Rapid identification of early renal damage in asymptomatic hyperuricemia patients based on urine Raman spectroscopy and bioinformatics analysis

Objective: The issue of when to start treatment in patients with hyperuricemia (HUA) without gout and chronic kidney disease (CKD) is both important and controversial. In this study, Raman spectroscopy (RS) was used to analyze urine samples, and key genes expressed differentially CKD were identified using bioinformatics. The biological functions and regulatory pathways of these key genes were preliminarily analyzed, and the relationship between them as well as the heterogeneity of the urine components of HUA was evaluated. This study provides new ideas for the rapid evaluation of renal function in patients with HUA and CKD, while providing an important reference for the new treatment strategy of HUA disease.Methods: A physically examined population in 2021 was recruited as the research subjects. There were 10 cases with normal blood uric acid level and 31 cases with asymptomatic HUA diagnosis. The general clinical data were collected and the urine samples were analyzed by Raman spectroscopy. An identification model was also established by using the multidimensional multivariate method of orthogonal partial least squares discriminant analysis (OPLS-DA) model for statistical analysis of the data, key genes associated with CKD were identified using the Gene Expression Omnibus (GEO) database, and key biological pathways associated with renal function damage in CKD patients with HUA were analyzed.Results: The Raman spectra showed significant differences in the levels of uric acid (640 cm−1), urea, creatinine (1,608, 1,706 cm−1), proteins/amino acids (642, 828, 1,556, 1,585, 1,587, 1,596, 1,603, 1,615 cm−1), and ketone body (1,643 cm−1) (p < 0.05). The top 10 differentially expressed genes (DEGs) associated with CKD (ALB, MYC, IL10, FOS, TOP2A, PLG, REN, FGA, CCNA2, and BUB1) were identified. Compared with the differential peak positions analyzed by the OPLS-DA model, it was found that the peak positions of glutathione, tryptophan and tyrosine may be important markers for the diagnosis and progression of CKD.Conclusion: The progression of CKD was related to the expression of the ALB, MYC, IL10, PLG, REN, and FGA genes. Patients with HUA may have abnormalities in glutathione, tryptophan, tyrosine, and energy metabolism. The application of Raman spectroscopy to analyze urine samples and interpret the heterogeneity of the internal environment of asymptomatic HUA patients can be combined with the OPLS-DA model to mine the massive clinical and biochemical examination information on HUA patients. The results can also provide a reference for identifying the right time for intervention for uric acid as well as assist the early detection of changes in the internal environment of the body. Finally, this approach provides a useful technical supplement for exploring a low-cost, rapid evaluation and improving the timeliness of screening. Precise intervention of abnormal signal levels of internal environment and energy metabolism may be a potential way to delay renal injury in patients with HUA

Key candidate genes and pathways in T lymphoblastic leukemia/lymphoma identified by bioinformatics and serological analyses

T-cell acute lymphoblastic leukemia (T-ALL)/T-cell lymphoblastic lymphoma (T-LBL) is an uncommon but highly aggressive hematological malignancy. It has high recurrence and mortality rates and is challenging to treat. This study conducted bioinformatics analyses, compared genetic expression profiles of healthy controls with patients having T-ALL/T-LBL, and verified the results through serological indicators. Data were acquired from the GSE48558 dataset from Gene Expression Omnibus (GEO). T-ALL patients and normal T cells-related differentially expressed genes (DEGs) were investigated using the online analysis tool GEO2R in GEO, identifying 78 upregulated and 130 downregulated genes. Gene Ontology (GO) and protein-protein interaction (PPI) network analyses of the top 10 DEGs showed enrichment in pathways linked to abnormal mitotic cell cycles, chromosomal instability, dysfunction of inflammatory mediators, and functional defects in T-cells, natural killer (NK) cells, and immune checkpoints. The DEGs were then validated by examining blood indices in samples obtained from patients, comparing the T-ALL/T-LBL group with the control group. Significant differences were observed in the levels of various blood components between T-ALL and T-LBL patients. These components include neutrophils, lymphocyte percentage, hemoglobin (HGB), total protein, globulin, erythropoietin (EPO) levels, thrombin time (TT), D-dimer (DD), and C-reactive protein (CRP). Additionally, there were significant differences in peripheral blood leukocyte count, absolute lymphocyte count, creatinine, cholesterol, low-density lipoprotein, folate, and thrombin times. The genes and pathways associated with T-LBL/T-ALL were identified, and peripheral blood HGB, EPO, TT, DD, and CRP were key molecular markers. This will assist the diagnosis of T-ALL/T-LBL, with applications for differential diagnosis, treatment, and prognosis

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Correlation of the slope of the initial part of P-wave envelope, hypocenter depth and plate boundary

We investigated correlation of the slope of the initial part of P-wave envelope, hypocenter depth and plate boundaries by B-Δ method, which is used to determine epicentral distances in the Japan Meteorological Agency and Japan Railway Company earthquake early warning (EEW) systems. The Tohoku region was chosen as the study region. 19,899 strong motion data for 265 events with magnitudes in the range from 5.0 to 7.6 from KiK-net (Kiban Kyoshin network) had been collected. The coefficient c to investigate is obtained from the linear relation between logB and logΔ. Compared to the hypocenter depth, the coefficients c of events is more likely to decide by the spatial correlation of the plate boundaries. The differences are likely to be due to earthquake characteristics, since deeper events in the subducting slabs the structural effects are likely to be larger than or comparable to those for shallow crust events. Keywords: The slope of the initial part of P-wave envelope, B-Δ Method, Plate boundary, Hypocenter dept

Mechanoresponsive Material of AIE-Active 1,4-Dihydropyrrolo[3,2-b]pyrrole Luminophores Bearing Tetraphenylethylene Group with Rewritable Data Storage

A new tetraphenylethylene (TPE) functionalized 1,4-dihydropyrrolo[3,2-b]pyrrole derivative (APPTPECN) was synthesized with obvious aggregation-induced emission (AIE) active by simple synthetic method. APPTPECN exhibited reversible mechanofluorochromic (MFC) behavior. The powder X-ray diffraction (PXRD) and scanning electron microscopy (SEM) investigations exhibited that the MFC nature is originated through a conversion from the microcrystalline to amorphous phase under the stimulus of external force. The results obtained would be of major help in understanding the MFC mechanism and designing new MFC materials. Compound APPTPECN has the potential possibility to employ in rewritable data storage and is of assistance in the rational design of smart luminescent materials

Seismic hazard assessment of the Three Gorges Project

Seismic monitoring data for the past 50 years in the Three Gorges Reservoir area show that the reservoir head area is a typical weak seismic region with low seismicity before impoundment and that the epicenters were concentrated in the east and west sides of the Zigui Basin, most of which were natural tectonic earthquakes. After impoundment, the seismic activity shifted to the segment between Badong and Zigui along the Yangtze River, mainly within 5 km of the reservoir bank. The seismogenesis was categorized into four types; Karst collapse earthquakes, earthquakes caused by Karst gas explosion, mining tunnel collapse earthquakes, and rock (terrane) slip earthquakes, all of which are related to the lithology, structure, and tectonics of near-surface geological bodies of the area. Compared with the seismicity before impoundment, the seismic frequency increase was remarkable, with most of the magnitudes below Ms2. 0. Therefore, the intensity of the earthquakes remained at a low level. On November 22, 2008, a magnitude 4. 1 earthquake, the largest earthquake recorded since impoundment, occurred in Quyuan Town, Zigui County. The intensity and PGA of reservoir-induced earthquakes are higher than those of tectonic earthquakes with equal magnitude, but the peak intensity of reservoir-induced earthquakes is not likely to go beyond that of the estimated range from earlier studies

Depolymerization of Methylene Linkage in Condensed Lignin with Commercial Zeolite in Water

Lignin, an aromatic polymer, is a crucial component of lignocellulosic biomass and is a significant source of renewable aromatics found in nature. Lignin constitutes up to 30 wt % of lignocellulosic biomass and is generated in excess of 100 million tons per year from global paper and bio-ethanol industries. Unfortunately, most technical lignin produced in this manner is burned as a heat source, without any value-added valorization. The conversion of technical lignin into valuable compounds can significantly increase the profitability of biorefineries. However, the productivity of this process is constrained by the severe lignin condensation that occurs during extraction procedures, resulting in the formation of robust C–C bonds, such as methylene linkages, which lead to monomer yields of only 8–15%. Herein, we present an approach to overcome this limitation by producing well-defined aromatic monomers from condensed lignin fractions. The approach employs commercial zeolite-catalyzed cleavage of methylene linkages with water as both the reacting medium and the reactant. Up to 7–10% of additional aromatic monomers can be obtained from various types of technical lignin, thus increasing the total monomer yields by 59–102%. The findings of this study present a promising avenue for the valorization of technical lignin and offer potential for the development of circular economy

Could preoxidation always promote the subsequent hydroconversion of lignin? Two counterexamples catalyzed by Cu/CuMgAlOx in supercritical ethanol

In this study, two counterexamples of lignin preoxidation-hydroconversion were reported. First, two lignin feedstocks were preoxidized with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) in acetonitrile with various dosages (15%, 30%, and 60%). Then, these preoxidized lignins (HELOs and MWLOs) were hydroconverted in supercritical ethanol catalyzed by Cu/CuMgAlOx. Total yields from HELOs were all higher than those from HEL, indicating the good promotion of DDQ preoxidation on the subsequent hydroconversion of HELOs, especially with the DDQ dosage of 15%. Differently, the promotion effect of DDQ preoxidation on the hydroconversion of MWLOs depended on the DDQ dosage as well as the reaction time. Through the comparison of two counterexamples, this work bursted the myth that preoxidation can always promote the subsequent hydroconversion of lignin, revealed the influence of lignin property, preoxidation degree, and reaction conditions on the subsequent hydroconversion of preoxidized lignin, and presented the new insight into the preoxidation-hydroconversion strategy for lignin