Data_Sugie_and_Suzuki

Figure 2. Species accumulation curves of 6 samples obtained during the KH12-3 cruise. Sample information is provided as follows: sample 1: 5 m, 28°00’ N, 138°00’ E; sample 2: 75 m, 32°19’ N, 133°33’ E; sample 3: 43 m, 33°05’ N, 133°40’ E; sample 4: 75 m, 35°00’ N, 160°00’ E; sample 5: 5 m, 43°31’ N, 160°00’ E; sample 2: 75 m, 15°00’ N, 160°00’ E. Figure 3. Relationships between (a) J′ calculated based on abundance (J′cell) and biomass (J′bio), and (b) 2E calculated based on abundance (2Ecell) and biomass (2Ebio). The dotted lines in (a) and (b) represent the 1:1 line. (c) Boxplot of J′bio/J′cell and 2Ebio/2Ecell. The upper and lower lines of the box represent the 75% and 25% values. The middle line in the box and circle represents the median and average of the ratios. Crosses represent maximum and minimum values. The ratio = 1 (dotted line), has the same meaning as the 1:1 line shown in Fig. 3a and 3b. Figure 4. Rank order of (a) biomass and (b) abundance of diatoms from the North Pacific. (a-1) and (b-1) show the rank order (line, left axis) and corresponding equivalent spherical diameter (ESD: dots, right axis). Histograms (lower panels) represent the ESD distribution depending on the dominancy shown in (a-1) and (b-1); data were assembled (a-2) and (b-2) 10–100%, (a-3) and (b-3) 1–10%, (a-4) and (b-4) 0.1–1%, and (a-5) and (b-5) <0.1% groups

Species accumulation curves of 6 samples obtained during the KH12-3 cruise (see Fig 1).

<p>Sample information is provided as follows: sample 1: 5 m, 28°00’ N, 138°00’ E; sample 2: 75 m, 32°19’ N, 133°33’ E; sample 3: 43 m, 33°05’ N, 133°40’ E; sample 4: 75 m, 35°00’ N, 160°00’ E; sample 5: 5 m, 43°31’ N, 160°00’ E; sample 2: 75 m, 15°00’ N, 160°00’ E.</p

Sampling stations (circles) in the North Pacific with the cruise names.

<p>White circle: R/V <i>Hakuho-maru</i>, KH11-10 cruise; Black circle: R/V <i>Hakuho-maru</i>, KH12-3 cruise; Light gray circle: R/V <i>Tansei-maru</i>, KT12-5 cruise; Dark gray circle: R/V <i>Tansei-maru</i>, KT12-31 cruise. Arrows with a #number represent the sample IDs shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0131454#pone.0131454.g002" target="_blank">Fig 2</a>.</p

Relationships between (a) <i>J′</i> calculated based on abundance (<i>J′</i>_cell) and biomass (<i>J′</i>_bio), and (b) <i>²E</i> calculated based on abundance (<i>²E</i>_cell) and biomass (<i>²E</i>_bio).

<p>The dotted lines in (a) and (b) represent the 1:1 line. (c) Boxplot of <i>J′</i>_bio/<i>J′</i>_cell and ^<i>2</i><i>E</i>_bio/^<i>2</i><i>E</i>_cell. The upper and lower lines of the box represent the 75% and 25% values. The middle line in the box and circle represents the median and average of the ratios. Crosses represent maximum and minimum values. The ratio = 1 (dotted line), has the same meaning as the 1:1 line shown in Fig 3a and 3b.</p

Histograms represent the ESD distribution based on the relative (a) biomass or (b) abundance shown in Fig 4.

<p>Pooled data were assembled into (a-1) and (b-1) 1–0.1, (a-2) and (b-2) 0.1–0.01, (a-3) and (b-3) 0.01–0.001, and (a-4) and (b-4) <0.001 of relative biomass or abundance.</p

(a) Biomass-ESD and (b) abundance-ESD spectra of diatoms in the North Pacific.

<p>Scattered diagrams (gray dots) represent the relationships between relative (a) biomass or (b) abundance and ESD of pooled samples. Boxplot represents the (a) biomass and (b) abundance of the each size class, established in an octave (log₂) scale. Black circle, box, bars, and crosses represent the mean values of each group, ranges for 25–75% of data, ranges for 1–99% of data, and outliers, respectively.</p

Statistical results of the range of size (width parameter of the Gauss distribution: w, μm) and the mean cell size in the diatom community (m: μm) fitted by the Gaussian function shown in Fig 5.

<p>Data represent the mean ± 1SE of regression. <i>H′</i>_size represents the size diversity shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0131454#pone.0131454.g005" target="_blank">Fig 5</a>.</p><p>Statistical results of the range of size (width parameter of the Gauss distribution: w, μm) and the mean cell size in the diatom community (m: μm) fitted by the Gaussian function shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0131454#pone.0131454.g005" target="_blank">Fig 5</a>.</p

Size-fractionated Chl <i>a</i> and <i>F</i>_v/<i>F</i>_m parameters (value ± 1 SD, n = 3, except for the initial: n = 4) at the initial (day 0) or end (day 3) of the incubation.

<p>Size-fractionated Chl <i>a</i> and <i>F</i>_v/<i>F</i>_m parameters (value ± 1 SD, n = 3, except for the initial: n = 4) at the initial (day 0) or end (day 3) of the incubation.</p

Principal component analysis (PCA) ordination plots incorporating relative contribution of sequence reads for <i>p</i>CO₂ treatments in the DNA and cDNA libraries on day 3.

<p>Each sample is represented as the library type followed by the <i>p</i>CO₂ treatments (i.e., DNA180 indicates the DNA sample from the 180 μatm CO₂ treatment).</p

Relationship between fucoxanthin (Fuco) concentration and diatom-specific <i>rbcL</i> copy number (y = 6.29×10⁸x − 1.90×10⁸, <i>R</i>² = 0.910, <i>p</i> < 0.001, n = 25).

<p>Relationship between fucoxanthin (Fuco) concentration and diatom-specific <i>rbcL</i> copy number (y = 6.29×10⁸x − 1.90×10⁸, <i>R</i>² = 0.910, <i>p</i> < 0.001, n = 25).</p