Seasonal effects on HPLC-DAD-UV and UPLC-ESI-MS fingerprints and analgesic activities of vernonia condensata baker extracts

Vernonia condensata Baker leaves have different uses in Brazilian folk medicine, including as analgesic and anti-inflammatory agents. The purpose of this study was to evaluate the seasonal effects on their high performance liquid chromatography with a diode array detector (HPLC-DAD-UV) and ultra-performance liquid chromatography coupled to a mass spectrometer with an electrospray interface (UPLC-ESI-MS) fingerprints, as well as their analgesic activities in mice. There were significant seasonal effects on the relative abundances of the metabolites of the V. condensate leaves as well as on their activities. Analgesic activities in the writhing test were observed with the polar fraction of the leaf extracts collected in autumn, winter and summer (400 mg kg(-1)); and with the intermediate fraction of leaves collected in autumn (25 and 400 mg kg(-1)) and in the summer (100 mg kg(-1)). In conclusion, the results confirm peripherally-mediated anti-inflammatory and analgesic activities for V. condensata leaves and suggest that these are influenced by the harvesting season. N-oxides alkaloids as well as vernonioside play important roles in determining this activity262350358CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFundacao Araucari

Talaroxanthone, a Novel Xanthone Dimer from the Endophytic Fungus Talaromyces

DgCr22.1b, an endophytic isolate of Talaromyces sp., was collected in the Amazonian Rainforest from the medicinal plant Duguetia stelechantha. From the fractionation of the methanolic mycelial extract, a new xanthone dimer talaroxanthone was isolated. The structure of this new compound was elucidated based on spectroscopic analyses including 2D nuclear magnetic resonance (NMR) experiments