53 research outputs found
Utjecaj glutaraldehida na svojstva želatinskih filmova
In this work gelatin film was prepared from cow\u27s bone. In order to increase mechanical stability of the prepared transparent film as well as decrease its swelling, glutaraldehyde (GTA) was used. Different mass fractions of GTA were utilized. It was observed that at w = 0.18 % of GTA the load at break of the film is δ= 53.7 N and the solubility decreased. The solubility of the film was measured as a dependant parameter of the swelling behavior. In this case it was decreased from 389 % to 156 % at 5 min for gelatin films without GTA and with 0.18 % GTA, respectively. FTIR spectroscopy results showed a peak for crosslinked gelatin at ΰ = 1650 cm-1. It means the crosslinking between gelatin and GTA has taken place. SEM micrographs confirm the porosity has decreased by increasing the GTA fraction, which is an indication of higher strength.U ovom je radu pripremljen želatinski film od kravlje kosti. Da bi se povećala mehanička stabilnost pripremljenog transparentnog filma kao i smanjilo njegovo bubrenje, upotrijebljen je glutaraldehid (GTA). Upotrijebljeni su različiti maseni udjeli mase GTA. Uočeno je da kod 0,18 % GTA prekidna sila filma iznosi 53,7 N i da se povećala topljivost. Topljivost filma izmjerena je kao parametar ovisan o ponašanju pri bubrenju. U ovom slučaju smanjio se s 389 % na 156 % kod 5 minuta za želatinske filmove bez GTA odn. s 0,18 % GTA. Rezultati spektroskopije FTIR pokazali su maksimum za umreženu želatinu kod 1650 cm-1. To znači da je došlo do umrežavanja između želatine i GTA. Mikroskopske slike SEM potvrđuju da se poroznost smanjila povećanjem dijela GTA, što je pokazatelj veće čvrstoće
Updated standardized definitions for efficacy endpoints in adjuvant breast cancer clinical trials: STEEP Version 2.0
Purpose The Standardized Definitions for Efficacy End Points (STEEP) criteria, established in 2007, provide standardized definitions of adjuvant breast cancer clinical trial end points. Given the evolution of breast cancer clinical trials and improvements in outcomes, a panel of experts reviewed the STEEP criteria to determine whether modifications are needed.Methods We conducted systematic searches of ClinicalTrials.gov for adjuvant systemic and local-regional therapy trials for breast cancer to investigate if the primary end points reported met STEEP criteria. On the basis of common STEEP deviations, we performed a series of simulations to evaluate the effect of excluding non-breast cancer deaths and new nonbreast primary cancers from the invasive disease-free survival end point.Results Among 11 phase III breast cancer trials with primary efficacy end points, three had primary end points that followed STEEP criteria, four used STEEP definitions but not the corresponding end point names, and four used end points that were not included in the original STEEP manuscript. Simulation modeling demonstrated that inclusion of second nonbreast primary cancer can increase the probability of incorrect inferences, can decrease power to detect clinically relevant efficacy effects, and may mask differences in recurrence rates, especially when recurrence rates are low.Conclusion We recommend an additional end point, invasive breast cancer-free survival, which includes all invasive disease-free survival events except second nonbreast primary cancers. This end point should be considered for trials in which the toxicities of agents are well-known and where the risk of second primary cancer is small. Additionally, we provide end point recommendations for local therapy trials, low-risk populations, noninferiority trials, and trials incorporating patient-reported outcomes
Discovery of the inhibitory effect of a phosphatidylinositol derivative on P-glycoprotein by virtual screening followed by <i>in vitro</i> cellular studies
P-glycoprotein is capable of effluxing a broad range of cytosolic and membrane penetrating xenobiotic substrates, thus leading to multi-drug resistance and posing a threat for the therapeutic treatment of several diseases, including cancer and central nervous disorders. Herein, a virtual screening campaign followed by experimental validation in Caco-2, MDKCII, and MDKCII mdr1 transfected cell lines has been conducted for the identification of novel phospholipids with P-gp transportation inhibitory activity. Phosphatidylinositol-(1,2-dioctanoyl)-sodium salt (8∶0 PI) was found to significantly inhibit transmembrane P-gp transportation in vitro in a reproducible-, cell line-, and substrate-independent manner. Further tests are needed to determine whether this and other phosphatidylinositols could be co-administered with oral drugs to successfully increase their bioavailability. Moreover, as phosphatidylinositols and phosphoinositides are present in the human diet and are known to play an important role in signal transduction and cell motility, our finding could be of substantial interest for nutrition science as well
ABC-transporter upregulation mediates resistance to the CDK7 inhibitors THZ1 and ICEC0942.
The CDK7 inhibitors (CDK7i) ICEC0942 and THZ1, are promising new cancer therapeutics. Resistance to targeted drugs frequently compromises cancer treatment. We sought to identify mechanisms by which cancer cells may become resistant to CDK7i. Resistant lines were established through continuous drug selection. ABC-transporter copy number, expression and activity were examined using real-time PCR, immunoblotting and flow cytometry. Drug responses were measured using growth assays. ABCB1 was upregulated in ICEC0942-resistant cells and there was cross-resistance to THZ1. THZ1-resistant cells upregulated ABCG2 but remained sensitive to ICEC0942. Drug resistance in both cell lines was reversible upon inhibition of ABC-transporters. CDK7i response was altered in adriamycin- and mitoxantrone-resistant cell lines demonstrating ABC-transporter upregulation. ABCB1 expression correlated with ICEC0942 and THZ1 response, and ABCG2 expression with THZ2 response, in a panel of cancer cell lines. We have identified ABCB1 upregulation as a common mechanism of resistance to ICEC0942 and THZ1, and confirmed that ABCG2 upregulation is a mechanism of resistance to THZ1. The identification of potential mechanisms of CDK7i resistance and differences in susceptibility of ICEC0942 and THZ1 to ABC-transporters, may help guide their future clinical use
Dendritic cell tumor in a salivary gland lymph node: a rare differential diagnosis of salivary gland neoplasms
Failure Diagnosis of Wind Turbine Bearing Using Feature Extraction and a Neuro-Fuzzy Inference System (ANFIS)
Bearing failures are the most common type of malfunction in wind turbines. As such, isolating these defects enables maintenance scheduling in advance; hence, preventing further damage to turbines. This paper introduces a new fault detection and diagnosis (FDD) method to isolate two types of bearing failures in Wind turbines (WTs). The proposed FDD method consists of a feature extraction/feature selection and an adaptive neuro-fuzzy inference system (ANFIS) method. The feature extraction and selection phase identifies proper features to capture the nonlinear dynamics of the failure. Then, the ANFIS classifier diagnoses the failure type using the extracted features. Several experimental test studies with the historical data of wind farms in South-western Ontario are performed to evaluate the performance of the FDD system. Test results indicate that the proposed monitoring system is accurate and effective
Firms in Green Public Procurement: Financial Strength Indicators’ Impact on Contract Awards and Its Repercussion on Financial Strength
We examine whether the financial strength of companies, in particular, small and medium-sized enterprises (SMEs) is causally linked to the award of a public procurement contract (PP), especially in the environmentally friendly green area (GPP). For this purpose, we build a combined procurement company data set from the Tenders Electronic Daily (TED) and the SME database AMADEUS, which includes ten European countries. First, we apply probit models to investigate whether the probability of winning the public tender depends on the company’s financial strength. We then use the flexpanel DiD approach to investigate the question of whether the award has an impact on the future financial strength of the successful company. On the one hand, we find that a lower equity ratio and a higher short-term debt ratio increase the probability of being successful in a public tender. On the other hand, a success means that the companies can continue to work after the award with a lower equity ratio than comparable companies without an award, regardless of whether the company was successful in a traditional or a green public tender. We conclude from this that the success in a PP is a substitute for a firm’s own financial strength and thus facilitates access to external financing
The Effect of Glutaraldehyde on the Properties of Gelatin Films
In this work gelatin film was prepared from cow's bone. In order to increase mechanical stability of the prepared transparent film as well as decrease its swelling, glutaraldehyde (GTA) was used. Different mass fractions of GTA were utilized. It was observed that at w = 0.18 % of GTA the load at break of the film is = 53.7 N and the solubility decreased. The solubility of the film was measured as a dependant parameter of the swelling behavior. In this case it was decreased from 389 % to 156 % at 5 min for gelatin films without GTA and with 0.18 % GTA, respectively. FTIR spectroscopy results showed a peak for crosslinked gelatin at ΰ = 1650 cm<sup>-1</sup>. It means the crosslinking between gelatin and GTA has taken place. SEM micrographs confirm the porosity has decreased by increasing the GTA fraction, which is an indication of higher strength
Photo-Fenton oxidative of pharmaceutical wastewater containing meropenem and ceftriaxone antibiotics: influential factors, feasibility, and biodegradability studies
Effect of Pin Geometry on the Mechanical Strength of Friction-Stir-Welded Polypropylene Composite Plates
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