Relationship between the prevalence of <i>A.</i><i>astaci</i> (in %; estimated as the proportion of individuals testing positive) in analysed French signal crayfish populations and the average pathogen load (expressed as log-transformed PFU-values) detected in infected individuals (with agent level A2 or higher) from each population.

<p>The equation characterising the model estimated by logistic regression (dashed line) is given in the upper right corner; removal of two outlier populations with highest average pathogen load (indicated by empty circles) did not change the model substantially (dotted line, equation not shown). Note that when a quasibinomial instead of binomial distribution of errors is used (due to overdispersion in data), the increasing trend is non-significant.</p

Map of France with administrative division to regions (dark-bordered areas) and departments within them (light-bordered areas), showing the distribution of the invasive signal crayfish <i>Pacifastacus leniusculus</i> (small empty circles; based on [4], [19], [21], [44]) and approximate location of analysed populations (triangles), and the recent status of the native white-clawed crayfish <i>Austropotamobius pallipes</i> (green shading) and reported cases of its mass mortalities (red crosses and black dots).

<p>Distribution <i>of A. pallipes</i> is based on a 2006 survey <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070157#pone.0070157-Collas2" target="_blank">[23]</a> (white: no known population in a department, pale green: 1–5, medium: 6–25, dark: 25–150 populations). Red crosses with years indicate mass mortalities most likely caused by crayfish plague reported since the 1990s (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070157#pone.0070157-Machino1" target="_blank">[10]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070157#pone.0070157-Collas1" target="_blank">[12]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070157#pone.0070157-Neveu1" target="_blank">[13]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070157#pone.0070157-Neveu2" target="_blank">[14]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070157#pone.0070157-Neveu3" target="_blank">[15]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070157#pone.0070157-Papin1" target="_blank">[45]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070157#pone.0070157-Neveu5" target="_blank">[46]</a>; T. Duperray and T. Pantarotto, pers. comm.); additional mortalities ascribed to crayfish plague were reported between 2001 and 2005 from departments marked by black dots (according to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070157#pone.0070157-Collas2" target="_blank">[23]</a>). Bold crosses mark outbreaks in which <i>A. astaci</i> has been confirmed by molecular detection and genotyped (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070157#s4" target="_blank">Discussion</a>). The prevalence of <i>Aphanomyces astaci</i> in sampled signal crayfish populations is expressed by colour: no reliable detection of the pathogen (white triangles), low prevalence (1–30%, yellow), medium prevalence (31–60%, orange) and high prevalence (61–100%, red triangles); the number of analysed individuals in the respective population is indicated by symbol size (small triangle: <10 individuals, large triangle: 10+ individuals). Regions discussed in the text are abbreviated: Al – Alsace, Au – Auvergne, Ba-No – Basse-Normandie, Ce – Centre, Ch-Ar – Champagne-Ardenne, La-Ro – Languedoc-Rousillon, Li – Limousin, Lo – Lorraine, Po-Ch – Poitou-Charentes, Rh-Al – Rhône-Alpes; Lake Geneva (Lac Léman) is marked by L within the triangle. The distance scale depends on latitude (top: 51.5°N, bottom: 41°N) and reflects the map projection.</p

Experiment 1 (glochidia infections)

Raw data from Experiment 1 (glochidia infections), see Readme file for detail

Experiment 3b (impacts on Baltic fish)

experimental data on population impacts of A. woodiana invasion for Baltic populations of R. amaru

Experiment 3a (impact on Danubian fish)

experimental data on population impacts of A. woodiana invasion for Danubian populations of R. amaru