Efektivitas Model dan Ketinggian Perangkap dalam Menangkap Hama Lalat Buah Jantan, Bactrocera Spp

. Penelitian ini bertujuan mengetahui model dan ketinggian perangkap yang efektif dalam menangkap lalat buah, Bactrocera spp. Penelitian ini dilakukan di kebun petani di Kenagarian Kacang pada pertanaman polikultur dan Alahan Panjang pada pertanaman monokultur, Solok mulai Maret 2003-Desember 2004. Model alat perangkap yang digunakan dalam penelitian ini yaitu perangkap bekas botol air mineral, modifikasi gipsy moth, modifikasi perangkap steiner, perangkap delta, dan perangkap McPhail. Masing-masing perangkap diberi umpan dengan 0,5 ml metil eugenol atau cue lure yang diteteskan pada sepotong kapas dan digantungkan di dalam perangkap. Untuk mengetahui ketinggian perangkap yang efektif untuk menangkap lalat buah, penelitian dilakukan dengan memasang perangkap dari botol air mineral pada ketinggian 0,5, 1, 1,5, 2, dan 2,5 m dari permukaan tanah. Hasil penelitian menunjukkan bahwa jumlah lalat buah yang tertangkap per perangkap/hari pada masing-masing jenis perangkap yang digunakan berbeda nyata. Lalat buah yang paling banyak tertangkap per perangkap/hari adalah pada perangkap McPhail yaitu 52 ekor dan yang paling sedikit diperoleh pada perangkap gipsy moth yaitu 6 ekor/perangkap/hari. Ketinggian perangkap terlihat berbeda nyata, di mana lalat buah yang paling banyak tertangkap adalah pada perangkap dengan ketinggian 1,5 m dari permukaan tanah. Penggabungan antara model perangkap dengan atraktan sintetis, seperti metil eugenol atau cue lure meningkatkan kemampuan tidak hanya sebagai perangkap lalat buah yang potentsial tetapi juga sebagai alat monitoring hama lalat buah

Distribusi Spesies Lalat Buah di Sumatera Barat dan Riau

. Penelitian ini bertujuan mengetahui distribusi dan diversitas spesies lalat buah di Sumatera Barat dan Pulau Kundur Kabupaten Karimun, Riau. Penelitian dilakukan dengan metode survei mulai bulan Juni 2003 – Desember 2004. Lalat buah ditangkap menggunakan perangkap yang terbuat dari botol bekas air mineral yang di dalamnya digantungkan kapas yang telah dibasahi dengan metil eugenol dan cue lure. Lalat buah hasil tangkapan dibawa ke Laboratorium Proteksi Balai Penelitian Tanaman Buah Tropika Solok untuk diidentifikasi menggunakan kunci identifikasi elektronik Cabikey. Hasil penelitian menunjukkan bahwa dari semua lokasi penelitian diperoleh 45 spesies lalat buah (2 spesies belum teridentifikasi). Tiga spesies lalat buah ditemukan di semua lokasi penelitian, yaitu B. albistrigata, B. carambolae, dan B. papayae. Keragaman spesies lalat buah paling tinggi ditemukan di Kabupaten Solok, Sumatera Barat dan Pulau Kundur, berturut-turut ditemukan 30 spesies dan 25 spesies lalat buah. Hasil penelitian ini bermanfaat sebagai informasi terutama bagi karantina sebagai dasar untuk memperketat aturan mengenai keluar masuknya buah-buahan dari dan ke suatu daerah sehingga spesies-spesies yang ada di daerah tertentu terutama daerah yang mempunyai diversitas spesies tinggi tidak masuk ke daerah lain

Vascularity and perfusion of human gliomas xenografted in the athymic nude mouse.

The vascularisation and perfusion of seven subcutaneously xenografted human glioma lines established from surgical specimens has been analysed using an anti-collagen type IV antibody to visualise the vascular walls in combination with a perfusion marker (Hoechst 33342). A computer-based digital image processing system was employed for quantitative analysis of the parameters. The vascular architecture of individual tumours belonging to the same tumour line showed a consistent similarity, while substantial differences occurred between the various tumour lines derived from different patients. Despite the presence of a large inter-tumour variation in vascular area as a proportion of the tumour area, this vascular parameter clearly showed tumour line-specific characteristics. The perfused fraction of the tumour vessels also showed a large inter-tumour variation for all tumour lines ranging from 20% to 85%, but the majority of tumours of all lines had perfusion fractions of more than 55%. Despite large variation, the perfused vascular area as a proportion of the tumour cross-sectional area exhibited clear tumour line-specific tendencies. These observations suggest that consistent differences in vascular parameters are present between glioma xenograft lines, although the tumour lines all originated from histologically similar human high-grade gliomas. These differences may have important consequences for treatment and clinical behaviour of this type of tumour

Multiparameter analysis of vasculature, perfusion and proliferation in human tumour xenografts.

A method is presented in this report for concurrent analysis of vascular architecture, blood perfusion and proliferation characteristics in whole-tumour cross-sections of human larynx carcinoma and glioblastoma xenografts. Tumours were implanted subcutaneously in nude mice. After i.v. injection with Hoechst 33342 and bromodeoxyuridine (BrdUrd) as perfusion and proliferation markers, animals were killed. An antiendothelial antibody (9F1) was used to delineate vascular structures. Cross-sections were analysed by a multistep immune staining and a computer-controlled microscope scanning method. Each tumour section was stained and scanned four times (Hoechst, 9F1, BrdUrd and Fast Blue for all nuclei). When these images were combined, vasculature, perfusion and proliferation parameters were analysed. The labelling index (LI) was defined as the ratio of the BrdUrd-labelled area to the total nuclear area. The LI based on manual counting and the LI calculated by flow cytometry (FCM) were in good agreement with the LI based on surface analysis. LI decreased at increasing distance from its nearest vessel. In the vicinity of perfused vessels, the LI was 30-70% higher than near non-perfused vessels. This method shows that both vasculature/perfusion and proliferation characteristics can be measured in the same whole-tumour section in a semiautomatic way. This could be applied in clinical practice to identify combined human tumour characteristics that predict for a favourable response to treatment modifications

In vivo (31)P magnetic resonance spectroscopy and morphometric analysis of the perfused vascular architecture of human glioma xenografts in nude mice.

The relationship between the bioenergetic status of human glioma xenografts in nude mice and morphometric parameters of the perfused vascular architecture was studied using (31)P magnetic resonance spectroscopy (MRS), fluorescence microscopy and two-dimensional digital image analysis. Two tumour lines with a different vascular architecture were used for this study. Intervascular distances and non-perfused area fractions varied greatly between tumours of the same line and tumours of different lines. The inorganic phosphate-nucleoside triphosphate (P(i)/NTP) ratio increased rapidly as mean intervascular distances increased from 100 microm to 300 microm. Two morphometric parameters - the percentage of intervascular distances larger than 200 microm (ivd200) and the non-perfused area fraction at a distance larger than 100 microm from a nearest perfused vessel (area100), - were deduced from these experiments and related to the P(i)/NTP ratio of the whole tumour. It is assumed that an aerobic to anaerobic transition influences the bioenergetic status, i.e. the P(i)/NTP ratio increased linearly with the percentage of ivd200 and the area100

Vascular perfusion and hypoxic areas in RIF-1 tumours after photodynamic therapy.

The influence of photodynamic therapy (PDT) on vascular perfusion and the development of hypoxia was investigated in the murine RIF-1 tumour. Image analysis was used to quantify changes in perfusion and hypoxia at 5 min after interstitial Photofrin-mediated PDT. The fluorescent stain Hoechst 33342 was used as an in vivo marker of functional vascular perfusion and the antibody anti-collagen type IV as a marker of the tumour vasculature. The percentage of total tumour vasculature that was perfused decreased to less than 30% of control values after PDT. For the lower light doses this decrease was more pronounced in the centre of the tumour. The observed reduction in vascular perfusion showed a good linear correlation (r = 0.98) with previously published tumour perfusion data obtained with the 86Rb extraction technique. The image analysis technique provides extra information concerning the localisation of (non)-perfused vessels. To detect hypoxic tumour areas in vivo, an immunohistochemical method was used employing NITP [7-(4'-(2-nitroimidazol-1-yl)-butyl)-theophylline]. A large increase in hypoxic areas was found for PDT-treated tumours. More than half the total tumour area was hypoxic after PDT, compared with < 4% for control tumours. Our studies illustrate the potential of image analysis systems for monitoring the functional consequences of PDT-mediated vascular damage early after treatment. This provides direct confirmation that the perfusion changes lead to tissue hypoxia, which has implications for the combined treatment of PDT with bioreductive drugs

Vascular architecture and hypoxic profiles in human head and neck squamous cell carcinomas

Tumour oxygenation and vasculature are determinants for radiation treatment outcome and prognosis in patients with squamous cell carcinomas of the head and neck. In this study we visualized and quantified these factors which may provide a predictive tool for new treatments. Twenty-one patients with stage III–IV squamous cell carcinomas of the head and neck were intravenously injected with pimonidazole, a bioreductive hypoxic marker. Tumour biopsies were taken 2 h later. Frozen tissue sections were stained for vessels and hypoxia by fluorescent immunohistochemistry. Twenty-two sections of biopsies of different head and neck sites were scanned and analysed with a computerized image analysis system. The hypoxic fractions varied from 0.02 to 0.29 and were independent from T- and N-classification, localization and differentiation grade. No significant correlation between hypoxic fraction and vascular density was observed. As a first attempt to categorize tumours based on their hypoxic profile, three different hypoxia patterns are described. The first category comprised tumours with large hypoxic, but viable, areas at distances even greater than 200 μm from the vessels. The second category showed a typical band-like distribution of hypoxia at an intermediate distance (50–200 μm) from the vessels with necrosis at greater distances. The third category demonstrated hypoxia already within 50 μm from the vessels, suggestive for acute hypoxia. This method of multiparameter analysis proved to be clinically feasible. The information on architectural patterns and the differences that exist between tumours can improve our understanding of the tumour micro-environment and may in the future be of assistance with the selection of (oxygenation modifying) treatment strategies. © 2000 Cancer Research Campaig

Pimonidazole binding in C6 rat brain glioma: relation with lipid droplet detection

Item does not contain fulltextIn C6 rat brain glioma, we have investigated the relation between hypoxia and the presence of lipid droplets in the cytoplasm of viable cells adjacent to necrosis. For this purpose, rats were stereotaxically implanted with C6 cells. Experiments were carried out by the end of the tumour development. A multifluorescence staining protocol combined with digital image analysis was used to quantitatively study the spatial distribution of hypoxic cells (pimonidazole), blood perfusion (Hoechst 33342), total vascular bed (collagen type IV) and lipid droplets (Red Oil) in single frozen sections. All tumours (n=6) showed necrosis, pimonidazole binding and lipid droplets. Pimonidazole binding occurred at a mean distance of 114 microm from perfused vessels mainly around necrosis. Lipid droplets were principally located in the necrotic tissue. Some smaller droplets were also observed in part of the pimonidazole-binding cells surrounding necrosis. Hence, lipid droplets appeared only in hypoxic cells adjacent to necrosis, at an approximate distance of 181 microm from perfused vessels. In conclusion, our results show that severe hypoxic cells accumulated small lipid droplets. However, a 100% colocalisation of hypoxia and lipid droplets does not exist. Thus, lipid droplets cannot be considered as a surrogate marker of hypoxia, but rather of severe, prenecrotic hypoxia