Homeopathic products and homeopathic treatment in veterinary medicine in Republic of Slovenia

Doctors and veterinarians in many countries of the world have an opportunity to select between allopathic or homeopathic drugs. The selection of an appropriate method for medical treatment should be performed from the individual point of view.

Neracionalna potrošnja lekova u veterinarskoj medicini - opasnost po zdravlje životinja i ljudi

The pharmaceutical industry, which allowed the achievements of contemporary medicine, has been extremely developed during the last five decades. At the same time, nonrational use of drugs also increased. Thus usage of many medicines confirmed their effectiveness, but at the same time the reports about unwanted and harmful effects became frequent. Uncontrolled use of antimicrobial drugs in production animals, does not only compromise their health, but also through the food the human health.Tokom poslednjih pet decenija farmaceutska industrija je izrasla u jednu od najmoćnijih industrijskih grana, bez koje se savremena medicina ne može ni zamisliti. Međutim, uporedo sa razvojem farmaceutske industrije sve više je rasla i neracionalna potrošnja lekova, a time i češća pojava neželjenih efekata. Poseban značaj ima neracionalna primena antimikrobnih lekova kod životinja namenjenih za ishranu ljudi, jer može da ugrozi ne samo zdravlje tretiranih životinja, već i ljudi

Preventing ectoparasite infestations reduces glucocorticoid concentrations in the hair of cows – short communication

The aim of this study was to compare hair corticosteroid concentrations in ectoparasiticide-treated (n = 10) and non-treated (n = 12) Holstein cows. Animals in the treated group received cyfluthrin three times, on days 0, 28 and 56 of the experiment. Hair samples were collected from all cows on days 0, 21, 42, 63, and 84 of the experiment for the determination of cortisol and corticosterone concentrations using immunoassays. The respective hair cortisol concentrations in the treated group averaged 209.78, 165.10, 260.78, 177.44 and 183.11 ng/g, while in the non-treated group hair cortisol concentrations averaged 172.58, 243.58, 309.73, 199.75 and 207.09 ng/g. These results indicate that the control of ectoparasites reduced hair cortisol levels in dairy cows. The respective hair corticosterone concentrations in the treated group averaged 19.06, 22.95, 21.95, 21.60 and 24.84 ng/g and in the non-treated group the hair corticosterone concentrations averaged 17.28, 21.94, 34.05, 26.27 and 26.91 ng/g. The results suggest that longterm stress can be estimated better by the determination of hair cortisol rather than corticosterone concentrations

Značaj ispitivanja lekova koji se koriste u veterinarskoj medicini

The use of veterinary medicines in Slovenia is regulated by the Medicinal Products Act, by the Veterinary Practice Act, other legislative acts and some administrative acts. Supervision and coordination over pre-clinical and clinical evaluation is preformed by the Laboratory of Pharmacology and Toxicology which is a part of the Unit for Laboratory and Clinical Testing of Drugs at the National Veterinary Institute, on the basic of the concession. The Laboratory of Pharmacology and Toxicology to cooperate with the clinics and institutes of the Veterinary Faculty in Ljubljana.Upotreba lekova u veterinarskoj medicini u Republici Slovenji regulisana je Zakonom o lekovima, Zakonom o veterinarstvu i podzakonskim aktima iz ove oblasti. Koordinacija i nadzor nad prekliničkim (laboratorijskim) i kliničkim ispitivanjima lekova, koji se koriste u veterinarskoj medicini vrši se u saglasnosti sa Katedrom za farmakologiju i toksikologiju, koja (zajedno sa Veterinarskim nacionalnim institutom), čini jedinstven sistem za Laboratorijsko i kliničko ispitivanje lekova. U tom pravcu, Katedra za farmakologiju i toksikologiju takođe sarađuje i sa klinikama i institutima Veterinarskog fakulteta u Ljubljani

Does thymol under in vitro conditions act as a suppressor of small intestine contractions caused by acetylcholine? Preliminary study

Thymol (5-methyl-2-isopropylphenol) is a phenol derivate which is found in essential oil of thyme (Thymus spp.) and some other plants. Beside several other effects spasmolytic activity of thymol was described. Therefore, the aim of our preliminary study was to determine the impact of thymol on the intensity of small intestine contractions caused by acetylcholine under in vitro conditions. In this study ileums of two Wistar rats were used and the intensity of ileum contraction was measured with the system for work on isolated organs. Different amounts of acetylcholine and thymol were added into the chamber with isolated ileum to exhibit ileum contractions. Results show that thymol suppresses intensity of ileum contractions caused by acetylcholine. In order to determine the exact mechanism of thymol action and to estimate the possible use of thymol as a drug with spasmolytic activity further studies should be performed

Detection of trace amounts of abamectin used as an antiparasitic agent in fallow deer tissues

A sensitive and reliable method for the determination of trace amounts of abamectin in muscles, kidneys and fat tissue of fallow deer is presented. Abamectin was extracted from the tissues with acetonitrile and the extract was cleaned up on a C8 solid-phase extraction cartridge. Abamectin residue was derivatised with trifluoroacetic acid anhydride and 1-methylimidazole, and determined using reversed- phase high-performance liquid chromatography under isocratic conditions and fluorescence detection. The recoveries of the method were high and consistent, ranging from 78% to 90%. The limit of detection of the method was below 1 μg/kg when analysing muscle, kidney and fat tissue. Matrix-matched calibration was used in order to obtain accurate values and to avoid matrix interference