302 research outputs found
Data-Driven Computing in Dynamics
We formulate extensions to Data Driven Computing for both distance minimizing
and entropy maximizing schemes to incorporate time integration. Previous works
focused on formulating both types of solvers in the presence of static
equilibrium constraints. Here formulations assign data points a variable
relevance depending on distance to the solution and on maximum-entropy
weighting, with distance minimizing schemes discussed as a special case. The
resulting schemes consist of the minimization of a suitably-defined free energy
over phase space subject to compatibility and a time-discretized momentum
conservation constraint. The present selected numerical tests that establish
the convergence properties of both types of Data Driven solvers and solutions.Comment: arXiv admin note: substantial text overlap with arXiv:1702.0157
Data Driven Computing with Noisy Material Data Sets
We formulate a Data Driven Computing paradigm, termed max-ent Data Driven Computing, that generalizes distance-minimizing Data Driven Computing and is robust with respect to outliers. Robustness is achieved by means of clustering analysis. Specifically, we assign data points a variable relevance depending on distance to the solution and on maximum-entropy estimation. The resulting scheme consists of the minimization of a suitably-defined free energy over phase space subject to compatibility and equilibrium constraints. Distance-minimizing Data Driven schemes are recovered in the limit of zero temperature. We present selected numerical tests that establish the convergence properties of the max-ent Data Driven solvers and solutions
Data-driven computational mechanics
We develop a new computing paradigm, which we refer to as data-driven computing, according to which calculations are carried out directly from experimental material data and pertinent constraints and conservation laws, such as compatibility and equilibrium, thus bypassing the empirical material modeling step of conventional computing altogether. Data-driven solvers seek to assign to each material point the state from a prespecified data set that is closest to satisfying the conservation laws. Equivalently, data-driven solvers aim to find the state satisfying the conservation laws that is closest to the data set. The resulting data-driven problem thus consists of the minimization of a distance function to the data set in phase space subject to constraints introduced by the conservation laws. We motivate the data-driven paradigm and investigate the performance of data-driven solvers by means of two examples of application, namely, the static equilibrium of nonlinear three-dimensional trusses and linear elasticity. In these tests, the data-driven solvers exhibit good convergence properties both with respect to the number of data points and with regard to local data assignment. The variational structure of the data-driven problem also renders it amenable to analysis. We show that, as the data set approximates increasingly closely a classical material law in phase space, the data-driven solutions converge to the classical solution. We also illustrate the robustness of data-driven solvers with respect to spatial discretization. In particular, we show that the data-driven solutions of finite-element discretizations of linear elasticity converge jointly with respect to mesh size and approximation by the data set
Data Driven Computing
Data Driven Computing is a new field of computational analysis which uses provided data to directly produce predictive outcomes. This thesis first establishes definitions of Data-Driven solvers and working examples of static mechanics problems to demonstrate efficacy. Significant extensions are then explored to both accommodate noisy data sets and apply the deveoloped methods to dynamic problems within mechanics. Possible method improvements discuss incorporation of data quality metrics and adaptive data sampling, while new applications focus on multi-scale analysis and the need for public databases to support constitutive data collaboration
Model-Free Data-Driven Inelasticity
We extend the Data-Driven formulation of problems in elasticity of
Kirchdoerfer and Ortiz (2016) to inelasticity. This extension differs
fundamentally from Data-Driven problems in elasticity in that the material data
set evolves in time as a consequence of the history dependence of the material.
We investigate three representational paradigms for the evolving material data
sets: i) materials with memory, i.e., conditioning the material data set to the
past history of deformation; ii) differential materials, i.e., conditioning the
material data set to short histories of stress and strain; and iii) history
variables, i.e., conditioning the material data set to ad hoc variables
encoding partial information about the history of stress and strain. We also
consider combinations of the three paradigms thereof and investigate their
ability to represent the evolving data sets of different classes of inelastic
materials, including viscoelasticity, viscoplasticity and plasticity. We
present selected numerical examples that demonstrate the range and scope of
Data-Driven inelasticity and the numerical performance of implementations
thereof.Comment: Minor revisions: affiliations, acknowledgment
Model-Free Data-Driven inelasticity
We extend the Data-Driven formulation of problems in elasticity of Kirchdoerfer and Ortiz (2016) to inelasticity. This extension differs fundamentally from Data-Driven problems in elasticity in that the material data set evolves in time as a consequence of the history dependence of the material. We investigate three representational paradigms for the evolving material data sets: (i) materials with memory, i. e., conditioning the material data set to the past history of deformation; (ii) differential materials, i. e., conditioning the material data set to short histories of stress and strain; and (iii) history variables, i. e., conditioning the material data set to ad hoc variables encoding partial information about the history of stress and strain. We also consider combinations of the three paradigms thereof and investigate their ability to represent the evolving data sets of different classes of inelastic materials, including viscoelasticity, viscoplasticity and plasticity. We present selected numerical examples that demonstrate the range and scope of Data-Driven inelasticity and the numerical performance of implementations thereof
Reporter Assays for Ebola Virus Nucleoprotein Oligomerization, Virion-Like Particle Budding, and Minigenome Activity Reveal the Importance of Nucleoprotein Amino Acid Position 111
For highly pathogenic viruses, reporter assays that can be rapidly performed are critically needed to identify potentially functional mutations for further study under maximal containment (e.g., biosafety level 4 [BSL-4]). The Ebola virus nucleoprotein (NP) plays multiple essential roles during the viral life cycle, yet few tools exist to study the protein under BSL-2 or equivalent containment. Therefore, we adapted reporter assays to measure NP oligomerization and virion-like particle (VLP) production in live cells and further measured transcription and replication using established minigenome assays. As a proof-of-concept, we examined the NP-R111C substitution, which emerged during the 20132016 Western African Ebola virus disease epidemic and rose to high frequency. NP-R111C slightly increased NP oligomerization and VLP budding but slightly decreased transcription and replication. By contrast, a synthetic charge-reversal mutant, NP-R111E, greatly increased oligomerization but abrogated transcription and replication. These results are intriguing in light of recent structures of NP oligomers, which reveal that the neighboring residue, K110, forms a salt bridge with E349 on adjacent NP molecules. By developing and utilizing multiple reporter assays, we find that the NP-111 position mediates a complex interplay between NP\u27s roles in protein structure, virion budding, and transcription and replication
Globular-shaped variable lymphocyte receptors B antibody multimerized by a hydrophobic clustering in hagfish
In hagfish and lampreys, two representative jawless vertebrates, the humoral immunity is directly mediated by variable lymphocyte receptors B (VLRBs). Both monomeric VLRBs are structurally and functionally similar, but their C-terminal tails differ: lamprey VLRB has a Cys-rich tail that forms disulfide-linked pentamers of dimers, contributing to its multivalency, whereas hagfish VLRB has a superhydrophobic tail of unknown structure. Here, we reveal that VLRBs obtained from hagfish plasma have a globular-shaped multimerized form (approximately 0.6 to 1.7 MDa) that is generated by hydrophobic clustering instead of covalent linkage. Electron microscopy (EM) and single-particle analysis showed that the multimerized VLRBs form globular-shaped clusters with an average diameter of 28.7 ± 2.2 nm. The presence of VLRBs in the complex was confirmed by immune-EM analysis using an anti-VLRB antibody. Furthermore, the hydrophobic hagfish C-terminus (HC) was capable of triggering multimerization and directing the cellular surface localization via a glycophosphatidylinositol linkage. Our results strongly suggest that the hagfish VLRB forms a previously unknown globular-shaped antibody. This novel identification of a structurally unusual VLRB complex may suggest that the adaptive immune system of hagfish differs from that of lamprey
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