Administration of isoferulic acid improved the survival rate of lethal influenza virus pneumonia in mice.

BACKGROUND: Isoferulic acid (IFA) is a main active ingredient of the rhizoma of Cimicifuga beracleifolia, which is used frequently in Japanese traditional medicine as an anti-inflammatory drug. It has been revealed that IFA inhibits the production of macrophage inflammatory protein-2 (MIP-2), which is a murine counterpart of the chemokine family that may contribute to the pathogenesis of inflammatory diseases through the chemotactic activity for inflammatory and immune effector cells. AIM OF THE STUDY: In this study, we investigated the therapeutic effect of IFA on the progression of lethal influenza virus pneumonia in mice by comparison with that of dexamethasone (DX), a potent inhibitor for various inflammatory cytokines including MIP-2. METHODS: Mice were infected by intranasal inoculation of influenza virus under ether anesthesia. The IFA or DX was given by oral administration once daily for 4 days after infection. After infection, the survival rate and the change in body weight were daily monitored. RESULTS: IFA administration markedly improved the survival rate and body weight loss of influenza virus-infected mice in a suitable dose range (0.5 mg/day). However, DX administration did not show a beneficial effect at any dose. CONCLUSION: These data suggested that IFA is a novel tool not only for the intervention therapy, but also for the studies on the pathogenesis of influenza virus-induced pneumonia

Development and some applications of enzyme-linked immunosorbent assay system for murine macrophage inflammatory protein-2 (MIP-2)

We attempted to establish an enzyme-linked immunosorbent assay (ELISA) system by preparation of recombinant murine MIP-2 and its rabbit antibodies. A fusion construct of MIP-2 to protein A was used to enable easy purification as well as the generation of a sufficiently large antibody response. The specificity of antibody was confirmed by Western blotting analysis of 20-h conditioned medium from lipopolysaccharide (LPS)-stimulated RAW264.7 cells, a murine macrophage cell line; antibody gave a single band with a molecular weight of approximately 6000, which is identical to that of murine MIP-2 reported previously. Biotin–streptavidin sandwich ELISA could detect quantitatively MIP-2 at concentration range of 20 to 1000 pg/ml. In some applications of this ELISA system, time-related production of MIP-2 and inhibitory effect of dexamethasone on its production have been demonstrated in LPS-stimulated RAW264.7 cells. Thus, ELISA system established in this study is considered to be a useful tool to study MIP-2 response in various inflammation models in mice

Survey observations of emission-line stars in the Orion region

Survey observations were conducted for H alpha-emission stars in the Orion region using the Kiso Schmidt telescope and partly the CTIO Curtis Schmidt telescope. In the area of about 25 square degrees, a total of 236 H alpha-emission objects, mostly supposed to be T Tau type stars, have been detected among which 155 are new ones including 6 non-stellar objects. Celestial coordinates and V-magnitude are measured for the detected objects. Eye estimation of the H alpha-emission intensity is also made at three epochs in a time span of about two years, where notable variation of H alpha intensity was found in 68 out of 236 objects. Besides a remarkable concentration along the northern dark cloud complex, a loose concentration is noticed near the Orion Belt region, fairly well coinciding with the distribution of the Orion OBIb association members. A comparison with the Av-map is also made to see the relationship between the distribution of emission-line objects and that of interstellar dust

Out-of-plane dielectric constant and insulator-superconductor transition in Bi_2Sr_2Dy_{1-x}Er_xCu_2O_8 single crystals

The out-of-plane dielectric constant of the parent insulator of the high-temperature superconductor Bi_2Sr_2(Dy,Er)Cu_2O_8 was measured and analysed from 80 to 300 K in the frequency range of 10^6-10^9 Hz. All the samples were found to show a fairly large value of 10-60, implying some kind of charge inhomogeneity in the CuO_2 plane. Considering that the superconducting sample Bi_2Sr_2(Ca,Pr)Cu_2O_8 also shows a similar dielectric constant, the charge inhomogeneity plays an important role in the insulator-superconductor transition.Comment: RevTex4 format, 5 pages, 3 figures, submitted to J. Phys. Condens. Ma

Inhibitory effect of ferulic acid and isoferulic acid on the production of macrophage inflammatory protein-2 in response to respiratory syncytial virus infection in RAW264.7 cells.

We investigated the effect of ferulic acid (FA) and isoferulic acid (IFA), which are the main active components of the rhizoma of Cimicifuga heracleifolia (CH), an anti-inflammatory drug used frequently in Japanese traditional medicine, on the production of macrophage inflammatory protein-2 (MIR-2) in a murine macrophage cell line, RAW264.7, in response to respiratory syncytial virus (RSV) infection. Following the exposure of cells to RSV for 20h, the MIP-2 level in condition medium was increased to about 20 ng/ml, although this level in mock-infected cells was negligible. In the presence of either FA or IFA, RSV-infected cells reduced MIP-2 production in a dose-dependent manner. These data suggest that FA and IFA might be responsible, at least in part, for the anti-inflammatory drug effect of CH extract through the inhibition of MIP-2 production

Alkane-degrading bacteria and heavy metals from the Nakhodka oil spill-polluted seashores in the Sea of Japan after five years of bioremediation

Graduate School of Natural Science and Technology, Kanazawa UniversityDepartment of Earth Sciences, Faculty of Science, Kanazawa UniversityDepartment of Earth Sciences, Faculty of Science, Kanazawa UniversityOcean Research Institute, University of TokyoThis paper describes the isolation of alkane-degrading bacteria from the Nakhodka oil spill-polluted seashores in the Sea of Japan. Seven representative strains were identified using 16S rDNA sequence analysis as Bacillus spp., Pseudomonas sp., and Paracoccus spp. All bacterial strains showed their ability to grow well on aliphatic hydrocarbons, but not on aromatic hydrocarbons. In addition, elemental levels in heavy oil showed wide ranges in all the heavy oil samples consisting of Si, S, Ti, Cr, Mn, Fe, Co, Ni, Cu, Zn, and Pb. Compounds of Si, S, and Cr were observed at high levels, while those of Ti, Mn, Fe, Co, Ni, Cu, Zn, and Pb were observed at low levels. Of all heavy metals, Co appeared to be toxic for all bacterial growth at concentrations of >1 ppm, while the presence of Ti, Cr, and Cu at 0.01 to 10 ppm were found not to inhibit growth of all bacterial strains. It is suspected that the presence of heavy metals may have a significant effect on the composition of the bacterial community, (i.e., alkane-degrading bacterial isolates), as well as on the biodegradative processes of the Nakhodka oil spill during the 5-year bioremediation