Isolation and identification of mycobacteria responsible for tuberculosis of dromedaries in Algeria

peer reviewe

Comparison of tuberculin skin test and QuantiFERON (R)-TB Gold In-Tube for the diagnosis of childhood tuberculosis

BackgroundTuberculosis (TB) is an important worldwide ongoing health issue. To be able to control TB, one should not only cure active TB but also identify childhood TB patients who have the possibility to develop active disease in the future. The aim of this study was to compare a century-old tuberculin skin test (TST) and QuantiFERON-TB Gold In-Tube (QFT-GIT) test, developed as an alternative to TST and which has been claimed to be superior to TST in several ways, in the diagnosis of childhood TB

Patients with recurrent tuberculosis in Istanbul: reactivation?, reinfection?

28th International Congress of the European-Respiratory-Society (ERS) -- SEP 15-19, 2018 -- Paris, FRANCEWOS: 000455567106407…European Respiratory So

Simple Identification of Mycobacterial Species by Sequence-Specific Multiple Polymerase Chain Reactions

WOS: 000469873300001PubMed ID: 31073733Several species of mycobacteria cause infections in humans. Species identification of clinical isolates of mycobacteria is very important for the decision of treatment and in choosing the appropriate treatment regimen. We have developed a multiplex PCR method that can identify practically all known species of mycobacteria, by determination of single-nucleotide differences at a total of 13 different polymorphic regions in the genes of rRNA and hsp65, in four PCR mixes. To achieve this goal, single-nucleotide differences in these polymorphic regions were used to divide mycobacterial species into two groups, than four, eight, etc., in an algorithmic manner. It was sufficient to reach single species level by evaluating 13 polymorphic regions. Evaluation of the multiplex PCR patterns by observable real-time electrophoresis (ORTE) simplified species identification. This new method may enable easy, rapid, and cost-effective identification of all species of mycobacteria.TUBTAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [107S014 (SBAG-3541)]This work was supported by TUBTAK 107S014 (SBAG-3541) Identification of Mycobacterial Species by Sequence-Specific Polymerase Chain Reaction; for financial aid, I wish to thank TUBTAK