Genetic analysis of MA4079, an aldehyde dehydrogenase homolog, in Methanosarcina acetivorans

When Methanosarcina acetivorans grows on carbon monoxide (CO), it synthesizes high levels of a protein, MA4079, homologous to aldehyde dehydrogenases. To investigate the role of MA4079 in M. acetivorans, mutants lacking the encoding gene were generated and phenotypically analyzed. Loss of MA4079 had no eVect on methylotrophic growth but led to complete abrogation of methylotrophic growth in the presence of even small amounts of CO, which indicated the mutant’s inability to acclimate to the presence of this toxic gas. Prolonged incubation with CO allowed the isolation of a strain in which the eVect of MA4079 deletion is suppressed. The strain, designated Mu3, tolerated the presence of high CO partial pressures even better than the wild type. Immunological analysis using antisera against MA4079 suggested that it is not abundant in M. acetivorans. Comparison of proteins diVerentially abundant in Mu3 and the wild type revealed an elevated level of methyl-coenzyme M reductase and a decreased level of one isoform of carbon monoxide dehydrogenase/acetyl-coenzyme A synthase, which suggests that pleiotropic mutation(s) compensating for the loss of MA4079 aVected catabolism. The data presented point toward a role of MA4079 to enable M. acetivorans to properly acclimate to CO

Annealing effects on the interface and insulator properties of plasma deposited Al SiOxNyHz Si devices

Annealing Effects on the Interface and Insulator Properties of Plasma Deposited Al SiOxNyHz Si Device

Basic electronic properties of a Si H c Si heterostructure solar cells

Basic Electronic Properties of a Si H c Si Heterostructure Solar Cell

Einfluss von inneren und aeusseren Grenzflaechen auf den Wirkungsgrad von photovoltaischen Zellen auf Silizium-Basis Jahresbericht 1991 und 1. Zwischenbericht

Available from TIB Hannover: F95B191 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman

Einfluss von inneren und aeusseren Grenzflaechen auf den Wirkungsgrad von photovoltaischen Zellen auf Silizium-Basis Abschlussbericht

SIGLEAvailable from TIB Hannover: DtF QN1(25,23) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman