12 research outputs found
Evaluation of Gymnema sylvestre Antimicrobial Activity in Methanol
G. sylvestre is a medicinal plant known for its sugar destroying property, as an anti-diabetic agent. The major phytoconstituents are the triterpenoid saponins, responsible for the various activities. The antimicrobial activity of this plant has been assessed in methanol as the solvent system for the extraction of active principles. The gram positive and gram negative organisms used in the study, have shown susceptibility towards the extracts, with the root extracts at acidic pH, showing higher activity. E. coli and E. cloacae were found to be the most sensitive and Pseudomonas aeruginosa, the resistant type of microorganisms, based on the results obtained from the zones of inhibition. The broad spectrum activity of the plant can be utilised in the development of new antimicrobial drugs
New tetrameric forms of the rotavirus NSP4 with antiparallel helices
Rotavirus nonstructural protein 4, the first viral enterotoxin to be identified, is a multidomain, multifunctional glycoprotein. Earlier, we reported a Ca2+-bound coiled-coil tetrameric structure of the diarrhea-inducing region of NSP4 from the rotavirus strains SA11 and I321 and a Ca2+-free pentameric structure from the rotavirus strain ST3, all with a parallel arrangement of alpha-helices. pH was found to determine the oligomeric state: a basic pH favoured a tetramer, whereas an acidic pH favoured a pentamer. Here, we report two novel forms of the coiled-coil region of NSP4 from the bovine rotavirus strains MF66 and NCDV. These crystallized at acidic pH, forming antiparallel coiled-coil tetrameric structures without any bound Ca2+ ion. Structural and mutational studies of the coiled-coil regions of NSP4 revealed that the nature of the residue at position 131 (Tyr/His) plays an important role in the observed structural diversity
The flexible C terminus of the rotavirus non-structural protein NSP4 is an important determinant of its biological properties
The rotavirus non-structural protein NSP4 functions as the viral enterotoxin and intracellular receptor for the double-layered particles (DLP). The full-length protein cannot be expressed and/or purified to homogeneity from bacterial or insect cells. However, a bacterially expressed and purified mutant lacking the N-terminal 72 aa(\triangle N72) was recently obtained from strains Hg18 and SA11 exhibiting approximately 17–20-, 150–200- and 13166–15800-fold lower (50% diarrhoea-inducing dose) values in suckling mice compared with that reported for the partially pure, full-length protein, a C-terminal M175I mutant and a synthetic peptide comprising aa 114– 135, respectively, suggesting the requirement for a unique conformation for optimal functions of the purified protein. The stretch of approximately 40 aa from the C terminus of the cytoplasmic tail of the endoplasmic reticulum-anchored NSP4 is highly flexible and exhibits high sequence variation compared with the other regions, the significance of which in diarrhoea induction remain unresolved. Here, it was shown that every amino acid substitution or deletion in the flexible C terminus resulted in altered conformation, multimerization, trypsin resistance and thioflavin T (ThT)binding, and affected DLP binding and the diarrhoea-inducing ability of the highly diarrhoeagenic SA11 and Hg18 N72 in suckling mice. These studies further revealed that high ThT fluorescence correlated with efficient diarrhoea induction, suggesting the importance of an optimal ThT-recognizable conformation in diarrhoea induction by purified NSP4. These results based on biological properties provide a possible conformational basis for understanding the influence of primary sequence variations on diarrhoea induction in newborn mice by purified NSP4s that cannot be explained by extensive sequence analyses
Genome-Wide Association Study for Major Biofuel Traits in Sorghum Using Minicore Collection
Background: Production of biofuels from lignocellulosic crop biomass is an alternative to reduce greenhouse
gas emissions. The biofuel production involves collecting biomass, breaking down cell wall components followed
by the conversion of sugars to ethanol. The lingo-cellulosic biomass comprises 40-50% cellulose, 20-30% hemicellulose,
and 10-25% lignin. Sorghum is a widely adapted energy crop for biofuel production. Biomass with low
lignin, high cellulose, and high hemicellulose contents are exploited to attain maximum biofuel production efficiency.
Resistance to lodging, pest, disease, and abiotic stresses related to cell wall components is well documented, and quantitative
trait loci were identified to understand these traits' genetic correlation. Selection for reduced lignin and increased
cellulose content in stover can increase the ethanol yield. The Genome-Wide Association Studies (GWAS) is a
complementary approach to evaluating the marker and phenotype associations among large diversity panels. Single nucleotide
polymorphisms were scanned to identify loci associated with the traits of interest. In this study, the GWAS
was performed on 245 sorghum minicore genotypes to analyze agronomic traits (days to 50%flowering, fresh biomass
yield, dry biomass yield) and cell wall components (cellulose, hemicellulose, and lignin). Further, in-silico validation
of the candidate genes was performed in a global gene expression data from large-scale RNA sequencing studies in
sorghum available in the NCBI GEO database was used.
Objective: The objectives of this study are to evaluate native variations in biofuel related agronomic traits and stalk
cell wall components and to identify significant SNPs or loci related to the cell wall components.
Methods: In this article, an association mapping panel, comprising of 245 sorghum minicore germplasm accessions,
was evaluated during two post rainy seasons of 2013 and 2014, and observations were recorded on the whole plot- for
days to 50% flowering, fresh biomass yield (tha-1), and dry biomass yield (tha-1). The biomass of sun-dried plants from
both seasons was collected separately, chopped, dried, and ground to powder. The cellulose, hemicellulose, and lignin
contents were determined in the powdered. The content of each of these three components in sorghum was expressed
in percent of dry matter. The data on agronomic traits and composition analysis was subjected to Analysis of Variance.
For the current study, we remapped the raw GBS data with the sorghum assembly version v3.1. A total of 27,589 SNPs
were obtained with a minor allele frequency (MAF) >1% and missing data <50%. The GWAS was performed in a single
minicore population using FarmCPU, in R software. The synteny positions of the identified significant SNPs between
sorghum and other model crop species viz., maize, switchgrass, and Arabidopsis were represented using CIRCOS
software for traits viz., dry biomass yield, cellulose, hemicellulose, and lignin. The transcriptome dataset from
where sorghum gene atlas studies of grain, sweet, and bioenergy sorghums are available through NCBI's Gene Expression
Omnibus (GEO) under accession number GSE49879, was used to cross-validate the identified SNPs for cellulose,
hemicellulose, and lignin through GWAS.
Results: High broad-sense heritability was exhibited for all the traits in individual seasons along with significant genotype
× environment interaction across seasons except lignin. Association mapping with a P < 1×10−4 revealed genomic
regions associated with the- (i) agronomic traits (days to 50% flowering, fresh and dry biomass), and (ii) biochemical
traits (cellulose, hemicellulose, and lignin) associated with biofuels production, in individual seasons. Twelve significant
SNPs for flowering time, 30 fresh biomass yields, and 24 for dry biomass yield, 25 for cellulose, 7 for hemicellulose,
and 21 for lignin were identified. CIRCOS plot was constructed to identify and analyze similarities and differences
while comparing the sorghum genome with different crops. For cellulose high similarity of >80% was observed
for all sorghum gene sequences with the maize homologs. The overall similarity of sorghum homologs with foxtail millet
was >65%, for Arabidopsis from 30.6% to 48.6%, and rice from 28.2% to 92.8%. SNPs for hemicellulose displayed
maximum similarity to foxtail millet followed by maize. The sequence similarity of lignin SNPs in sorghum was highest
with the maize genome followed by Arabidopsis. Both rice and foxtail millet showed >55% similarity to the
sorghum genome.
Conclusion: This study reports large variability for agronomic and biofuel traits in the sorghum minicore collection
with high heritability. The genetic architecture of cell wall components using the GWAS approach was studied and candidate
genes for each component were annotated. These results give a better understanding of the genetic basis of the
sorghum cell wall composition. The association analysis identified regions of the genome that could be targeted to enhance
the quality of biomass and yield along with the desired composition promoting breeding efficiency for enhanced
biofuel yield