The “resurrection method” for modification of specific proteins in higher plants

AbstractWe describe a new method designated “the resurrection method” by which a modified protein is expressed in higher plants in place of the original protein. The modified gene constructed by introducing synonymous codon substitutions throughout the original gene to prevent the sequence-specific degradation of its mRNA during RNA silencing is expressed while the expression of the original gene is suppressed. Here, we report the successful alteration of the biochemical properties of green fluorescent protein expressed in transgenic Nicotiana benthamiana, suggesting that this method could be useful for gene control in living plants

Antipredator behavior of newts (Cynops pyrrhogaster) against snakes

Newts and salamanders show remarkable diversity in antipredator behavior, developed to enhance their chemical defenses and/or aposematism. The present study reports on the antipredator behavior of newts (Cynops pyrrhogaster) in response to snakes. Newts displayed a significant amount of tail-wagging and tail-undulation in response to a contact stimulus from the snake’s tongue, which is a snake-specific predator stimulus, as compared to a control stimulus (behavioral scores: tongue, 1.05 ± 0.41; control, 0.15 ± 0.15). Newts that were kept in warm temperature conditions, 20°C (at which snakes are active in nature), performed tail displays more frequently than newts kept in low-temperature conditions, 4°C (at which snakes are inactive in nature). Our results suggest that the tail displays of C. pyrrhogaster could function as an antipredator defense; they direct a snake’s attention to its tail to prevent the snake from attacking more vulnerable body parts. We also discussed the reason for inter-populational variation in the tendency of newts to perform tail displays

Discovery of X-Ray-Emitting O-Ne-Mg-Rich Ejecta in the Galactic Supernova Remnant Puppis A

We report on the discovery of X-ray--emitting O-Ne-Mg-rich ejecta in the middle-aged Galactic O-rich supernova remnant Puppis A with Chandra and XMM-Newton. We use line ratios to identify a low-ionization filament running parallel to the northeastern edge of the remnant that requires supersolar abundances, particularly for O, Ne, and Mg, which we interpret to be from O-Ne-Mg-rich ejecta. Abundance ratios of Ne/O, Mg/O, and Fe/O are measured to be ~2, ~2, and <0.3 times the solar values. Our spatially-resolved spectral analysis from the northeastern rim to the western rim otherwise reveals sub-solar abundances consistent with those in the interstellar medium. The filament is coincident with several optically emitting O-rich knots with high velocities. If these are physically related, the filament would be a peculiar fragment of ejecta. On the other hand, the morphology of the filament suggests that it may trace ejecta heated by a shock reflected strongly off the dense ambient clouds near the northeastern rim.Comment: Published onlin

A Suzaku Study of Ejecta Structure and Origin of Hard X-ray Emission in the Supernova Remnant G156.2+5.7

We report an X-ray study of the evolved Galactic supernova remnant (SNR) G156.2+5.7 based on six pointing observations with Suzaku. The remnant's large extent (100\arcmin in diameter) allows us to investigate its radial structure in the northwestern and eastern directions from the apparent center. The X-ray spectra were well fit with a two-component non-equilibrium ionization model representing the swept-up interstellar medium (ISM) and the metal-rich ejecta. We found prominent central concentrations of Si, S and Fe from the ejecta component; the lighter elements of O, Ne and Mg were distributed more uniformly. The temperature of the ISM component suggests a slow shock (610-960 km s$^{-1}$), hence the remnant's age is estimated to be 7,000-15,000 yr, assuming its distance to be $\sim$1.1 kpc. G156.2+5.7 has also been thought to emit hard, non-thermal X-rays, despite being considerably older than any other such remnant. In response to a recent discovery of a background cluster of galaxies (2XMM J045637.2+522411), we carefully excluded its contribution, and reexamined the origin of the hard X-ray emission. We found that the residual hard X-ray emission is consistent with the expected level of the cosmic X-ray background. Thus, no robust evidence for the non-thermal emission was obtained from G156.2+5.7. These results are consistent with the picture of an evolved SNR.Comment: 10 pages 8 figures, accepted for PAS

X-ray ejecta kinematics of the Galactic core-collapse supernova remnant G292.0+1.8

We report on the results from the analysis of our 114 ks Chandra HETGS observation of the Galactic core-collapse supernova remnant G292.0+1.8. To probe the 3D structure of the clumpy X-ray emitting ejecta material in this remnant, we measured Doppler shifts in emission lines from metal-rich ejecta knots projected at different radial distances from the expansion center. We estimate radial velocities of ejecta knots in the range of -2300 <~ v_r <~ 1400 km s^-1. The distribution of ejecta knots in velocity vs. projected-radius space suggests an expanding ejecta shell with a projected angular thickness of ~90" (corresponding to ~3 pc at d = 6 kpc). Based on this geometrical distribution of the ejecta knots, we estimate the location of the reverse shock approximately at the distance of ~4 pc from the center of the supernova remnant, putting it in close proximity to the outer boundary of the radio pulsar wind nebula. Based on our observed remnant dynamics and the standard explosion energy of 10^51 erg, we estimate the total ejecta mass to be <~ 8 M_sun, and we propose an upper limit of <~ 35 M_sun on the progenitor's mass.Comment: 5 figures, accepted by Ap

An Apparent Hard X-ray Decline of CH Cygni

CH Cygni is a symbiotic star consisting of an M giant and an accreting white dwarf, which is known to be a highly variable X-ray source with a complex, two-component, spectra. Here we report on two Suzaku observations of CH Cyg, taken in 2006 January and May, during which the system was seen to be in a soft X-ray bright, hard X-ray faint state. Based on the extraordinary strength of the 6.4 keV fluorescent Fe K-alpha line, we show that the hard X-rays observed with Suzaku are dominated by scattering.Comment: 8 pages, 4 figures, accepted by Publications of the Astronomical Society of Japa

Enhancement of plastic anisotropy and drastic increase in yield stress of Mg-Li single crystals by Al-addition followed by quenching

Strong orientation dependence of yield stress was found in a body-centered cubic (bcc)-structured Mg-Li alloy single crystal by adding 5 at.% of Al combined with rapid quenching, which was negligible in Mg-Li binary single crystals. Furthermore, the addition of 5 at.% of Al combined with rapid quenching caused an extreme increase in yield stress up to ~470 MPa; this compares to ~50 MPa in a Mg-Li binary crystal. Increased valence-electron to atom ratio and development of chemical modulation in the alloy by Al-addition are probable causes of the enhancement of plastic anisotropy and the drastic increase in yield stress, respectively.Hagihara K., Mori K., Nakano T.. Enhancement of plastic anisotropy and drastic increase in yield stress of Mg-Li single crystals by Al-addition followed by quenching. Scripta Materialia, 172, 93. https://doi.org/10.1016/j.scriptamat.2019.07.012