Using multilevel regression mixture models to identify level-1 heterogeneity in level-2 effects

This article proposes a novel exploratory approach for assessing how the effects of Level-2 predictors differ across Level-1 units. Multilevel regression mixture models are used to identify latent classes at Level 1 that differ in the effect of 1 or more Level-2 predictors. Monte Carlo simulations are used to demonstrate the approach with different sample sizes and to demonstrate the consequences of constraining 1 of the random effects to 0. An application of the method to evaluate heterogeneity in the effects of classroom practices on students is used to show the types of research questions that can be answered with this method and the issues faced when estimating multilevel regression mixtures

MRI radiomic features are independently associated with overall survival in soft tissue sarcoma

Purpose: Soft tissue sarcomas (STS) represent a heterogeneous group of diseases, and selection of individualized treatments remains a challenge. The goal of this study was to determine whether radiomic features extracted from magnetic resonance (MR) images are independently associated with overall survival (OS) in STS. Methods and Materials: This study analyzed 2 independent cohorts of adult patients with stage II-III STS treated at center 1 (N = 165) and center 2 (N = 61). Thirty radiomic features were extracted from pretreatment T1-weighted contrast-enhanced MR images. Prognostic models for OS were derived on the center 1 cohort and validated on the center 2 cohort. Clinical-only (C), radiomics-only (R), and clinical and radiomics (C+R) penalized Cox models were constructed. Model performance was assessed using Harrell\u27s concordance index. Results: In the R model, tumor volume (hazard ratio [HR], 1.5) and 4 texture features (HR, 1.1-1.5) were selected. In the C+R model, both age (HR, 1.4) and grade (HR, 1.7) were selected along with 5 radiomic features. The adjusted c-indices of the 3 models ranged from 0.68 (C) to 0.74 (C+R) in the derivation cohort and 0.68 (R) to 0.78 (C+R) in the validation cohort. The radiomic features were independently associated with OS in the validation cohort after accounting for age and grade (HR, 2.4; Conclusions: This study found that radiomic features extracted from MR images are independently associated with OS when accounting for age and tumor grade. The overall predictive performance of 3-year OS using a model based on clinical and radiomic features was replicated in an independent cohort. Optimal models using clinical and radiomic features could improve personalized selection of therapy in patients with STS

The Velocity Dispersion of MS1054-03: A Massive Galaxy Cluster at High Redshift

We present results from a dynamical study of the high redshift, massive, X-ray luminous galaxy cluster MS1054--03. We significantly increase the number of confirmed cluster members by adding 20 to an existing set of twelve; using the confirmed members, we estimate MS1054--03's redshift, velocity dispersion, and mass. We find that z=0.8329 +/- 0.0017, sigma = 1170 +/- 150 km/s, and the central mass is approximately 1.9 +/- 0.5 x 10^{15} h^{-1} M_{odot} (within R=1 h^{-1} Mpc; H_0 =100h km s^{-1} Mpc^{-1}, q_0=0.5). MS1054--03 is one of a handful of high redshift (z>0.5) clusters known that also has X-ray and weak-lensing observations (Donahue et al. 1998; Luppino & Kaiser 1997); we find our dynamical mass agrees with mass estimates from both studies. The confirmation of MS1054--03 as a massive cluster at z~0.8 is consistent with an open (Omega_M~0.3) or flat, Lambda-dominated (Omega_M+Omega_{Lambda}=1) universe. In addition, we compare MS1054--03's velocity dispersion and X-ray temperature to a sample of low and intermediate redshift galaxy clusters to test for evolution in the sigma - T_x relation; we find no evidence for evolution in this relation to z~0.8.Comment: 13 pages, 3 figures, LaTex; Accepted for Publication in the Astrophysical Journa

The Structural Integrity of Anion Binding Exosite I of Thrombin Is Required and Sufficient for Timely Cleavage and Activation of Factor V and Factor VIII

Alpha-thrombin has two separate electropositive binding exosites (anion binding exosite I, ABE-I and anion binding exosite II, ABE-II) that are involved in substrate tethering necessary for efficient catalysis. Alpha-thrombin catalyzes the activation of factor V and factor VIII following discrete proteolytic cleavages. Requirement for both anion binding exosites of the enzyme has been suggested for the activation of both procofactors by alpha-thrombin. We have used plasma-derived alpha-thrombin, beta-thrombin (a thrombin molecule that has only ABE-II available), and a recombinant prothrombin molecule rMZ-II (R155A/R284A/R271A) that can only be cleaved at Arg(320) (resulting in an enzymatically active molecule that has only ABE-I exposed, rMZ-IIa) to ascertain the role of each exosite for procofactor activation. We have also employed a synthetic sulfated pentapeptide (DY(SO(3)(-))DY(SO(3)(-))Q, designated D5Q1,2) as an exosite-directed inhibitor of thrombin. The clotting time obtained with beta-thrombin was increased by approximately 8-fold, whereas rMZ-IIa was 4-fold less efficient in promoting clotting than alpha-thrombin under similar experimental conditions. Alpha-thrombin readily activated factor V following cleavages at Arg(709), Arg(1018), and Arg(1545) and factor VIII following proteolysis at Arg(372), Arg(740), and Arg(1689). Cleavage of both procofactors by alpha-thrombin was significantly inhibited by D5Q1,2. In contrast, beta-thrombin was unable to cleave factor V at Arg(1545) and factor VIII at both Arg(372) and Arg(1689). The former is required for light chain formation and expression of optimum factor Va cofactor activity, whereas the latter two cleavages are a prerequisite for expression of factor VIIIa cofactor activity. Beta-thrombin was found to cleave factor V at Arg(709) and factor VIII at Arg(740), albeit less efficiently than alpha-thrombin. The sulfated pentapeptide inhibited moderately both cleavages by beta-thrombin. Under similar experimental conditions, membrane-bound rMZ-IIa cleaved and activated both procofactor molecules. Activation of the two procofactors by membrane-bound rMZ-IIa was severely impaired by D5Q1,2. Overall the data demonstrate that ABE-I alone of alpha-thrombin can account for the interaction of both procofactors with alpha-thrombin resulting in their timely and efficient activation. Because formation of meizothrombin precedes that of alpha-thrombin, our findings also imply that meizothrombin may be the physiological activator of both procofactors in vivo in the presence of a procoagulant membrane surface during the early stages of coagulation

Novel critical exponent of magnetization curves near the ferromagnetic quantum phase transitions of Sr1-xAxRuO3 (A = Ca, La0.5Na0.5, and La)

We report a novel critical exponent delta=3/2 of magnetization curves M=H^{1/delta} near the ferromagnetic quantum phase transitions of Sr1-xAxRuO3 (A = Ca, La0.5Na0.5, and La), which the mean field theory of the Ginzburg-Landau-Wilson type fails to reproduce. The effect of dirty ferromagnetic spin fluctuations might be a key.Comment: 4 pages, 5 figure

Predatory Bacteria: A Potential Ally against Multidrug-Resistant Gram-Negative Pathogens

Multidrug-resistant (MDR) Gram-negative bacteria have emerged as a serious threat to human and animal health. Bdellovibrio spp. and Micavibrio spp. are Gram-negative bacteria that prey on other Gram-negative bacteria. In this study, the ability of Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus to prey on MDR Gram-negative clinical strains was examined. Although the potential use of predatory bacteria to attack MDR pathogens has been suggested, the data supporting these claims is lacking. By conducting predation experiments we have established that predatory bacteria have the capacity to attack clinical strains of a variety of ß-lactamase-producing, MDR Gram-negative bacteria. Our observations indicate that predatory bacteria maintained their ability to prey on MDR bacteria regardless of their antimicrobial resistance, hence, might be used as therapeutic agents where other antimicrobial drugs fail. © 2013 Kadouri et al

Resistivity scaling and critical dynamics of fully frustrated Josephson-junction arrays with on-site dissipation

We study the scaling behavior and critical dynamics of the resistive transition in Josephson-junction arrays, at f=1/2 flux quantum per plaquette, by numerical simulation of an on-site dissipation model for the dynamics. The results are compared with recent simulations using the resistively-shunted-junction model. For both models, we find that the resistivity scaling and critical dynamics of the phases are well described by the same critical temperature as for the chiral (vortex-lattice) transition, with a power-law divergent correlation length. The behavior is consistent with the single transition scenario, where phase and chiral variables order at the same temperature, but with different dynamic exponents z for phase coherence and chiral order.Comment: 17 pages, 13 figures, to appear in Phys. Rev.

Highly Resistive and Ultrafast Fe‐Ion Implanted InGaAs for the Applications of THz Photomixer and Photoconductive Switch

We develop highly‐resistive (i.e. low‐carrier‐density) and ultrafast Fe‐ion implanted InGaAs layers for the applications of THz photomixer and photoconductive switch. The measured Hall mobility, sheet resistance, carrier density, and carrier lifetime of the optimized 1.2‐μm‐thick Fe‐implanted InGaAs layer are 3.4×102 cm2/Vs, 0.24 MΩ, 6.5×1014 cm−3, and 0.13ps, respectively

Highly Resistive and Ultrafast Fe‐Ion Implanted InGaAs for the Applications of THz Photomixer and Photoconductive Switch

We develop highly‐resistive (i.e. low‐carrier‐density) and ultrafast Fe‐ion implanted InGaAs layers for the applications of THz photomixer and photoconductive switch. The measured Hall mobility, sheet resistance, carrier density, and carrier lifetime of the optimized 1.2‐μm‐thick Fe‐implanted InGaAs layer are 3.4×102 cm2/Vs, 0.24 MΩ, 6.5×1014 cm−3, and 0.13ps, respectively