Comparative molecular analysis of old olive (Olea europaea L.) genotypes from Eastern Mediterranean Region of Turkey

Olive is an important Mediterranean tree species having many different ways of utilizations. The olive grove is an important farming sector in Turkey and dates back to thousands years, particularly in Anatolia. An historical culture of olive resulted in a broad genetic base for olive which is a long-lived tree. We compared the genetic profiles of six old olive cultivars from an Eastern Mediterranean Region of Turkey to 15 modern Turkish olive cultivars from different geographical origins. The RAPD profiles successfully clarified the molecular relationships among the genotypes tested. Seventeen RAPD primers generated 153 reproducible bands, 81% of which were polymorphic. The data were subjected tocluster and principle coordinates analyses. Cluster analysis supported three small groups based on geographical origins and these groups did not include any of the old cultivars. The old cultivars scattered around the ungrouped accessions and formed four subgroups. Principle coordinate resulted in similar overall patterns. Our results revealed that although sampled from a relatively narrow region, the old olive cultivars have broad genetic basis and are closely related to some of present-daycultivars/clones

Molecular and pomological diversity among pomegranate (Punica granatum L.) cultivars in Eastern Mediterranean region of Turkey

Pomegranate (Punica granatum L.) is an important fruit species for Turkey where many cultivars are being cultivated. In this study, we determined the fruit characteristics and RAPD band patterns of sixlocal cultivars from Hatay, Turkey. Our results demonstrated that there is a great level of morphological variation. The principle component analysis of 18 quantitative fruit characteristics revealed that fruitweight, aril number/fruit, peel color and soluble solids/acidity ratio are important traits for discriminating the cultivars tested. The UPGMA cluster of fruit characteristics indicated that ‘Katýrbaý’and ‘Kan narý’ were similar to each other and they were separated from rest of the cultivars. Twenty-two RAPD primers generated total of 106 reproducible bands 22% of which were polymorphic. The UPGMAdendrogram of RAPD data showed that ‘Tatlý nar’ and ‘erife’ were very closely related while ‘ncekabuk’ is distinct from the other cultivars. As a result, discrepancies were detected between morphological and molecular data. Therefore, we confirmed that diversity among the fruitcharacteristics were not good indication of genetic relatedness while molecular tools are valuable to study such similarities

Molecular and pomological diversity among pomegranate (Punica granatum L.) cultivars in Eastern Mediterranean region of Turkey

Pomegranate (Punica granatum L.) is an important fruit species for Turkey where many cultivars are being cultivated. In this study, we determined the fruit characteristics and RAPD band patterns of six local cultivars from Hatay, Turkey. Our results demonstrated that there is a great level of morphological variation. The principle component analysis of 18 quantitative fruit characteristics revealed that fruit weight, aril number/fruit, peel color and soluble solids/acidity ratio are important traits for discriminating the cultivars tested. The UPGMA cluster of fruit characteristics indicated that 'Katirbaşi' and 'Kan nari' were similar to each other and they were separated from rest of the cultivars. Twenty-two RAPD primers generated total of 106 reproducible bands 22% of which were polymorphic. The UPGMA dendrogram of RAPD data showed that 'Tatli nar' and 'Şerife' were very closely related while 'İncekabuk' is distinct from the other cultivars. As a result, discrepancies were detected between morphological and molecular data. Therefore, we confirmed that diversity among the fruit characteristics were not good indication of genetic relatedness while molecular tools are valuable to study such similarities. © 2008 Academic Journals

Karyological features of wild and cultivated forms of myrtle (Myrtus communis, Myrtaceae)

ABSTRACT. Myrtle is an evergreen shrub or small tree widespread throughout the Mediterranean region. In Turkey, both cultivated and wild forms, differing in plant and fruit size and fruit composition, can be found. These differences may have resulted from the domestication of the cultivated form over a long period of time. We investigated whether wild and cultivated forms of myrtle differ in karyological features (i.e., number of somatic chromosomes and relative genome size). We sampled two wild forms and six cultivated types of myrtle. All the samples had the same chromosome number (2n = 2x = 22). The results were confirmed by 4’,6-diamidino-2-phenylindole (DAPI) flow cytometry. Only negligible variation (~3%) in relative fluorescence intensity was observed among the different myrtle accessions, with wild genotypes having the smallest values. We concluded that despite considerable morphological differentiation, cultivated and wild myrtle genotypes in Turkey have similar karyological features