129 research outputs found
BAIKAL experiment: status report
We review the present status of the Baikal Neutrino Project and present the
results obtained with the deep underwater neutrino telescope NT-200.Comment: 4 pages, 3 figures. Presented at TAUP 2001 (7th international
workshop on Topics in Astroparticle and Underground Physics), Sep. 2001,
Laboratori Nazionali del Gran Sasso, Assergi, Ital
Simultaneous measurements of water optical properties by AC9 transmissometer and ASP-15 Inherent Optical Properties meter in Lake Baikal
Measurements of optical properties in media enclosing Cherenkov neutrino
telescopes are important not only at the moment of the selection of an adequate
site, but also for the continuous characterization of the medium as a function
of time. Over the two last decades, the Baikal collaboration has been measuring
the optical properties of the deep water in Lake Baikal (Siberia) where, since
April 1998, the neutrino telescope NT-200 is in operation. Measurements have
been made with custom devices. The NEMO Collaboration, aiming at the
construction of a km3 Cherenkov neutrino detector in the Mediterranean Sea, has
developed an experimental setup for the measurement of oceanographic and
optical properties of deep sea water. This setup is based on a commercial
transmissometer. During a joint campaign of the two collaborations in March and
April 2001, light absorption, scattering and attenuation in water have been
measured. The results are compatible with previous ones reported by the Baikal
Collaboration and show convincing agreement between the two experimental
techniques.Comment: 16 pages, submitted to NIM-
ΠΠ½ΡΠ΅ΡΠ»Π΅ΠΉΠΊΠΈΠ½ IL-1Ξ² ΡΡΠΈΠΌΡΠ»ΠΈΡΡΠ΅Ρ ΡΠ΅Π²ΠΈΡΠ°Π»ΠΈΠ·Π°ΡΠΈΡ Ρ ΡΡΡΠ΅Π²ΠΎΠ³ΠΎ ΠΌΠ°ΡΡΠΈΠΊΡΠ° Π½Π°Π·Π°Π»ΡΠ½ΡΠΌΠΈ Ρ ΠΎΠ½Π΄ΡΠΎΡΠΈΡΠ°ΠΌΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° in vitro
Revitalization of decellularized or devitalized matrix scaffolds in tracheal tissue engineering typically involves seeding the autologous recipient cells or allogeneic cells under long-term cultivation. Objective: to study the capability of human nasal chondrocytes for colonization of devitalized scaffolds based on native human tracheal cartilage, with proinflammatory stimulation (cytokine) by adding Interleukin-1-beta (IL-1Ξ²) to the culture medium. Materials and methods. Scaffolds for tracheal tissue engineering were obtained from native human tracheal cartilage through devitalization and laser etching. The scaffold was revitalized by seeding the human nasal chondrocytes. Histological examination was performed after staining with hematoxylin and safranin-O, with further microscopy using a Nikon Eclipse L200 light microscope. X-ray microtomography was performed on a Phoenix nanotom m apparatus. Electron microscopy was performed on a Nova NanoSEM 230 setup. Results. There was statistically significant increase in the intensity of colonization (p = 0.0008) with nasal chondrocytes and stimulation of their migration activity (p < 0.0001) in the presence of IL-1Ξ² compared with the control groups. Conclusion. Addition of proinflammatory cytokine IL-1Ξ² (1 ΞΌg/ml) to the culture medium enhances volumetric seeding of devitalized cartilage scaffold with human nasal chondrocytes, allowing to create highly revitalized materials for tracheal tissue engineering.Π Π΅Π²ΠΈΡΠ°Π»ΠΈΠ·Π°ΡΠΈΡ Π΄Π΅ΡΠ΅Π»Π»ΡΠ»ΡΡΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΈΠ»ΠΈ Π΄Π΅Π²ΠΈΡΠ°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΌΠ°ΡΡΠΈΠΊΡΠΎΠ² Π΄Π»Ρ ΡΠΊΠ°Π½Π΅Π²ΠΎΠΉ ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠΈΠΈ ΡΡΠ°Ρ
Π΅ΠΈ, ΠΊΠ°ΠΊ ΠΏΡΠ°Π²ΠΈΠ»ΠΎ, ΠΏΡΠ΅Π΄ΠΏΠΎΠ»Π°Π³Π°Π΅Ρ Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΠ΅ ΠΌΠ°ΡΡΠΈΠΊΡΠ°-Π½ΠΎΡΠΈΡΠ΅Π»Ρ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π΄ΠΎΠ½ΠΎΡΡΠΊΠΎΠΉ Ρ
ΡΡΡΠ΅Π²ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ Π°ΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ ΡΠ΅ΡΠΈΠΏΠΈΠ΅Π½ΡΠ° ΠΈΠ»ΠΈ Π°Π»Π»ΠΎΠ³Π΅Π½Π½ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ Π² ΡΡΠ»ΠΎΠ²ΠΈΡΡ
Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΡ. Π¦Π΅Π»Ρ ΡΠ°Π±ΠΎΡΡ β ΠΈΠ·ΡΡΠΈΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΊΠΎΠ»ΠΎΠ½ΠΈΠ·Π°ΡΠΈΠΈ Π΄Π΅Π²ΠΈΡΠ°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΌΠ°ΡΡΠΈΠΊΡΠΎΠ² Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π΅ΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΉ Ρ
ΡΡΡΠ΅Π²ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ ΡΡΠ°Ρ
Π΅ΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° Π½Π°Π·Π°Π»ΡΠ½ΡΠΌΠΈ Ρ
ΠΎΠ½Π΄ΡΠΎΡΠΈΡΠ°ΠΌΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΏΡΠΈ Π΄ΠΎΠ±Π°Π²Π»Π΅Π½ΠΈΠΈ ΠΊ ΠΏΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΡΠ΅Π΄Π΅ ΠΏΡΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΈΡΠΎΠΊΠΈΠ½Π° ΠΠ½ΡΠ΅ΡΠ»Π΅ΠΉΠΊΠΈΠ½-1-Π±Π΅ΡΠ° (IL-1Ξ²). ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠ°ΡΡΠΈΠΊΡΡ-Π½ΠΎΡΠΈΡΠ΅Π»ΠΈ Π΄Π»Ρ ΡΠΊΠ°Π½Π΅Π²ΠΎΠΉ ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠΈΠΈ ΡΡΠ°Ρ
Π΅ΠΈ ΠΏΠΎΠ»ΡΡΠ°Π»ΠΈ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π΅ΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΉ Ρ
ΡΡΡΠ΅Π²ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ ΡΡΠ°Ρ
Π΅ΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Π΄Π΅Π²ΠΈΡΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΈ Π»Π°Π·Π΅ΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°Π²Π»Π΅Π½ΠΈΡ. Π Π΅Π²ΠΈΡΠ°Π»ΠΈΠ·Π°ΡΠΈΡ ΠΌΠ°ΡΡΠΈΠΊΡΠΎΠ² ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΏΡΡΠ΅ΠΌ Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ Π½Π°Π·Π°Π»ΡΠ½ΡΡ
Ρ
ΠΎΠ½Π΄ΡΠΎΡΠΈΡΠΎΠ² ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°. ΠΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΏΠΎΡΠ»Π΅ ΠΎΠΊΡΠ°ΡΠΈΠ²Π°Π½ΠΈΡ Π³Π΅ΠΌΠ°ΡΠΎΠΊΡΠΈΠ»ΠΈΠ½ΠΎΠΌ ΠΈ ΡΠ°ΡΡΠ°Π½ΠΈΠ½ΠΎΠΌ-Π Ρ Π΄Π°Π»ΡΠ½Π΅ΠΉΡΠ΅ΠΉ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠΈΠ΅ΠΉ Π½Π° ΡΠ²Π΅ΡΠΎΠ²ΠΎΠΌ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠ΅ Nikon Eclipse L200. Π Π΅Π½ΡΠ³Π΅Π½ΠΎΠ²ΡΠΊΠ°Ρ ΠΌΠΈΠΊΡΠΎΡΠΎΠΌΠΎΠ³ΡΠ°ΡΠΈΡ Π²ΡΠΏΠΎΠ»Π½ΡΠ»Π°ΡΡ Π½Π° Π°ΠΏΠΏΠ°ΡΠ°ΡΠ΅ Phoenix nanotom m. ΠΠ»Π΅ΠΊΡΡΠΎΠ½Π½Π°Ρ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠΈΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»Π°ΡΡ Π½Π° ΡΡΡΠ°Π½ΠΎΠ²ΠΊΠ΅ Nova NanoSEM 230. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΡΡΠ²Π»Π΅Π½ΠΎ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΠΎΠ΅ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΠΈΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΊΠΎΠ»ΠΎΠ½ΠΈΠ·Π°ΡΠΈΠΈ Π½Π°Π·Π°Π»ΡΠ½ΡΠΌΠΈ Ρ
ΠΎΠ½Π΄ΡΠΎΡΠΈΡΠ°ΠΌΠΈ (p = 0,0008) ΠΈ ΡΡΠΈΠΌΡΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΈΡ
ΠΌΠΈΠ³ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ (p < 0,0001) Π² ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠΈ IL-1Ξ² ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΡΠΌΠΈ Π³ΡΡΠΏΠΏΠ°ΠΌΠΈ. ΠΡΠ²ΠΎΠ΄Ρ. ΠΠΎΠ±Π°Π²Π»Π΅Π½ΠΈΠ΅ ΠΏΡΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΈΡΠΎΠΊΠΈΠ½Π° IL-1Ξ² Π² ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ 1 ΠΌΠΊΠ³/ΠΌΠ» ΠΊ ΠΏΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΡΠ΅Π΄Π΅ ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ ΠΎΠ±ΡΠ΅ΠΌΠ½ΠΎΠΌΡ Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ Π΄Π΅Π²ΠΈΡΠ°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ Ρ
ΡΡΡΠ΅Π²ΠΎΠ³ΠΎ ΠΌΠ°ΡΡΠΈΠΊΡΠ° Π½Π°Π·Π°Π»ΡΠ½ΡΠΌΠΈ Ρ
ΠΎΠ½Π΄ΡΠΎΡΠΈΡΠ°ΠΌΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°, ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡ ΡΠΎΠ·Π΄Π°Π²Π°ΡΡ Π²ΡΡΠΎΠΊΠΎΡΠ΅Π²ΠΈΡΠ°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ Π΄Π»Ρ ΡΠΊΠ°Π½Π΅Π²ΠΎΠΉ ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠΈΠΈ ΡΡΠ°Ρ
Π΅ΠΈ
ΠΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½Π°Ρ ΠΎΡΡΠΎΡΠΎΠΏΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠΈΡ ΡΠΊΠ°Π½Π΅ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠ½ΠΎΠΉ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΈ ΡΡΠ°Ρ Π΅ΠΈ, ΡΠΎΠ·Π΄Π°Π½Π½ΠΎΠΉ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π·Π°ΡΠ΅Π»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΌΠ΅Π·Π΅Π½Ρ ΠΈΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΈ ΡΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ Π΄Π΅Π²ΠΈΡΠ°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΠΌΠ°ΡΡΠΈΠΊΡΠ°
Objective: to study the viability of a tissue-engineered graft (TEG) based on a devitalized tracheal scaffold (DTS) seeded with mesenchymal stromal and epithelial cells in an experiment on rabbits with assessment of cytocompatibility and biocompatibility in vivo. Materials and methods. Syngeneic mesenchymal stromal bone marrow cells (MSBMCs) and syngeneic lung epithelial cells of rabbit were obtained. The morphology and phenotype of the MSBMC culture were confirmed via immunofluorescence staining for CD90 and CD271 markers. Pulmonary epithelial cells obtained by enzymatic treatment of minced rabbit lung tissue were stained with CKPan, CK8/18 and CK14 markers characteristic of epithelial cells. The donor trachea was devitalized in three successive freezethawing cycles. Double-layer cell seeding of DTS was performed under static and dynamic culturing. Orthotopic implantation of TEGs was performed at the site of the anterolateral wall defect in the rabbit that was formed as a result of tracheal resection over four rings. Results were evaluated by computed tomography, histological and immunohistochemical analyzes. Results. A TEG implant, based on DTS, with bilayer colonization by cell cultures of rabbit MSBMC and epithelial cells was obtained. Three months after implantation, TEG engraftment was noted, no tracheal wall stenosis was observed. However, slight narrowing of the lumen in the implantation site was noted. Six months after implantation, viability of TEG was confirmed by histological method. Epithelialization and vascularization of the tracheal wall, absence of signs of purulent inflammation and aseptic necrosis were shown. The small narrowing of the lumen of trachea was found to have been caused by chronic inflammation due to irritation of the mucous membrane with suture material. Conclusion. A new model for assessing the viability of a tissue engineering implant when closing a critical airway defect was created. The developed TEG β based on DTS seeded (bilayer) by lung epithelial cells and BMSCs β was successfully used to replace non-extended tracheal defects in an in vivo experiment. The use of tracheal tissue-engineered graft for orthotopic implantation showed biocompatibility with minimal tissue response.Π¦Π΅Π»Ρ. ΠΠ·ΡΡΠΈΡΡ ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ ΡΠΊΠ°Π½Π΅ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠ½ΠΎΠΉ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΈ (Π’ΠΠ) Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π΄Π΅Π²ΠΈΡΠ°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΡΡΠ°Ρ
Π΅Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΌΠ°ΡΡΠΈΠΊΡΠ° (ΠΠ’Π), Π·Π°ΡΠ΅Π»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΌΠ΅Π·Π΅Π½Ρ
ΠΈΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΡΡΡΠΎΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΈ ΡΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ, Π½Π° ΠΌΠΎΠ΄Π΅Π»ΠΈ ΠΎΡΠ΅Π½ΠΊΠΈ ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΠΈ ΡΠΊΠ°Π½Π΅ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠ½ΠΎΠ³ΠΎ ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠ° ΠΏΡΠΈ Π·Π°ΠΊΡΡΡΠΈΠΈ ΠΊΡΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π΄Π΅ΡΠ΅ΠΊΡΠ° Π΄ΡΡ
Π°ΡΠ΅Π»ΡΠ½ΡΡ
ΠΏΡΡΠ΅ΠΉ Ρ ΠΊΡΠΎΠ»ΠΈΠΊΠΎΠ². ΠΡΠ΅Π½ΠΈΡΡ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π» Π’ΠΠ ΠΊ ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΡΡΠ°Π±ΠΈΠ»ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ²Π΅ΡΠ° ΡΡΠ°Ρ
Π΅ΠΈ Π² ΠΎΠ±Π»Π°ΡΡΠΈ ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠΈΠΈ. ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠΎΠ»ΡΡΠ΅Π½Ρ ΡΠΈΠ½Π³Π΅Π½Π½ΡΠ΅ ΠΌΠ΅Π·Π΅Π½Ρ
ΠΈΠΌΠ°Π»ΡΠ½ΡΠ΅ ΡΡΡΠΎΠΌΠ°Π»ΡΠ½ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ ΠΊΠΎΡΡΠ½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π° (ΠΠ‘Π ΠΠ) ΠΈ ΡΠΈΠ½Π³Π΅Π½Π½ΡΠ΅ ΡΠΏΠΈΡΠ΅Π»ΠΈΠΎΡΠΈΡΡ Π»Π΅Π³ΠΊΠΎΠ³ΠΎ ΠΊΡΠΎΠ»ΠΈΠΊΠ°. ΠΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡ ΠΈ ΡΠ΅Π½ΠΎΡΠΈΠΏ ΠΊΡΠ»ΡΡΡΡΡ ΠΠ‘Π ΠΠ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π°Π»ΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠ½ΡΠΌ ΠΎΠΊΡΠ°ΡΠΈΠ²Π°Π½ΠΈΠ΅ΠΌ Π½Π° ΠΌΠ°ΡΠΊΠ΅ΡΡ CD90 ΠΈ CD271. ΠΠ»Π΅ΡΠΊΠΈ Π»Π΅Π³ΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΏΠΈΡΠ΅Π»ΠΈΡ, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ½Π·ΠΈΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠΈ ΠΈΠ·ΠΌΠ΅Π»ΡΡΠ΅Π½Π½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ Π»Π΅Π³ΠΊΠΎΠ³ΠΎ ΠΊΡΠΎΠ»ΠΈΠΊΠ°, Π±ΡΠ»ΠΈ ΠΎΠΊΡΠ°ΡΠ΅Π½Ρ Π½Π° Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠ½ΡΠ΅ Π΄Π»Ρ ΡΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΌΠ°ΡΠΊΠ΅ΡΡ CKPan, CK8/18 ΠΈ CK14. ΠΠ΅Π²ΠΈΡΠ°Π»ΠΈΠ·Π°ΡΠΈΡ Π΄ΠΎΠ½ΠΎΡΡΠΊΠΎΠΉ ΡΡΠ°Ρ
Π΅ΠΈ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π° ΡΡΠ΅ΠΌΡ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΡΠΌΠΈ ΡΠΈΠΊΠ»Π°ΠΌΠΈ Π·Π°ΠΌΠΎΡΠ°ΠΆΠΈΠ²Π°Π½ΠΈΡβΠΎΡΡΠ°ΠΈΠ²Π°Π½ΠΈΡ. ΠΠ²ΡΡ
ΡΠ»ΠΎΠΉΠ½ΠΎΠ΅ Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΠ΅ ΠΠ’Π ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΎ Π² ΡΡΠ»ΠΎΠ²ΠΈΡΡ
ΡΡΠ°ΡΠΈΡΠ½ΠΎΠ³ΠΎ ΠΈ Π΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΡ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½Π° ΠΎΡΡΠΎΡΠΎΠΏΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠΈΡ Π’ΠΠ Π½Π° ΠΌΠ΅ΡΡΠΎ Π΄Π΅ΡΠ΅ΠΊΡΠ° ΠΏΠ΅ΡΠ΅Π΄Π½Π΅Π±ΠΎΠΊΠΎΠ²ΠΎΠΉ ΡΡΠ΅Π½ΠΊΠΈ ΡΡΠ°Ρ
Π΅ΠΈ ΠΊΡΠΎΠ»ΠΈΠΊΠ°, ΡΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ Π² ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ ΡΠ΅Π·Π΅ΠΊΡΠΈΠΈ ΡΡΠ°Ρ
Π΅ΠΈ Π½Π° ΠΏΡΠΎΡΡΠΆΠ΅Π½ΠΈΠΈ ΡΠ΅ΡΡΡΠ΅Ρ
ΠΊΠΎΠ»Π΅Ρ. ΠΡΠ΅Π½ΠΊΠ° ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² Π²ΡΠΏΠΎΠ»Π½Π΅Π½Π° ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΠΎΠΉ ΡΠΎΠΌΠΎΠ³ΡΠ°ΡΠΈΠΈ, Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·ΠΎΠ². Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠΎΠ»ΡΡΠ΅Π½ ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°Ρ Π’ΠΠ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΠ’Π Ρ Π΄Π²ΡΡ
ΡΠ»ΠΎΠΉΠ½ΡΠΌ Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΠ΅ΠΌ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΠΌΠΈ ΠΊΡΠ»ΡΡΡΡΠ°ΠΌΠΈ ΠΠ‘Π ΠΠ ΠΈ ΡΠΏΠΈΡΠ΅Π»ΠΈΠΎΡΠΈΡΠΎΠ² ΠΊΡΠΎΠ»ΠΈΠΊΠ°. Π§Π΅ΡΠ΅Π· 3 ΠΌΠ΅Ρ. ΠΏΠΎΡΠ»Π΅ ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠΈΠΈ ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΎΡΡ ΠΏΡΠΈΠΆΠΈΠ²Π»Π΅Π½ΠΈΠ΅ Π’ΠΠ, ΡΡΠ΅Π½ΠΎΠ·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΡΠ΅Π½ΠΊΠΈ ΡΡΠ°Ρ
Π΅ΠΈ Π½Π΅ Π½Π°Π±Π»ΡΠ΄Π°Π»ΠΎΡΡ, ΠΎΠ΄Π½Π°ΠΊΠΎ ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΎΡΡ Π½Π΅Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ΅ ΡΡΠΆΠ΅Π½ΠΈΠ΅ ΠΏΡΠΎΡΠ²Π΅ΡΠ° Π² ΠΎΠ±Π»Π°ΡΡΠΈ ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠΈΠΈ. ΠΠ° 6-ΠΉ ΠΌΠ΅Ρ. ΠΏΠΎΡΠ»Π΅ ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠΈΠΈ ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ ΡΠΊΠ°Π½Π΅ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠ½ΠΎΠΉ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΈ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π°Π»Π°ΡΡ Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ. ΠΠΎΠΊΠ°Π·Π°Π½Π° ΡΠΏΠΈΡΠ΅Π»ΠΈΠ·Π°ΡΠΈΡ ΠΈ Π²Π°ΡΠΊΡΠ»ΡΡΠΈΠ·Π°ΡΠΈΡ ΡΡΠ΅Π½ΠΊΠΈ ΡΡΠ°Ρ
Π΅ΠΈ, ΠΎΡΡΡΡΡΡΠ²ΠΈΠ΅ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² Π³Π½ΠΎΠΉΠ½ΠΎΠ³ΠΎ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΡ ΠΈ Π°ΡΠ΅ΠΏΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π½Π΅ΠΊΡΠΎΠ·Π°. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π° ΠΏΡΠΈΡΠΈΠ½Π° Π½Π΅Π±ΠΎΠ»ΡΡΠΎΠ³ΠΎ ΡΡΠΆΠ΅Π½ΠΈΡ ΠΏΡΠΎΡΠ²Π΅ΡΠ° ΡΡΠ°Ρ
Π΅ΠΈ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΠ΅, Π²ΡΠ·Π²Π°Π½Π½ΠΎΠ΅ ΡΠ°Π·Π΄ΡΠ°ΠΆΠ΅Π½ΠΈΠ΅ΠΌ ΡΠ»ΠΈΠ·ΠΈΡΡΠΎΠΉ ΡΠΎΠ²Π½ΡΠΌ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»ΠΎΠΌ. ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠΎΠ»ΡΡΠ΅Π½Π° ΠΌΠΎΠ΄Π΅Π»Ρ Π΄Π»Ρ ΠΎΡΠ΅Π½ΠΊΠΈ ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΠΈ ΡΠΊΠ°Π½Π΅ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠ½ΠΎΠ³ΠΎ ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠ° ΠΏΡΠΈ Π·Π°ΠΊΡΡΡΠΈΠΈ ΠΊΡΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π΄Π΅ΡΠ΅ΠΊΡΠ° Π΄ΡΡ
Π°ΡΠ΅Π»ΡΠ½ΡΡ
ΠΏΡΡΠ΅ΠΉ. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π½Π°Ρ Π’ΠΠ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΠ’Π, Π΄Π²ΡΡ
ΡΠ»ΠΎΠΉΠ½ΠΎ Π·Π°ΡΠ΅Π»Π΅Π½Π½ΠΎΠ³ΠΎ ΡΠΏΠΈΡΠ΅Π»ΠΈΠΎΡΠΈΡΠ°ΠΌΠΈ Π»Π΅Π³ΠΊΠΎΠ³ΠΎ ΠΈ ΠΠ‘Π ΠΠ, Π±ΡΠ»Π° ΡΡΠΏΠ΅ΡΠ½ΠΎ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½Π° Π΄Π»Ρ Π·Π°ΠΌΠ΅ΡΠ΅Π½ΠΈΡ Π½Π΅ΠΏΡΠΎΡΡΠΆΠ΅Π½Π½ΡΡ
Π΄Π΅ΡΠ΅ΠΊΡΠΎΠ² ΡΡΠ°Ρ
Π΅ΠΈ Π² ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ΅ in vivo. ΠΠΈΠ½ΠΈΠΌΠ°Π»ΡΠ½Π°Ρ ΡΠΊΠ°Π½Π΅Π²Π°Ρ ΡΠ΅Π°ΠΊΡΠΈΡ Π½Π° Π’ΠΠ ΡΡΠ°Ρ
Π΅ΠΈ Π±ΡΠ»Π° ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½Π° Π±ΠΈΠΎΡΠΎΠ²ΠΌΠ΅ΡΡΠΈΠΌΠΎΡΡΡΡ ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠ°
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