Development of miniature hydrophone with PZT flim deposited by hydrothermal method

Abstract -A needle type miniature hydrophone was developed by hydrothermally depositing PZT poly-crystal film with thickness of about 10µm on an end of a titanium wire with diameter of 0.3mm in this study. It is desirable that hydrophone should have as small shape as possible in order to avoid from disturbance in acoustic field by itself. Since the body of hydrophone developed in this study is a titanium wire with diameter of 0.3mm, disturbance in acoustic field measurement by our hydrophone can be reduced remarkably. It was confirmed that receiving sensitivity of our hydrophone was about -280 dB (0dB=1V/µPa) in frequency range from 1 MHz to 10 MHz

High glucose induces platelet derived growth factor-C via carbohydrate response element binding protein in glomeruｌar mesangial cells

Persistent high concentration of glucose causes cellular stress and damage in diabetes via derangement of gene expressions. We previously reported high glucose activates hypoxia-inducible factor-1αand downstream gene expression in mesangial cells, leading to an extracellular matrix expansion in the glomeruli. A glucose-responsive transcription factor carbohydrate response element-binding protein (ChREBP) is a key mediator for such perturbation of gene regulation. To provide insight into glucose-mediated gene regulation in mesangial cells, we performed chromatin immunoprecipitation followed byDNAmicroarray analysis and identified platelet-derived growth factor-C (PDGF-C) as a novel target gene of ChREBP In streptozotocin-induced diabetic mice, glomerular cells showed a significant increase inPDGF-C expression; the ratio ofPDGF-C-positive cells to the total number glomerular cells demonstrated more than threefold increase when compared with control animals. In cultured human mesangial cells, high glucose enhanced expression ofPDGF-C protein by 1.9-fold. Knock-down of ChREBPabrogated this induction response. UpregulatedPDGF-C contributed to the production of typeIVand typeVIcollagen, possibly via an autocrine mechanism. Interestingly, urinaryPDGF-C levels in diabetic model mice were significantly elevated in a fashion similar to urinary albumin. Taken together, we hypothesize that a high glucose-mediated induction ofPDGF-C via ChREBPin mesangial cells contributes to the development of glomerular mesangial expansion in diabetes, which may provide a platform for novel predictive and therapeutic strategies for diabetic nephropathy.博士（医学）旭川医科大

A Novel In Vitro Simulator to Investigate Promotion of Reconstruction of Damaged Neuronal Cell Colony Differentiated from iPS Cells with the Aid of Micro Dynamic Stimulation

Neuronal cells are equipped with the function of a sensor that senses stimulation and elongates neurites to connect nearby neuronal cells in forming a neuronal network, as they are generally said to be hard to recover from physical damage, such as in the case of a spinal cord injury. Therefore, in this study, a novel in vitro simulator in which micro dynamic stimulations are applied to a damaged neuronal cell colony artificially is proposed to investigate the possibility of promoting the reconstruction of damaged neuronal cells on a colony basis. A neuronal cell colony differentiated from iPS cells is physically damaged by cutting off treatment, and micro dynamic stimulations are applied to the colony by utilizing a developed mini-vibration table system. NeuroFluor NeuO is used to establish a method for fluorescent staining of the living neuronal cells, and morphologies of the reconstructing neurons are analysed, revealing a relationship between the stimulation and the reconstructing process of the damaged neurons. It is found that significant differences are observed in the reconstructing efficiency between the statically cultured damaged neuronal cell colony and the dynamically stimulated one. The results suggest that applying appropriate micro dynamic stimulations is a promising approach to promote the reconstruction of a damaged neuronal cell colony

Pressure and Flow Rate Performance of Piezoelectric Fans

A piezoelectric fan is a flexible cantilever beam whose vibration is actuated by means of a piezoelectric material. Such fans have been employed for the enhancement of heat transfer by increasing the fluid circulation in regions which are otherwise stagnant. The main focus of past studies has been to predict and describe the heat transfer achievable using these devices, as well as the flow field generated by vibrating cantilevers. In order to directly compare these fans with their traditional counterparts such as small axial fans, the present work casts the performance of piezofans in terms of a characteristic often used to represent conventional fans, namely the fan curve. The primary focus of this paper is to determine the relationship between the pressure and the flow rate generated by miniature piezoelectric fans. Experimental measurements are obtained for fans with operating frequencies of 60 and 113 Hz. The maximum flow rate conditions yield nearly 30 l/min, while the greatest static pressure generated is found to be 6 Pa. The performance is highly dependent on both the vibration amplitude and frequency. Predictive relationships are developed to describe the experimental trends and provide insight into the sensitivity of pressure and flow rate to these operating parameters. These fans are directly compared to two commercially available axial fans, both in terms of overall performance and efficiency with which energy is imparted to the fluid. Piezoelectric fans are found to compare quite favorably using either of these performance metrics with a nearly order-of-magnitude increase in fan efficiency. A secondary focus of this paper is to explore the effects of fan installation details on fan performance. The proximity of surrounding walls is considered through the use of three different enclosures within which the fan is mounted. Effective inlet areas from which the air enters the fan are also identified. This paper provides a practical framework for determining the optimal placement and configuration for these fans in prototypical applications