PRES AS A COMPLICATION OF A MODERATE PREECLAMPSIA: CASE REPORT AND SHORT REVIEW OF LITERATURE

We present a case of puerpera with two major complications of preeclampsia: PRES manifested by eclampsia and HELLP syndrome. PRES is neuroradiological entity characterized by hypertension, altered mental status, visual disturbances, headache and generalized seizures together with characteristic findings on cerebral magnetic resonance imaging scan. An important fact considering PRES (which also happened to our patient) is that it can be developed without significant rise in blood pressure, in a situation of severe endothelial injury with diminished cerebral autoregulatory capacity. Another consequence of vascular endotheliosis that developed in our patient was HELLP syndrome. Although the complications are severe, this state is usually completely reversible presuming that prompt diagnosis and adequate therapy were timely undertaken, as in the case we report here.Key words: Eclampsia, posterior reversible encephalopathy syndrome, HELLP syndrom

Antioksidativna aktivnost metanolskog ekstrakta lista biljke Saxifraga Rotundifolia L.

The aim of this study was to investigate the potential antioxidant activity of the methanol leaf extract of Saxifraga rotundifolia L., depending on the total quantity of phenols, flavonoids and flavonol. The tested extracts showed high phenolic content (324.39 mg GA/g) and small amounts of flavonoids (36.81 mg RU/g) and flavonols (11.52 mg RU/g). The antioxidant activity was determined by a series of in vitro methods. The tested extracts showed high level of total antioxidant activity (509.78 mg AA/g), and high degree of ABTS+ and DPPH "scavenger" activity with IC50 value of 48.76 μg/mL for ABTS+ method and 30.39 μg/mL for DPPH method. The results indicate that the tested extract has significant antioxidant activity and can be a source of natural antioxidants.Cilj rada je bio da se ispita potencijalna antioksidativna aktivnost metanolskog ekstrakta lista biljke Saxifraga rotundifolia L. u zavisnosti od ukupne količine fenola, flavonoida i flavonola. Ispitivani ekstrakt pokazao je visok sadržaj fenola (324,39 mg GA/g) i manje količine flavonoida (36,81 mg RU/g) i flavonola (11,52 mg RU/g). Antioksidativna aktivnost određena je serijom in vitro metoda. Ispitivani ekstrakt pokazuje visok nivo ukupne antioksidativne aktivnosti (509,78 mg AA/g), kao i visok stepen АBTS+. i DPPH ,,skevindžer“ aktivnosti sa IC50 vrednošću od 48,76 μg/mL za ABTS+. i 30,39 μg/mL za DPPH metodu. Dobijeni rezultati ukazuju da ispitivani ekstrakt poseduje značajnu antioksidativnu aktivnost i može biti izvor prirodnih antioksidanata

Stabilnost ekstrakta korena suručice i efekat na sprečavanje lipidne oksidacije u mesu

Nowadays, consumers are demanding more natural foods, obliging the industry to include natural antioxidants in foods and synthetic antioxidants have been rejected because of their carcinogenicity. Natural antioxidants have been used instead of synthetic to retard lipid oxidation in foods to improve their quality and nutritional value. The aim of this work was to evaluate pH and termal stability, and effect on lipid oxidation in meat-model system of methanolic extract of Filipendula hexapetala Gilib. Results suggest that the stability of the extract is expressed in a variety of pH and thermal conditions. The root extract of F. hexapetala exerted a concentration-dependent antioxidant activity in a meat model system, lower than synthetic antioxidant BHT.Danas je u ishrani zastupljen veliki broj prirodnih proizvoda, tako da prehrambena industrija sve više koristi prirodne antioksidante, a sintetički antioksidanti se izbacuju iz upotrebe zbog njihove kancerogenosti. Prirodni antioksidanti se koriste umesto sintetičkih da uspore oksidaciju lipida, poboljšaju kvalitet i nutritivnu vrednost namirnica. Cilj ovog rada bio je određivanje pH i termalne stabilnosti, kao i efekta na lipidnu oksidaciju u mesu metanolskog ekstrakta korena biljke Filipendula hexapetala Gilib. Rezultati ukazuju da je stabilnost ekstrakta izražena u različitim pH i termalnim uslovima. Ekstrakt korena F. hexapetala pokazao je koncentraciono-zavisnu antioksidativnu aktivnost u meso model-sistemu, nešto nižu u odnosu na sintetički antioksidant BHT

Correlation between ECG changes and early left ventricular remodeling in preadolescent footballers

The aim of this study was to assess the early electrocardiogram (ECG) changes induced by physical training in preadolescent elite footballers. This study included 94 preadolescent highly trained male footballers (FG) competing in Serbian Football League (minimum of 7 training hours/week) and 47 age-matched healthy male controls (less than 2 training hours/week) (CG). They were screened by ECG and echocardiography at a tertiary referral cardio center. Sokolow–Lyon index was used as a voltage electrocardiographic criterion for left ventricular hypertrophy diagnosis. Characteristic ECG intervals and voltage were compared and reference range was given for preadolescent footballers. Highly significant differences between FG and CG were registered in all ECG parameters: P-wave voltage (p 1–2 + R V5–6 (p  0.05). During 6-year follow-up period, there was no adverse cardiac event in these footballers. None of them expressed pathological ECG changes. Benign ECG changes are presented in the early stage of athlete’s heart remodeling, but they are not related to pathological ECG changes and they should be regarded as ECG pattern of LV remodeling

Comparison of Antioxidant Effects of Honey, Glibenclamide, Metformin, and Their Combinations in the Kidneys of Streptozotocin-Induced Diabetic Rats

Hyperglycemia-induced increase in oxidative stress is implicated in diabetic complications. This study investigated the effect of metformin and/or glibenclamide in combination with honey on antioxidant enzymes and oxidative stress markers in the kidneys of streptozotocin (60 mg/kg; intraperitoneal)-induced diabetic rats. Diabetic rats were randomized into eight groups of five to seven rats and received distilled water (0.5 mL); honey (1.0 g/kg); metformin (100 mg/kg); metformin (100 mg/kg) and honey (1.0 g/kg); glibenclamide (0.6 mg/kg); glibenclamide (0.6 mg/kg) and honey (1.0 g/kg); metformin (100 mg/kg) and glibenclamide (0.6 mg/kg); or metformin (100 mg/kg), glibenclamide (0.6 mg/kg) and honey (1.0 g/kg) orally once daily for four weeks. Malondialdehyde (MDA) levels, glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were significantly elevated while catalase (CAT) activity, total antioxidant status (TAS), reduced glutathione (GSH), and GSH:oxidized glutathione (GSSG) ratio was significantly reduced in the diabetic kidneys. CAT, glutathione reductase (GR), TAS, and GSH remained significantly reduced in the diabetic rats treated with metformin and/or glibenclamide. In contrast, metformin or glibenclamide combined with honey significantly increased CAT, GR, TAS, and GSH. These results suggest that combination of honey with metformin or glibenclamide might offer additional antioxidant effect to these drugs. This might reduce oxidative stress-mediated damage in diabetic kidneys

Increased oxidative stress associated with the severity of the liver disease in various forms of hepatitis B virus infection

BACKGROUND: Oxidative stress can be defined as an increase in oxidants and/or a decrease in antioxidant capacity. There is limited information about the oxidative status in subjects with hepatitis B virus infection. We aimed to evaluate the oxidative status in patients with various clinical forms of chronic hepatitis B infection. METHODS: Seventy-six patients with hepatitis B virus infection, in whom 33 with chronic hepatitis, 31 inactive carriers and 12 with cirrhosis, and 16 healthy subjects were enrolled. Total antioxidant response and total peroxide level measurement, and calculation of oxidative stress index were performed in all participants. RESULTS: Total antioxidant response was significantly lower in cirrhotics than inactive HbsAg carriers and controls (p = 0.008 and p = 0.008, respectively). Total peroxide level and oxidative stress index was significantly higher in cirrhotic (p < 0.001, both) and chronic hepatitis B subjects (p < 0.001, both) than inactive HbsAg carriers and controls. Total antioxidant response was comparable in chronic hepatitis B subjects, inactive HbsAg carriers and controls (both, p > 0.05/6). Total peroxide level and oxidative stress index were also comparable in inactive HBsAg carriers and controls (both, p > 0.05/6). Serum alanine amino transferase level was positively correlated with total peroxide level and oxidative stress index only in chronic hepatitis B subjects (p = 0.002, r = 0.519 and p = 0.008, r = 0.453, respectively). CONCLUSION: Oxidative stress occurs secondarily to increased total lipid peroxidation and inadequate total antioxidant response and is related to severity of the disease and replication status of virus in hepatitis B infection

Nanoparticles of Selenium as Species with Stronger Physiological Effects in Sheep in Comparison with Sodium Selenite

The present study was designed to compare the effects of nano red selenium and sodium selenite on the antioxidative activities of neutrophils and the hematological parameters in sheep. Fifteen sheep were randomly allocated into three groups. Groups 1 and 2 received selenium nanoparticles orally at 1 mg/kg and sodium selenite at 1 mg Se/kg for 10 consecutive days; group 3 served as the control. To assess the degrees of oxidative stress and of lipid peroxidation of the cellular membranes, the levels of thiobarbituric acid reactive substances (TBARS) were determined in serum samples that were collected at different supplementation intervals, i.e., after 0, 10, 20, and 30 days. In addition, hematological parameters in the serum samples were measured by routine procedures. It was found that TBARS levels in groups 1 and 2 were significantly higher on days 20 and 30 compared to the basal level on day 0. It was also found that on day 30, the TBARS activities in both treated groups were significantly higher than those of the controls (P < 0.05). These findings may explain the seemingly paradoxical effects of supplemental selenium on the indicators of oxidative stress, as the levels of TBARS were generally expected to decrease in the presence of selenium. There were no significant differences between the PCV and RBC values in the three groups. The white blood cell count (WBC) in group 1 showed a significant increase on days 20 and 30 in comparison with the control group. However, in group 2, there was a significant increase of the WBC value just on day 20 in comparison with the control group. Also, there were significant increases of the neutrophil counts and significant decreases of the lymphocyte counts on day 10 in group 1, in comparison with those in group 2 and controls, and on days 20 and 30 in groups 1 and 2 in comparison with those in the control group