Hypoglycemic effects of Lagenaria siceraria, Cynodon dactylon and Stevia rebaudiana extracts

Introduction: The aim of the current analysis was to judge the hypoglycemic action of the phyto-extracts of Lagenaria siceraria, Cynodon dactylon and Stevia rebaudiana using suitable in vitro approaches. Methods: The hypoglycemic activity of the phyto-material extracts was evaluated by employing various in-vitro methods namely glucose diffusion, amylolysis kinetics and glucose adsorption capacity. Results: The extracts of L. siceraria, C. dactylon and S. rebaudiana exhibited glucose dialysis retardation indices (GDRI) of 48.14%, 37.03% and 29.62%, respectively at 60 minutes which were reduced to 15.78%, 10.52% and 18.42%, respectively at 120 minutes. All the plant extracts used in the study adsorbed glucose and their adsorptions markedly enhanced with increase in sugar concentration. Conclusion: From the outcome of the assay it can be concluded that the extracts of L. siceraria, C. dactylon and S. rebaudiana have hypoglycemic activity as observed in various in-vitro assays. However, the beneficial actions require to be verified by adopting various in vivo techniques along with clinical trials for their efficient use as potential remedial moiety

Assay Method Development and Validation for Simultaneous Estimation of Mometasone Furoate and Azelastine HCL for Nasal Spray by RP-HPLC

A simple, precise, accurate, method was developed and validated for analysis of Mometasone Furoate and Azelastine Hydrochloride in nasal spray formulations. For development, different chromatographic condition and stress conditions like acid, base, peroxide, thermal and humidity as per ICH guidelines were used. Method was developed on reversed-phase C18 column using a mobile phase consisting of potassium dihydrogen, phosphate buffer and acetonitrile. Other HPLC parameters were flow rare 1 ml/min, detection wavelength 239 nm, injection volume 20 µl and column temperature 30°C. The developed method was further validated with respect to linearity, precision, accuracy, specificity and robustness. The results obtained were within the acceptance criteria as per ICH guidelines