Effect of farnesol on Candida dubliniensis morphogenesis

Cell–cell signalling in Candida albicans is a known phenomenon and farnesol was identified as a quorum sensing molecule determining the yeast morphology. The aim of this work was to verify if farnesol had a similar effect on Candida dubliniensis, highlighting the effect of farnesol on Candida spp. morphogenesis. Methods and Results: Two different strains of C. dubliniensis and one of C. albicans were grown both in RPMI 1640 and in serum in the presence of absence of farnesol. At 150 μmol l -1 farnesol the growth rate of both Candida species was not affected. On the contrary, farnesol inhibited hyphae and pseudohyphae formation in C. dubliniensis. Conclusion: Farnesol seems to mediate cell morphology in both Candida species. Significance and Impact of the Study: The effect of farnesol on C. dubliniensis morphology was not reported previously.Fundação para a Ciência e a Tecnologia (FCT

Response to Comment on “Pushing the frontiers of density functionals by solving the fractional electron problem”

Gerasimov et al. claim that the ability of DM21 to respect fractional charge (FC) and fractional spin (FS) conditions outside of the training set has not been demonstrated in our paper. This is based on (i) asserting that the training set has a ~50% overlap with our bond-breaking benchmark (BBB) and (ii) questioning the validity and accuracy of our other generalization examples. We disagree with their analysis and believe that the points raised are either incorrect or not relevant to the main conclusions of the paper and to the assessment of general quality of DM2

Pushing the frontiers of density functionals by solving the fractional electron problem

Density functional theory describes matter at the quantum level, but all popular approximations suffer from systematic errors that arise from the violation of mathematical properties of the exact functional. We overcame this fundamental limitation by training a neural network on molecular data and on fictitious systems with fractional charge and spin. The resulting functional, DM21 (DeepMind 21), correctly describes typical examples of artificial charge delocalization and strong correlation and performs better than traditional functionals on thorough benchmarks for main-group atoms and molecules. DM21 accurately models complex systems such as hydrogen chains, charged DNA base pairs, and diradical transition states. More crucially for the field, because our methodology relies on data and constraints, which are continually improving, it represents a viable pathway toward the exact universal functiona

Antimicrobial activity of the essential oil of Cymbopogon citratus (DC) Stapf. on Staphylococcus spp., Streptococcus mutans and Candida spp.

Medicinal plants with fungicide action, antibacterial and anti-inflammatory effects are under investigation. The main purpose of this work was to evaluate the antimicrobial activity of the essential oil from Cymbopogon citratus (DC) Stapf. on strains of Staphylococcus spp., Streptococcus mutans and Candida spp. with planktonic and biofilm growth. To study the micro-organisms in planktonic cells, the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined by using 9 clinical strains for each species and 1 ATCC (American Type Culture Collection) from C. albicans, C. tropicalis, C. glabrata, S. aureus, S. epidermidis and S. mutans. In order to evaluate the effects of the essential oils on biofilms, strains of S. aureus (ATCC 6538), S. mutans (ATCC 35688) and C. albicans (ATCC 18804) were used. The biofilm was formed on acrylic resin discs with isolated micro-organisms or in associations. The number of colony-forming-units (CFU) obtained in each biofilm (CFU/ml) was submitted to Student's t statistical test. The results demonstrated that the essential oil of Cymbopogon citratus showed microbiostatic and microbicidal activity against all tested strains. The average CFU/ml for the biofilm of S. aureus, S. mutans and C. albicans, whether isolated or in association, was lower in the group treated with essential oil than in the control group

Making Growth More Environmentally Sustainable in Germany

This document analyses German environmental policies, including, among others, discussion of air and water quality policy, use of the waste management hierarchy approach and of voluntary agreements. The German public has been highly sensitive to environmental concerns, leading to many policy initiatives in this area since the early 1980s. Significant environmental improvements have been achieved, using an approach frequently based on detailed regulations, often developed with the close involvement of industry and even individual polluters. There has developed a tendency to relieve some of the heaviest polluters from the most stringent regulation, on the grounds of competitiveness concerns, and some polluting activities receive considerable direct or indirect subsidies; these policies will increasingly make it unnecessarily expensive to meet environmental targets. Objectives need to be defined more clearly and consistently and a less discriminatory approach to taxation and ... Ce document analyse les politiques environnementales de l’Allemagne, en particulier les politiques pour l'air et l'eau, l'utilisation de l'approche de la hiérarchie de traitement des déchets et des approches volontaires. L'opinion allemande est très sensibilisée aux problèmes d'environnement, ce qui a mené depuis le début des années 80 à de nombreuses initiatives de politique dans ce domaine. Des progrès significatifs ont été accomplis, en utilisant le plus souvent une approche fondée sur des réglementations détaillées, développées en étroite coopération avec l'industrie et même les pollueurs individuels. En général, les pollueurs les plus importants ont eu tendance à être exemptés des réglementations les plus strictes, du fait de préoccupations de compétitivité, et certaines activités polluantes reçoivent des subventions directes ou indirectes considérables; ces politiques rendront de plus en plus inutilement coûteuse la réalisation des objectifs environnementaux. Les objectifs ...sustainable development, environmental policy, Germany, Allemagne, politique environnementale, développement durable