2,542 research outputs found
489 ANAPHYLATOXINE RECEPTORS AND COMPLEMENT REGULATORY PROTEINS IN TENDON: INTERRELATION WITH IMMUNOREGULATORY CYTOKINES
Self-organized transition to coherent activity in disordered media
Synchronized oscillations are of critical functional importance in many
biological systems. We show that such oscillations can arise without
centralized coordination in a disordered system of electrically coupled
excitable and passive cells. Increasing the coupling strength results in waves
that lead to coherent periodic activity, exhibiting cluster, local and global
synchronization under different conditions. Our results may explain the
self-organized transition in a pregnant uterus from transient, localized
activity initially to system-wide coherent excitations just before delivery.Comment: 5 pages, 4 figure
Hybrid simulations of lateral diffusion in fluctuating membranes
In this paper we introduce a novel method to simulate lateral diffusion of
inclusions in a fluctuating membrane. The regarded systems are governed by two
dynamic processes: the height fluctuations of the membrane and the diffusion of
the inclusion along the membrane. While membrane fluctuations can be expressed
in terms of a dynamic equation which follows from the Helfrich Hamiltonian, the
dynamics of the diffusing particle is described by a Langevin or Smoluchowski
equation. In the latter equations, the curvature of the surface needs to be
accounted for, which makes particle diffusion a function of membrane
fluctuations. In our scheme these coupled dynamic equations, the membrane
equation and the Langevin equation for the particle, are numerically integrated
to simulate diffusion in a membrane. The simulations are used to study the
ratio of the diffusion coefficient projected on a flat plane and the
intramembrane diffusion coefficient for the case of free diffusion. We compare
our results with recent analytical results that employ a preaveraging
approximation and analyze the validity of this approximation. A detailed
simulation study of the relevant correlation functions reveals a surprisingly
large range where the approximation is applicable.Comment: 12 pages, 9 figures, accepted for publication in Phys. Rev.
Reproductive isolation in the Aegean Ophrys omegaifera complex (Orchidaceae)
The orchid genus Ophrys operates a system of sexual deception by which high specificity of pollination is attained. Reproductive isolation in Ophrys mainly rests upon prezygotic isolation mechanisms. The level of genetic separateness of Ophrys taxa with different pollinators is therefore likely determined by the fidelity of pollinators. The present study employs genetic fingerprinting to investigate this in the east Aegean Ophrys omegaifera s.l. complex, also including O. dryis, a west Mediterranean species of this complex. Ophrys fleischmannii, O. basilissa, and the west Mediterranean O. dyris, are found to be well-separated genetic entities whereas O. omegaifera s.str. and the putative hybrid taxon, O. sitiaca, are found to be genetically inseparable across their entire range of co-occurrence. This suggests that specific pollinators have high enough fidelity to act as effective isolating factors in east Aegean O. omegaifera s.l. as a whole, but that the situation in the species pair of O. sitiaca and O. omegaifera is likely to be more comple
Calpain cleavage of Junctophilin-2 generates a spectrum of calcium-dependent cleavage products and DNA-rich NT1-fragment domains in cardiomyocytes
Calpains are calcium-activated neutral proteases involved in the regulation of key signaling pathways. Junctophilin-2 (JP2) is a Calpain-specific proteolytic target and essential structural protein inside Ca2+ release units required for excitation-contraction coupling in cardiomyocytes. While downregulation of JP2 by Calpain cleavage in heart failure has been reported, the precise molecular identity of the Calpain cleavage sites and the (patho-)physiological roles of the JP2 proteolytic products remain controversial. We systematically analyzed the JP2 cleavage fragments as function of Calpain-1 versus Calpain-2 proteolytic activities, revealing that both Calpain isoforms preferentially cleave mouse JP2 at R565, but subsequently at three additional secondary Calpain cleavage sites. Moreover, we identified the Calpain-specific primary cleavage products for the first time in human iPSC-derived cardiomyocytes. Knockout of RyR2 in hiPSC-cardiomyocytes destabilized JP2 resulting in an increase of the Calpain-specific cleavage fragments. The primary N-terminal cleavage product NT1 accumulated in the nucleus of mouse and human cardiomyocytes in a Ca2+-dependent manner, closely associated with euchromatic chromosomal regions, where NT1 is proposed to function as a cardio-protective transcriptional regulator in heart failure. Taken together, our data suggest that stabilizing NT1 by preventing secondary cleavage events by Calpain and other proteases could be an important therapeutic target for future studies
Noncollinear magnetic ordering in small Chromium Clusters
We investigate noncollinear effects in antiferromagnetically coupled clusters
using the general, rotationally invariant form of local spin-density theory.
The coupling to the electronic degrees of freedom is treated with relativistic
non-local pseudopotentials and the ionic structure is optimized by Monte-Carlo
techniques. We find that small chromium clusters (N \le 13) strongly favor
noncollinear configurations of their local magnetic moments due to frustration.
This effect is associated with a significantly lower total magnetization of the
noncollinear ground states, ameliorating the disagreement between Stern-Gerlach
measurements and previous collinear calculations for Cr_{12} and Cr_{13}. Our
results further suggest that the trend to noncollinear configurations might be
a feature common to most antiferromagnetic clusters.Comment: 9 pages, RevTeX plus .eps/.ps figure
Coronal Diagnostics from Narrowband Images around 30.4 nm
Images taken in the band centered at 30.4 nm are routinely used to map the
radiance of the He II Ly alpha line on the solar disk. That line is one of the
strongest, if not the strongest, line in the EUV observed in the solar
spectrum, and one of the few lines in that wavelength range providing
information on the upper chromosphere or lower transition region. However, when
observing the off-limb corona the contribution from the nearby Si XI 30.3 nm
line can become significant. In this work we aim at estimating the relative
contribution of those two lines in the solar corona around the minimum of solar
activity. We combine measurements from CDS taken in August 2008 with
temperature and density profiles from semiempirical models of the corona to
compute the radiances of the two lines, and of other representative coronal
lines (e.g., Mg X 62.5 nm, Si XII 52.1 nm). Considering both diagnosed
quantities from line ratios (temperatures and densities) and line radiances in
absolute units, we obtain a good overall match between observations and models.
We find that the Si XI line dominates the He II line from just above the limb
up to ~2 R_Sun in streamers, while its contribution to narrowband imaging in
the 30.4 nm band is expected to become smaller, even negligible in the corona
beyond ~2 - 3 R_Sun, the precise value being strongly dependent on the coronal
temperature profile.Comment: 26 pages, 11 figures; to be published in: Solar Physic
Curvature-coupling dependence of membrane protein diffusion coefficients
We consider the lateral diffusion of a protein interacting with the curvature
of the membrane. The interaction energy is minimized if the particle is at a
membrane position with a certain curvature that agrees with the spontaneous
curvature of the particle. We employ stochastic simulations that take into
account both the thermal fluctuations of the membrane and the diffusive
behavior of the particle. In this study we neglect the influence of the
particle on the membrane dynamics, thus the membrane dynamics agrees with that
of a freely fluctuating membrane. Overall, we find that this curvature-coupling
substantially enhances the diffusion coefficient. We compare the ratio of the
projected or measured diffusion coefficient and the free intramembrane
diffusion coefficient, which is a parameter of the simulations, with analytical
results that rely on several approximations. We find that the simulations
always lead to a somewhat smaller diffusion coefficient than our analytical
approach. A detailed study of the correlations of the forces acting on the
particle indicates that the diffusing inclusion tries to follow favorable
positions on the membrane, such that forces along the trajectory are on average
smaller than they would be for random particle positions.Comment: 16 pages, 8 figure
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