Volume CVI, Number 10, January 13, 1989

WOS: 000188054500005Two subspecies of mistletoe (Viscum album L. ssp. album and ssp. abietis) growing on lime and pine trees, respectively, were investigated for the monosaccharides and polyols by GC-MS spectrometry. Arabinose, xylose, glucose, galactose, mannose, xylitol and inositol were determined in methanol extracts following the acidic hydrolysis. Sugar contents of the leaves were expressed as percentage on dry weight. Xylose content was the same (1.5%) in each species, whereas the other saccharides varied. V. album ssp. abietis (collected from pine trees) were found containing significantly higher percentage of glucose (29.0%) and galactose (44.0%) than V. album ssp. album (collected from lime trees) (9.0% and 17.0%, respectively). In contrast, mannose, arabinose and sugar alcohol percentages were higher in ssp. album. Mannose content was 3.5% for ssp. album whereas 1.0% for ssp. abietis. 3.0% arabinose were determined in the former and 2.0% in the latter. Xylitol and inositol percentages were 8.0% and 58.0% for ssp. album and 1.5% and 21.0% for ssp. abietis, respectively. These results indicate that saccharide composition of mistletoes depends upon the subspecies of the plant and the host tree

An Acidic Triterpene Glycoside From Cephalaria-Transsylvanica

WOS: A1994PH22500043PubMed ID: 7765438Transsylvanoside B, which is a new acidic triterpene glycoside, was isolated from dried flowers of Cephalaria transsylvanica. The structure was determined as 3-O-[beta-D-glucopyranosyl(1 --> 2)-beta-D-xylopyranosyl(1 --> 4)-alpha-L-rhamnopyranosyl (1 --> 4)-beta-D-xylopyranosyl]-3 beta,23-dihydroxy Delta(12)-oleanen-28-carboxylic acid

New triterpenic saponins from Cephalaria transsylvanica

WOS: 000179432700016Three new triterpenic saponins, 3-O-[beta-D-xylopyranosyl (1-->4)-beta-D-glucopyranosyl (1-->4)-beta-D-glucopyranosyl (1-->3)-alpha-L-rhamnopyranosyl (1-->4)-beta-D-xylopyranosyl] hederagenin 28-O-[beta-D-glucopyranosyl (1-->4)-beta-D-glucopyranosyl] ester (1), 3-O-[beta-D-glucopyranosyl (1-->4)-beta-D-glucopyranosyl (1-->3)-alpha-L-rhamnopyranosyl (1-->4)-beta-D-xylopyranosyll hederagenin 28-O-[beta-D-glucopyranosyll ester 2) and 3-O-[beta-D-glucopyranosyl (1-->3)- alpha-L-rhamnopyranosyl (1-->4)-beta-D-xylopyranosyl] hederagenin(3) were isolated from Cephalaria transsylvanica. After cleavage of the ester-glycosidic linkage of the bisdesmosidic compounds, two new prosapogenins, 3-O-[beta-D-xylopyranosyl (1-->4)-beta-D-glucpyranosyl (1-->4)-beta-D-glucopyranosyl (1-->3)- alpha-L-rhamnopyranosyl (1-->4)-beta-D-xylopyranosyl] hederagenin (4) and 3-O-[beta-D-glucopyranosyl (1-->4)- beta-D-glucopyranosyl (1-->3)- alpha-L-rhamnopyranosyl (1-->4)- beta-D-xylopyranosyl] hederagenin (5) were also isolated. The structures of the compounds were established by chemical and spectroscopic means

Rapid profiling and identification of triterpene saponins in three different Cephalaria species by UPLC-ESI-MS/MS

Triterpene saponins are bioactive glycosides which are responsible for the defense mechanism of the plants and have extensive structural diversity in many plant species. In this study, profile of triterpene saponins was determined in the crude n-BuOH extracts of Cephalaria hirsuta, Cephalaria elazigensis var. elazigensis, and Cephalaria procera taxa (Caprifoliaceae) by ultra pressure liquid chromatography coupled with electrospray ionization tandem mass spectrometry. The UPLC-ESI-MS/MS method has been validated to provide linearity, recovery, precision (repeatability), limit of detection and limit of quantification parameters for quantitative analysis. Identification and quantification of the compounds were based on a group of fragment ions and ESI-MS spectra; precursor ions were observed as [M+Na]+ and [M+H]+ in positive mode for molecular mass information. Thirty nine saponins were identified and quantified by reversed-phase liquid chromatography (RP-LC) and they were separated on C18 reverse phase column (50 mm x 2.1 mm, 1.7 µm) by methanol-water gradient containing 0.1 mM ammonium formate solution as a mobile phase. Among the detected compounds, 28 compounds in C. hirsuta, 4 compounds in C. elazigensis var. elazigensis and 22 compounds in C. procera were identified and quantified based on their retention times and mass spectra in comparison with the data of references which were isolated in our previous studies. In this study, rapid application of an UPLC-ESI-MS/MS approach for resolving the chemical content of complex crude n-BuOH extracts of three Cephalaria species were presented, for the first time. © 2018 ACG Publications. All rights reserved.University of Delaware Research Foundation: 2015/FEN/061The authors gratefully thank Ege University, Center for Drug Research and Development and Pharmacokinetic Applications (ARGEFAR) for providing Waters TQD UPLC-ESI-MS/MS system and Ege University Research Foundation (2015/FEN/061) for financial support. -

Monosaccharides and polyols from mistletoes (Viscum album L.) growing on two different host species

Two subspecies of mistletoe (Viscum album L. ssp. album and ssp. abietis) growing on lime and pine trees, respectively, were investigated for the monosaccharides and polyols by GC-MS spectrometry. Arabinose, xylose, glucose, galactose, mannose, xylitol and inositol were determined in methanol extracts following the acidic hydrolysis. Sugar contents of the leaves were expressed as percentage on dry weight. Xylose content was the same (1.5%) in each species, whereas the other saccharides varied. V. album ssp. abietis (collected from pine trees) were found containing significantly higher percentage of glucose (29.0%) and galactose (44.0%) than V. album ssp. album (collected from lime trees) (9.0% and 17.0%, respectively). In contrast, mannose, arabinose and sugar alcohol percentages were higher in ssp. album. Mannose content was 3.5% for ssp. album whereas 1.0% for ssp. abietis. 3.0% arabinose were determined in the former and 2.0% in the latter. Xylitol and inositol percentages were 8.0% and 58.0% for ssp. album and 1.5% and 21.0% for ssp. abietis, respectively. These results indicate that saccharide composition of mistletoes depends upon the subspecies of the plant and the host tree