Strategically examining the full-genome of dengue virus type 3 in clinical isolates reveals its mutation spectra

BACKGROUND: Previous studies presented the quasispecies spectrum of the envelope region of dengue virus type 3 (DENV-3) from either clinical specimens or field-caught mosquitoes. However, the extent of sequence variation among full genomic sequences of DENV within infected individuals remains largely unknown. RESULTS: Instead of arbitrarily choosing one genomic region in this study, the full genomic consensus sequences of six DENV-3 isolates were used to locate four genomic regions that had a higher potential of sequence heterogeneity at capsid-premembrane (C-prM), envelope (E), nonstructural protein 3 (NS3), and NS5. The extentof sequence heterogeneity revealed by clonal sequencing was genomic region-dependent, whereas the NS3 and NS5 had lower sequence heterogeneity than C-prM and E. Interestingly, the Phylogenetic Analysis by Maximum Likelihood program (PAML) analysis supported that the domain III of E region, the most heterogeneous region analyzed, was under the influence of positive selection. CONCLUSION: This study confirmed previous reports that the most heterogeneous region of the dengue viral genome resided at the envelope region, of which the domain III was under positive selection pressure. Further studies will need to address the influence of these mutations on the overall fitness in different hosts (i.e., mosquito and human) during dengue viral transmission

Asymptomatic ratio for seasonal H1N1 influenza infection among schoolchildren in Taiwan

Studies indicate that asymptomatic infections do indeed occur frequently for both seasonal and pandemic influenza, accounting for about one-third of influenza infections. Studies carried out during the 2009 pH1N1 pandemic have found significant antibody response against seasonal H1N1 and H3N2 vaccine strains in schoolchildren receiving only pandemic H1N1 monovalent vaccine, yet reported either no symptoms or only mild symptoms

Rationalisation and Validation of an Acrylamide-Free Procedure in Three-Dimensional Histological Imaging

201810_a bcmapublished_fina

Mimicking the Standard Model Higgs Boson in UMSSM

Motivated by the recent results in the standard model (SM) Higgs boson search at the Large Hadron Collider (LHC) we investigate the SM-like CP-even Higgs boson of the U(1)'-extended minimal supersymmetric standard model (UMSSM) and its branching ratio into the b b-bar, W W*, and \chi^0_1 \chi^0_1 modes. In the Summer 2011, a 2 sigma excess was reported in the channel H -> W W* -> l+ nu l- nu-bar around 130 - 140 GeV range. Later on in December 2011 announcements were made that an excess was seen in the 124-126 GeV range, while the SM Higgs boson above 131 GeV up to about 600 GeV is ruled out. We examine two scenarios of these mass ranges: (i) 130 GeV < M_{h_{SM-like}} < 141 GeV and show that the Higgs boson can decay into invisible neutralinos to evade the SM bound; and (ii) 120 GeV < M_{h_{\rm SM-like}} < 130 GeV and show that the Higgs boson can avoid decaying into neutralinos and thus gives enhanced rates into visible particles. We use the \eta model of E_6 with TeV scale supersymmetry to illustrate the idea by scanning the parameter space to realize these two different scenarios.Comment: 23 pages and 4 figure

Multiancestry analysis of the HLA locus in Alzheimer’s and Parkinson’s diseases uncovers a shared adaptive immune response mediated by HLA-DRB1*04 subtypes

Across multiancestry groups, we analyzed Human Leukocyte Antigen (HLA) associations in over 176,000 individuals with Parkinson’s disease (PD) and Alzheimer’s disease (AD) versus controls. We demonstrate that the two diseases share the same protective association at the HLA locus. HLA-specific fine-mapping showed that hierarchical protective effects of HLA-DRB1*04 subtypes best accounted for the association, strongest with HLA-DRB1*04:04 and HLA-DRB1*04:07, and intermediary with HLA-DRB1*04:01 and HLA-DRB1*04:03. The same signal was associated with decreased neurofibrillary tangles in postmortem brains and was associated with reduced tau levels in cerebrospinal fluid and to a lower extent with increased Aβ42. Protective HLA-DRB1*04 subtypes strongly bound the aggregation-prone tau PHF6 sequence, however only when acetylated at a lysine (K311), a common posttranslational modification central to tau aggregation. An HLA-DRB1*04-mediated adaptive immune response decreases PD and AD risks, potentially by acting against tau, offering the possibility of therapeutic avenues