CORRECTING EFFECT OF AQUEOUS EXTRACT FROM DUCKWEED LEMNA MINORFROND ON MORPHOLOGICAL STATE OF THYROID GLAND IN RATS WITH HYPOTHYROIDISM

The present work studied the effect of aqueous extract from duckweed Lemna minor frond on morphological state of the thyroid gland in the experimental hypothyroidism. Healthy albino rats weighing between 120 g and 150 g were used. The animalswere randomly allotted into four groups, each containing ten rats respectively. Three of the groups (II, III and IV) induced with hypothyroidism by 1 % solution of sodium perchlorate with drinking water for 20 days. Control (vehicle) rats were given normal saline. After 20 days hypothyroid groups (III and IV) of rats treated with aqueous extract from duckweed Lemna minor frond at a dose 0.5 ml/100 g of body weight and with Iodomarin 200 (reference drug) at a dose 12 µg/kg daily orally for 21 days. Results obtained from the study showed that the introduction of sodium perchlorate leads to morphological and functional changes in the structure of the thyroid gland in rats, accompanied by sings of hyperplastic tissue proliferation and corresponds with hypofunctional state. It was established that introduction of aqueous extract from duckweed Lemna minor frond has a positive effect on the restructuring of the thyroid gland in rats with hypothyroidism, increasing its functional activity. There is a decrease of proliferative processes by extrafolliсular epithelium and thyrocytes in the follicles. The outer diameter of follicles decreased by 7.65 % (P>0.05), the height and size of thyrocytes in the wall of the follicle were significantly decreased by 58.16 % and 18 41 %, respectively, compared to untreated hypothyroid group. The obtained results have practical significance for the development of drugs with thyroid-stimulating action for preventing or delaying the development of hypothyroidism and its complications

IFLUENCE OF AQUEOUS EXTRACT FROM FEIJOA LEAVES ON THYROID FUNCTION IN RATS WITH EXPERIMENTAL HYPOTHYROIDISM

The present work studied the effect of aqueous extract from Feijoa leaves on thyroid function of rats with experimental hypothyroidism. Healthy albino rats weighing between 120 gand 150 gwere used. The animals were randomly allotted into four groups, each containing eight rats respectively. Three of the groups (II, III and IV), induced with hypothyroidism, were treated by 0.05 % solution of thiamazole with drinking water for 30 days. Control (vehicle) rats were given normal saline. After 13 days hypothyroid groups (III and IV) of rats were treated with aqueous extract from Feijoa leaves at a dose 1.0 ml/100 g of body weight and with Iodomarin 200 (reference drug) at a dose 12 µg/kg daily orally for 21 days. Results obtained from the study showed that the introduction of thiamazole leads to functional changes in the thyroid gland in rats, accompanied by decreasing sings of rectal temperature and level of thyroid hormones. It was found, that treatment with AEFL normalizes serum thyroid hormone levels, increases rectal temperature and reduces the thyroid mass. The investigated extract can be attributed to the regulators of the thyroid hypofunction and is promising for further study of its effectiveness as a thyroid-stimulating agent

MICROBIOLOGICAL STUDY OF A PERSPECTIVE HEPATOPROTECTIVE AGENT BASED ON DRY EXTRACT FROM PRUNUS DOMESTICA FRUITS

One of the main directions of improving the concept of treatment of diseases of the gastrointestinal tract and liver, which is accompanied by dysfunction of the hepatobiliary system and intestines, was the search and development of herbal remedies with hepatoprotective effect with a laxative effect and concomitant prebiotic activity. Aim of the research: to study the antimicrobial and prebiotic properties of the studied phytosubstance obtained from the fruits of Prunus domestica. Materials and methods. The study of the antimicrobial activity of the test sample of plum fruit extract with fibers was performed in vitro by the method of multiple serial dilutions. The reference strains of the following microorganisms were used as the microbiological model: S.aureus-ATCC-25923, E.сoli-ATCC-25922, P. aéruginosa-ATCC-27853, B. subtilis-ATCC-6633, C.albicans-ATCC-885653. The determination of the prebiotic properties of the studied sample was carried out by the method of cultivation of bacteria Bifidobacterium bifidum No. 1 and L. rhamnosus R0011 ND on nutrient media, which included the composition of plum fruit with fibers. Results. Studies on the antimicrobial activity of plum fruit extract with fibers showed that the studied phytosubstance does not reveal antagonistic properties both with respect to gram-positive and gram-negative bacteria, as well as in the case of Candida fungi. The results of studies on the study of the prebiotic properties of the studied extract confirmed the improvement of the main physiological parameters of the culture of bifidobacteria strain Bifidobacterium bifidum No. 1 and the strain lactobacillus L. rhamnosus R0011 ND when the fruit plums with fibers were added to the culture medium. Conclusions. The conducted studies allowed to establish the prebiotic properties of the studied extract of plum fruits with fibers, which proves the promise of its use in complex therapy schemes for gastrointestinal pathology

CORRECTING EFFECT OF AQUEOUS EXTRACT FROM DUCKWEED LEMNA MINORFROND ON MORPHOLOGICAL STATE OF THYROID GLAND IN RATS WITH HYPOTHYROIDISM

The present work studied the effect of aqueous extract from duckweed Lemna minor frond on morphological state of the thyroid gland in the experimental hypothyroidism. Healthy albino rats weighing between 120 g and 150 g were used. The animalswere randomly allotted into four groups, each containing ten rats respectively. Three of the groups (II, III and IV) induced with hypothyroidism by 1 % solution of sodium perchlorate with drinking water for 20 days. Control (vehicle) rats were given normal saline. After 20 days hypothyroid groups (III and IV) of rats treated with aqueous extract from duckweed Lemna minor frond at a dose 0.5 ml/100 g of body weight and with Iodomarin 200 (reference drug) at a dose 12 µg/kg daily orally for 21 days. Results obtained from the study showed that the introduction of sodium perchlorate leads to morphological and functional changes in the structure of the thyroid gland in rats, accompanied by sings of hyperplastic tissue proliferation and corresponds with hypofunctional state. It was established that introduction of aqueous extract from duckweed Lemna minor frond has a positive effect on the restructuring of the thyroid gland in rats with hypothyroidism, increasing its functional activity. There is a decrease of proliferative processes by extrafolliсular epithelium and thyrocytes in the follicles. The outer diameter of follicles decreased by 7.65 % (P>0.05), the height and size of thyrocytes in the wall of the follicle were significantly decreased by 58.16 % and 18 41 %, respectively, compared to untreated hypothyroid group. The obtained results have practical significance for the development of drugs with thyroid-stimulating action for preventing or delaying the development of hypothyroidism and its complications