Significant Increase in Antibody Titers after the 3rd Booster Dose of the Pfizer-BioNTech mRNA COVID-19 Vaccine in Healthcare Workers in Greece.

The aim of our study was to assess the immunogenicity of the third dose of the BNT162b2 mRNA COVID-19 vaccine (Comirnaty) in a cohort of 129 health-care workers in Greece whose anti-S1 RBD IgG titers were monitored over the course of nine months. Titers were measured for each participant just before the third dose (nine months after the second dose) and also one month after the third dose. Of the 129 participants, 19 had been previously infected before starting the vaccination scheme. The SARS-CoV-2 IgG II Quant assay on the Architect System was employed to longitudinally assess the titers of IgG against the receptor-binding domain of the S1 subunit of the spike protein (anti-S1 RBD). Boosters raised Geometric Mean Concentrations (GMCs) by a factor of approximately 47 relative to levels at 9 months and by a factor of approximately 23 relative to levels at 6 months. The immune response one month after the third dose was significantly higher than the response achieved one month after the second dose (p = 0.008). In conclusion, our findings verify the potent immunogenicity elicited by the third dose in all age and prior COVID-19 status groups, suggesting that the timely administration of the third (booster) dose maximizes the immunogenic potential of the vaccine

Field Pea in European Cropping Systems: Adaptability, Biological Nitrogen Fixation and Cultivation Practices

This article provides an overview of the production and use of field pea in European farming systems. Pea is cultivated in Europe for both human consumption and for animal feeding. For food, pea is consumed as dry seeds, green pods or green seeds (fresh, canned or frozen). Field pea is also used for animal feed. Pea production has declined in the region; however, interest in pea cultivation has recently revived. Pea production provides several agronomic advantages in the Mediterranean region supporting more sustainable cropping systems and reduced nitrogen fertiliser use. Furthermore, peas for animal feed partly substitute for the increasingly expensive imported soybean. In addition to describing the current situation of pea cultivation and the future perspectives, this chapter reports on the adaptability of pea in Europe, cropping techniques with emphasis on modern farming practices and varieties that make their cultivation more profitable and more attractive to growers, and the cropping systems that are commonly used for field pea production. The currently applied cropping practices in the region, including rotation, soil tillage practices, fertilisation, sowing and crop density, weed, pest and disease management, irrigation and harvesting, are outlined

Effect of N and P Fertilization on Weed Flora of Maize (Zea Mays L.) Crop

From May to September 2016, a corn hybrid was sown in a field in central Greece. The experiment was laid out in a randomized complete block design with six replications and five fertilization treatments, namely NutriSphere-N Nitrogen Fertilizer (75%), NutriSphere-N Nitrogen Fertilizer (100%), AVAIL Phosphorus Fertilizer (75%), AVAIL Phosphorus Fertilizer (100%) and unfertilized (control). Data analysis confirmed that the different type of fertilization has a significant effect on the composition of weed flora. In particular, the results of the present study indicated significant differences between the fertilization treatments concerning density and diversity of weeds. Furthermore, weed diversity in the control treatment was highest, while it was lower in plots receiving application of N and P. However, total weed dry mass was lower in plots receiving no fertilizer and P fertilization, and highest in plots receiving N. Differences in terms of weed growth between the two types of fertilization could be attributed to differences revealed in the composition of the weed flora.

Efficacy and Selectivity of Pre- and Post-emergence Herbicides in Chia (Salvia hispanica L.) under Mediterranean Semi-arid Conditions

In the present study, the selectivity and efficacy of several post and pre-emergence herbicides in chia (Salvia hispanica L.) were examined. Field experiments were conducted at two locations with different soil types and environmental conditions. Our results showed that the pre-emergence application of pendimethalin, oxyfluorfen and linuron reduced total weed density by 71-74%, 74-82% and 53-55%, respectively. Despite their high effectiveness, the above-mentioned herbicides had a negative effect on plant density and biomass yield. In addition, herbicides bentazon and fluazifop-p-butyl which applied post-emergence did not affect significantly plant height and biomass yield. In conclusion, the herbicides linuron, pendimethalin and oyxfluorfen do not seem to be a viable option for weed control in chia crop, whereas the post-emergence applied herbicides did not affect significantly plants’ growth. Further evaluation of chia tolerance to herbicides is needed under different application rates in order to make safe suggestions for chemical control of weeds

Effect of Different Types of Fertilization on Vigna unguiculata subsp. sesquipedalis Crop

A field experiment was conducted at Pylos, Greece to compare the effect of different types of fertilization on yardlong bean (Vigna unguiculata subsp. sesquipedalis) crop. The conventional treatment plots were fertilized with an inorganic fertilizer, whereas the organic treatments plots received organic compost. Data analysis confirmed no significant correlation between plant height and type of fertilization, but there was significant correlation between length of pods and type of fertilization. In particular, morphological characteristics of yardlong bean were enhanced by inorganic fertilization in comparison with the organic fertilization.  Organic farming increased significantly the number on root nodules in comparison with conventional farming

Spa diversity and genetic characterization of t127 methicillin-resistant Staphylococcus aureus in a tertiary Greek hospital

Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) causes severe community and hospital acquired infections. Identification of staphylococcal cassette chromosome mec (SCCmec), multilocus-sequence typing, and sequencing of S. aureus protein A (spa) gene are used for MRSA typing. The aim was to investigate the spa types of MRSA isolates in a tertiary hospital in Greece and analyse the whole genome sequences of two t127 MRSA isolates. Methods: Totally, 39 MRSA isolates collected from July 2019 to June 2020 in "Georgios Gennimatas" General Hospital of Thessaloniki, Greece, were included in the study. Identification and antimicrobial susceptibility testing were performed using VITEK II automated system, and spa typing was performed. A minimum spanning tree was used to display the spa type frequencies and the genetic distances among them. Two t127-MRSA isolates (IM-MRSA and PD-MRSA) were selected for WGS. Results: Six isolates (15.4%) were resistant to mupirocin, 18 (46.2%) to fusidic acid, three (7.7%) to vancomycin and two (5.1%) to teicoplanin. Twenty-two different spa types were detected, with t002, t003, and t422 being the most frequent (5/39, 12.8% each), followed by t1994 (4/39, 10.3%). The isolates presented high genetic diversity and, taking into account the time between hospital admission and sampling, intrahospital spread did not occur. Even the two t127 isolates were assigned to different sequence types, ST9-XII-t127 and ST1-IVa-t127. Plasmids and genes conferring antimicrobial resistance and virulence were also identified. Conclusions: Various spa types were identified and together with the information about the time between hospital admission and sampling supports polyclonal MRSA spread in the hospital excluding a nosocomial infection. WGS provides a more detailed analysis distinguishing even the isolates belonging to the same spa type

Designing a consequentially based study into the online support of pre-service teachers in the UK

This paper reports on the design of a pilot doctoral study into the online support of pre-service teachers. It highlights the significance of a consequential, rather than deontological, perspective in guiding the development of a study's design. The study initially aimed to explore pre-service teachers’ perceptions and use of social media on their school placements by setting up groups on Facebook and Twitter. However, several problems occurred in relation to the recruitment of participants. It became increasingly clear that there was significance in the positionality of the researcher as an “outsider” to the research context and the potential role for gatekeepers in understanding remote research sites. An ethical framework was used to make a more comprehensive analysis of the issues at play, which helped identify ways of proceeding. A redesign of the study followed with a stronger rationale for the way consequential considerations can help address deontological concerns

Pre-Service Teachers’ Use of Social Media for Academic Purposes

This thesis presents the findings of a qualitative study that was conducted in the context of pre-service teachers in Greece. The study explores how pre-service teachers use social media for academic purposes on their own initiative. Different methods were employed for the collection of data, with semi-structured interviews being the main data collection method. The rich data obtained from 36 pre-service teachers allowed for a complex picture of social media use to emerge. The findings suggest that participants used an array of social media to ‘survive’ their teacher education courses and develop their student identities. At the same time, they used social media to prepare for their transition into their future profession. Through social media participants engaged in social capital building with their existing networks and loose assemblages of people that consisted of their peers and future colleagues. The study discusses the benefits derived from this use of social media. The importance of non-active participation and the building of latent ties is also highlighted. Finally, the thesis presents a framework that aims to link the key concepts that informed the study, both deductively and inductively, and forms my contribution to knowledge. The importance of recognising overlapping boundaries between personal and academic purposes when conceptualising the use of social media, and acknowledging participants’ messy realities is stressed. The study contributes to existing literature by providing in-depth qualitative data of the voluntary ways in which participants used social media for both course-related and profession- related purposes. Based on the findings, the thesis presents theoretical and practical implications for initial teacher education institutions, as well as recommendations for future research into pre-service teachers’ social media use