15 research outputs found
Decellularisation and Histological Characterisation of Porcine Peripheral Nerves
Peripheral nerve injuries affect a large proportion of the global population, often causing significant morbidity and loss of function. Current treatment strategies include the use of implantable nerve guide conduits (NGC’s) to direct regenerating axons between the proximal and distal ends of the nerve gap. However, NGC’s are limited in their effectiveness at promoting regeneration Current NGCs are not suitable as substrates for supporting either neuronal or Schwann cell growth, as they lack an architecture similar to that of the native extracellular matrix (ECM) of the nerve. The aim of this study was to create an acellular porcine peripheral nerve using a novel decellularisation protocol, in order to eliminate the immunogenic cellular components of the tissue, while preserving the three-dimensional histoarchitecture and ECM components. Porcine peripheral nerve (sciatic branches were decellularised using a low concentration (0.1 %; w/v) sodium dodecyl sulphate in conjunction with hypotonic buffers and protease inhibitors, and then sterilised using 0.1 % (v/v) peracetic acid. Quantitative and qualitative analysis revealed a ≥95 % (w/w) reduction in DNA content as well as preservation of the nerve fascicles and connective tissue. Acellular nerves were shown to have retained key ECM components such as collagen, laminin and fibronectin. Slow strain rate to failure testing demonstrated the biomechanical properties of acellular nerves to be comparable to fresh controls. In conclusion, we report the production of a biocompatible, biomechanically functional acellular scaffold, which may have use in peripheral nerve repair
Heart valve tissue‐derived hydrogels: Preparation and characterization of mitral valve chordae, aortic valve, and mitral valve gels
Development and characterisation of a low-concentration sodium dodecyl sulphate decellularised porcine dermis
Comparison of small-diameter decellularized scaffolds from the aorta and carotid artery of pigs
Decellularization of human donor aortic and pulmonary valved conduits using low concentration sodium dodecyl sulfate
Development and Characterization of a Porcine Mitral Valve Scaffold for Tissue Engineering
This paper is in closed access.Decellularized scaffolds represent a promising alternative for mitral valve (MV) replacement. This work developed and characterized a protocol for the decellularization of
whole MVs. Porcine MVs were decellularized with 0.5% (w/
v) SDS and 0.5% (w/v) SD and sterilized with 0.1% (v/v) PAA.
Decellularized samples were seeded with human foreskin fibroblasts and human adipose-derived stem cells to investigate
cellular repopulation and infiltration, and with human colonyforming endothelial cells to investigate collagen IV formation.
Histology revealed an acellular scaffold with a generally conserved histoarchitecture, but collagen IV loss. Following
decellularization, no significant changes were observed in
the hydroxyproline content, but there was a significant reduction in the glycosaminoglycan content. SEM/TEM analysis
confirmed cellular removal and loss of some extracellular matrix components. Collagen and elastin were generally
preserved. The endothelial cells produced newly formed collagen IVon the non-cytotoxic scaffold. The protocol produced
acellular scaffolds with generally preserved histoarchitecture,
biochemistry, and biomechanics