A serpentine laminating micromixer combining splitting/recombination and advection

Mixing enhancement has drawn great attention from designers of micromixers, since the flow in a microchannel is usually characterized by a low Reynolds number ( Re) which makes the mixing quite a difficult task to accomplish. In this paper, a novel integrated efficient micromixer named serpentine laminating micromixer (SLM) has been designed, simulated, fabricated and fully characterized. In the SLM, a high level of efficient mixing can be achieved by combining two general chaotic mixing mechanisms: splitting/recombination and chaotic advection. The splitting and recombination ( in other terms, lamination) mechanism is obtained by the successive arrangement of "F''-shape mixing units in two layers. The advection is induced by the overall three-dimensional serpentine path of the microchannel. The SLM was realized by SU-8 photolithography, nickel electroplating, injection molding and thermal bonding. Mixing performance of the SLM was fully characterized numerically and experimentally. The numerical mixing simulations show that the advection acts favorably to realize the ideal vertical lamination of fluid flow. The mixing experiments based on an average mixing color intensity change of phenolphthalein show a high level of mixing performance was obtained with the SLM. Numerical and experimental results confirm that efficient mixing is successfully achieved from the SLM over the wide range of Re. Due to the simple and mass producible geometry of the efficient micromixer, SLM proposed in this study, the SLM can be easily applied to integrated microfluidic systems, such as micro-total-analysis-systems or lab-on-a-chip systems.X11159165sciescopu

Vulnerability assessment for the hazards of crosswinds when vehicles cross a bridge deck

AbstractA new procedure to assess the crosswind hazard of operating a vehicle over a bridge deck has been developed using a probabilistic approach that utilizes long-term wind data at bridge sites as well as the aerodynamic properties of bridge decks and vehicles. The proposed procedure for safety assessment considers the probabilities of two accident types: sideslip and overturning. The vulnerability of vehicles to crosswinds is represented by the number of days for traffic control that would be required to secure vehicle safety over a period of one year. The distribution of wind speed over a bridge deck was estimated from a section model wind tunnel test. A sea-crossing bridge was selected as an example, and a series of case studies were performed to identify the influential factors affecting vehicle vulnerability to crosswinds: vehicle type and loaded weight, the position of a running vehicle over a bridge deck, the bridge alignment relative to the dominant wind direction, and vehicle speed

Disposable Integrated Microfluidic Biochip for Blood Typing by Plastic Microinjection Moulding

Blood typing is the most important test for both transfusion recipients and blood donors. In this paper, a low cost disposable blood typing integrated microfluidic biochip has been designed, fabricated and characterized. In the biochip, flow splitting microchannels, chaotic micromixers, reaction microchambers and detection microfilters are fully integrated. The loaded sample blood can be divided by 2 or 4 equal volumes through the flow splitting microchannel so that one can perform 2 or 4 blood agglutination tests in parallel. For the purpose of obtaining efficient reaction of agglutinogens on red blood cells (RBCs) and agglutinins in serum, we incorporated a serpentine laminating micromixer into the biochip, which combines two chaotic mixing mechanisms of splitting/recombination and chaotic advection. Relatively large area reaction microchambers were also introduced for the sake of keeping the mixture of the sample blood and serum during the reaction time before filtering. The gradually decreasing multi-step detection microfilters were designed in order to effectively filter the reacted agglutinated RBCs, which show the corresponding blood group. To achieve the cost-effectiveness of the microfluidic biochip for disposability, the biochip was realized by the microinjection moulding of COC (cyclic olefin copolymer) and thermal bonding of two injection moulded COC substrates in mass production with a total fabrication time of less than 20 min. Mould inserts of the biochip for the microinjection moulding were fabricated by SU-8 photolithography and the subsequent nickel electroplating process. Human blood groups of A, B and AB have been successfully determined with the naked eye, with 3 mu l of the whole sample bloods, by means of the fabricated biochip within 3 min.X11100104sciescopu

Nitrogen Use Efficiency of Late Fall-Applied Urea and Pig Slurry for Regrowth of Perennial Ryegrass Sward

Pig slurry is the most important organic resource in Korea, as estimated to be more than 15% of recycled animal manure. The use of pig slurry as an alternative organic fertilizer is the most viable recycling option as it is produced in large amount on pig farms that has usually less or not surface for cultivation of forage crops in Korea. Perennial grasses in grassland system regrow successively after harvests by cutting or grazing. The regrowth yield at each harvest would be a crucial determinant for the productivity of sward. During vegetative regrowth, soil mineral N and N reserves meet the N requirements for shoot regrowth. The aims of this study are to estimate the N use efficiency of urea and pig slurry applied at late fall in relation to the N availability for restoring organic reserves and constructing ultimate regrowth biomass during successive three cycle of regrowth of perennial ryegrass sward

S Nutrition Is Involved in Alleviation of Damage of Photosynthetic Organelles by Salt Stress in Kentucky Bluegrass (\u3cem\u3ePoa pratensis\u3c/em\u3e L.)

Salt-stress is considered as one of the major environmental factor limiting plant growth and productivity. It has been well reported that salt stress induce the reduction of stomatal density and number leading to poor gaseous exchange which resulted in decrease of photosynthesis is associated with inhibition of several enzymes related to the Calvin cycle such as RuBisCo. In addition, salt stress decreases photosynthetic pigments such as chlorophyll and carotenoid which has important role in photosynthesis. Sulfur (S) is one of six macronutrients needed for proper plant growth and development. In our previous work, we found that sulfur nutrition has significant role in ameliorating the damaged in photosynthetic organelles caused by Fe-deficiency in oilseed rape (Muneer et al., 2014). In addition, application of sulfur mitigated the adverse effects of heavy metals stress by enhancing plant growth, chlorophyll content and net photosynthetic rate. Despite extensive researches attempting to elucidate the interactions between external sulfur supplies and stress tolerance, to our knowledge, the responses of the photosynthetic mechanism to combined S deficiency and salt stress have not yet been fully investigated. In this study, therefore, we hypothesized that S nutrition affects photosynthetic organs to salt stress, so that may involve in alleviating negative impact of salt stress in Kentucky bluegrass. To test this hypothesis, the responses of photosynthetic parameters, thylakoid protein complexes and ion uptake were compared for 21 days of four S and salt stress combined treatments; sulfur sufficient without salt stress (+S/non-salt, control), present of sulfur with salt stress (+S/salt), sulfur deprivation without salt stress (-S/non-salt) and sulfur deprivation and salt stress (-S/salt)

Splenectomy is associated with an aggressive tumor growth pattern and altered host immunity in an orthotopic syngeneic murine pancreatic cancer model.

The purpose of this study was to investigate whether splenectomy influences the tumor growth and metastatic pattern in an orthotopic syngeneic murine pancreatic cancer model. Murine pancreatic cancer cells (PAN02) were subcutaneously injected into the flanks of nude mice. A small tumor fragment (3 mm2), harvested from a subcutaneous tumor. was orthotopically implanted in the tail of the pancreas of C57/BL6 mice without splenectomy (control group, n=15) or with simultaneous splenectomy (splenectomy group, n=15). Tumor growth and metastatic patterns were analyzed by laparotomy at 21 days after surgery. No tumor growth was found in 5 mice (33.3%) of the control group and 1 mouse (6.7%) of the splenectomy group (p=0.169). Tumor volume was significantly larger in splenectomy group (p=0.013). Peritoneal seeding was more frequently observed in the splenectomy group (11 (73.3%) vs. 4 (26.7%), p=0.011). There were no differences in the number of liver and kidney metastasis between the two groups. The ratios of tumor-infiltrating CD4+ to FoxP3+ and CD8+ to FoxP3+ were significantly higher in the control group compared to the splenectomy group (8.2 ± 9.3 vs. 2.4 ± 1.5, p=0.046; 2.5 ± 1.4 vs. 1.5 ± 0.4, p=0.031, respectively). Splenectomy enhanced tumor growth and peritoneal seeding in an orthotopic syngeneic murine pancreatic cancer mouse model. The ramification of these results are discussed for pancreatic cancer treatment

Simultaneous VLBI Astrometry of H2O and SiO Masers toward the Semiregular Variable R Crateris

We obtained, for the first time, astrometrically registered maps of the 22.2 GHz H2O and 42.8, 43.1, and 86.2 GHz SiO maser emission toward the semiregular b-type variable (SRb) R Crateris, at three epochs (2015 May 21, and 2016 January 7 and 26) using the Korean Very-long-baseline Interferometry Network. The SiO masers show a ring-like spatial structure, while the H2O maser shows a very asymmetric one-side outflow structure, which is located at the southern part of the ring-like SiO maser feature. We also found that the 86.2 GHz SiO maser spots are distributed in an inner region, compared to those of the 43.1 GHz SiO maser, which is different from all previously known distributions of the 86.2 GHz SiO masers in variable stars. The different distribution of the 86.2 GHz SiO maser seems to be related to the complex dynamics caused by the overtone pulsation mode of the SRb R Crateris. Furthermore, we estimated the position of the central star based on the ring fitting of the SiO masers, which is essential for interpreting the morphology and kinematics of a circumstellar envelope. The estimated stellar coordinate corresponds well to the position measured by Gaia