.bjcancer.co

    The Heterotrimeric Laminin Coiled-Coil Domain Exerts Anti-Adhesive Effects and Induces a Pro-Invasive Phenotype

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    Laminins are large heterotrimeric cross-shaped extracellular matrix glycoproteins with terminal globular domains and a coiled-coil region through which the three chains are assembled and covalently linked. Laminins are key components of basement membranes, and they serve as attachment sites for cell adhesion, migration and proliferation. In this work, we produced a recombinant fragment comprising the entire laminin coiled-coil of the α1-, β1-, and γ1-chains that assemble into a stable heterotrimeric coiled-coil structure independently of the rest of the molecule. This domain was biologically active and not only failed to serve as a substrate for cell attachment, spreading and focal adhesion formation but also inhibited cell adhesion to laminin when added to cells in a soluble form at the time of seeding. Furthermore, gene array expression profiling in cells cultured in the presence of the laminin coiled-coil domain revealed up-regulation of genes involved in cell motility and invasion. These findings were confirmed by real-time quantitative PCR and zymography assays. In conclusion, this study shows for the first time that the laminin coiled-coil domain displays anti-adhesive functions and has potential implications for cell migration during matrix remodeling
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