22 research outputs found
ΠΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ ΠΏΡΠΎΠ³Π½ΠΎΠ·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠΏΠ° ΡΠ²Π΅Π°Π»ΡΠ½ΡΡ ΠΌΠ΅Π»Π°Π½ΠΎΠΌ Π±Π΅Π· ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ ΠΈΠ½Π²Π°Π·ΠΈΠ²Π½ΡΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ
Π Π΅Π·ΡΠΌΠ΅. Π‘ ΠΏΠΎΠΌΠΎΡΡΡ Π΄ΠΈΡΠΊΡΠΈΠΌΠΈΠ½Π°Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π° Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠΏΠ° ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ ΡΠ²Π΅Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΠΊΡΠ° Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ ΠΊΠΎΠΌΠ±ΠΈΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ (ΡΠΎΡΠΎΠΊΠΎΠ°Π³ΡΠ»ΡΡΠΈΡ + Π±ΡΠ°Ρ
ΠΈΡΠ΅ΡΠ°ΠΏΠΈΡ) Π»Π΅ΡΠ΅Π½ΠΈΡ. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π° Π²ΡΡΠΎΠΊΠΎΠ·Π½Π°ΡΠΈΠΌΠ°Ρ (l = 0,08; Ρ = 0,002) Π΄ΠΈΡΠΊΡΠΈΠΌΠΈΠ½Π°Π½ΡΠ½Π°Ρ ΠΌΠΎΠ΄Π΅Π»Ρ, Π²ΠΊΠ»ΡΡΠ°ΡΡΠ°Ρ ΡΡΠ΄ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ
(ΡΡΠ΅ΠΏΠ΅Π½Ρ ΠΏΠΈΠ³ΠΌΠ΅Π½ΡΠ°ΡΠΈΠΈ, ΠΏΠΎΠ», ΡΠΊΠΎΡΠΎΡΡΡ ΡΠΎΡΡΠ° ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ) ΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
(ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ Π’- ΠΈ Π-Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ², ΠΏΡΠΎΡΠ΅Π½Ρ Π’-Ρ
Π΅Π»ΠΏΠ΅ΡΠΎΠ² ΠΈ Π΄Ρ.) ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ. ΠΡΠΎΠ±ΠΎΠ΅ ΠΌΠ΅ΡΡΠΎ Π² ΠΌΠΎΠ΄Π΅Π»ΠΈ Π·Π°Π½ΠΈΠΌΠ°ΡΡ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΈ, Π² Π½Π°ΠΈΠ±ΠΎΠ»ΡΡΠ΅ΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ ΠΎΡΡΠ°ΠΆΠ°ΡΡΠΈΠ΅ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ ΡΠ²Π΅Π°Π»ΡΠ½ΡΡ
ΠΌΠ΅Π»Π°Π½ΠΎΠΌ ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠ³ΠΎ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π°, Π° ΠΈΠΌΠ΅Π½Π½ΠΎ β ΡΠΊΠΎΡΠΎΡΡΡ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΠ°Π·ΠΌΠ΅ΡΠ° ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΈ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½ΠΈΡΠ΅ΡΠ°.
ΠΠ»ΡΡΠ΅Π²ΡΠ΅ ΡΠ»ΠΎΠ²Π°: ΡΠ²Π΅Π°Π»ΡΠ½Π°Ρ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΠ°, ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΠΉ ΡΠΈΠΏ, ΠΊΠ»ΠΈΠ½ΠΈΠΊΠΎ-ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅, ΠΈΠΌΠΌΡΠ½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ, Π΄ΠΈΡΠΊΡΠΈΠΌΠΈΠ½Π°Π½ΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ·.Summary. Application of the discriminant analysis shows that it is possible to define the cell type of melanoma of uveal tract of the eye in the process of combined (photocoagulation + brachytherapy) treatment. A highly reliable (l= 0,08; Ρ = 0,002) discriminant model was elaborated, involving a number of both clinical (pigmentation level, gender, melanoma growth rate) and immunological (number of T and B lymphocytes, T helper rate, etc.) indicators. In this model, especially important are those traits that most pronouncedly reflect the biological peculiarities of uveal melanomas of various cellular compositions, namely β the pace of tumor size growth in the process of treatment and changes in cell immunity indicators.
Key Words: uveal melanoma, cell type, clinical and morphological, immunological indicators, discriminant analysis
Dissecting the Wnt secretion pathway: key questions on the modification and intracellular trafficking of Wnt proteins
The Wnt family of signalling proteins has essential functions in development and adult tissue homoeostasis throughout the animal kingdom. Although signalling cascades triggered by Wnt proteins have been extensively studied, much remains to be learned about how Wnts are produced and secreted. Over the past few years, it has become clear that the secretion of Wnt proteins requires a specialized trafficking pathway. As this pathway has been discussed in two recent reviews (Lorenowicz & Korswagen 2009, Port & Basler 2010), we will focus our discussion on the key questions that need to be addressed to gain a more complete understanding of the mechanism and regulation of this essential secretion pathway
Sorting nexins provide diversity for retromer-dependent trafficking events
Sorting nexins are a large family of evolutionarily conserved phosphoinositide-binding proteins that have fundamental roles in orchestrating cargo sorting through the membranous maze that is the endosomal network. One ancient group of complexes that contain sorting nexins is the retromer. Here we discuss how retromer complexes regulate endosomal sorting, and describe how this is generating exciting new insight into the central role played by endosomal sorting in development and homeostasis of normal tissues
Sailing with the Wnt: charting the Wnt processing and secretion route
Wnt proteins are members of a highly conserved family of signalling molecules that play a central role in development and disease. During the past years, the different signalling pathways that are triggered by Wnt proteins have been studied in detail, but it is still largely unknown how a functional Wnt protein is produced and secreted. The recent finding that Wnt proteins are post-translationally modified and the discovery of the Wnt binding protein Wntless and its trafficking by the retromer complex show that Wnt secretion is a complex and highly regulated process. In this review, we will give an overview of the Wnt maturation and secretion pathway and discuss how this process may influence the spreading and signalling activity of Wnt
Sailing with the Wnt: charting the Wnt processing and secretion route
Wnt proteins are members of a highly conserved family of signalling molecules that play a central role in development and disease. During the past years, the different signalling pathways that are triggered by Wnt proteins have been studied in detail, but it is still largely unknown how a functional Wnt protein is produced and secreted. The recent finding that Wnt proteins are post-translationally modified and the discovery of the Wnt binding protein Wntless and its trafficking by the retromer complex show that Wnt secretion is a complex and highly regulated process. In this review, we will give an overview of the Wnt maturation and secretion pathway and discuss how this process may influence the spreading and signalling activity of Wnt.
Wnt signaling requires retromer-dependent recycling of MIG-14/Wntless in Wnt-producing cells.
Wnt proteins are secreted signaling molecules that play a central role in development and adult tissue homeostasis. We have previously shown that Wnt signaling requires retromer function in Wnt-producing cells. The retromer is a multiprotein complex that mediates endosome-to-Golgi transport of specific sorting receptors. MIG-14/Wls is a conserved transmembrane protein that binds Wnt and is required in Wnt-producing cells for Wnt secretion. Here, we demonstrate that in the absence of retromer function, MIG-14/Wls is degraded in lysosomes and becomes limiting for Wnt signaling. We show that retromer-dependent recycling of MIG-14/Wls is part of a trafficking pathway that retrieves MIG-14/Wls from the plasma membrane. We propose that MIG-14/Wls cycles between the Golgi and the plasma membrane to mediate Wnt secretion. Regulation of this transport pathway may enable Wnt-producing cells to control the range of Wnt signaling in the tissue