In situ nutrient assays of periphyton growth in a lowland Costa Rican stream

Nutrient limitation of primary production was experimentally assessed using an in situ bioassay technique in the Quebrada Salto, a third-order tropical stream draining the northern foothills of the Cordillera Central in Costa Rica. Bioassays employed artificial substrata enriched with nutrients that slowly diffuse through an agar-sand matrix (Pringle & Bowers, 1984). Multiple comparisons of regression coefficients, describing chlorophyll- a accrual through time for different nutrient treatments, revealed positive micronutrient effect(s). Micronutrient treatment combinations (Fe, B, Mn, Zn, Co, Mo, EDTA), supplemented with and without nitrate and phosphate, exhibited significantly greater chlorophyll- a accrual over all other treatments (P < 0.05), supporting over three times that of the control after 14-d of substratum colonization. Neither of the major nutrients (N or P) produced a significant stimulation, although the N treatment displayed ≃50% more chlorophyll- a than the control after 14-d. Similarly, Si, EDTA, and Si + N + P treatments did not exhibit chlorophyll- a response curves that were significantly different from the control. During the experiment, mean NH 4 -N and (NO 2 + NO 3 )-N concentrations in the Salto were 2.0 µM (28.6 µg · l −1 ) and 7.2 µM (100.2 µg · l −1 ), respectively. High concentrations of PO 4 -P ( = 2.0 µM; 60.9 µg · l −1 ) and TP ( = 3.0 µM; 94.0 µg · l −1 ) were also found, and consequently low molar N:P ratios = 4.7). Despite the potential for N limitation in the system, both N and P appear to be at growth saturating levels. This may be due to micronutrient limitation and/or light limitation of periphyton growth in densely shaded upstream portions of the stream.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42872/1/10750_2004_Article_BF00008489.pd

Climate change and Lithuanian roads: impacts, vulnerability and adaptation

eISSN 2029-7092In Lithuania, like in other countries, climate change causes and will cause changes in natural and anthropogenic environment. The entire transport sector will be impacted, influencing the way it plans, designs, constructs and maintains infrastructure in the future. Roads are already sensitive to current climate variability. If today’s extreme weather events become both more frequent and extreme, so too will the level of disruption that they cause. Thus, roads must be adapted to changing climate conditions. The aim is to ensure resilience, to ensure that roads remain open under extreme weather conditions. The easiest and the most effective economically way is to implement adaptation measures for the new or reconstructed roads. But the existing older roads should be adapted also. The steps required to improve and maintain resilience of roads are definition of climate projections, identification of key roads and their vulnerability, identification and research on technologies for adaptation, preparation of methodologies, establishment of field operational trials. The aim of the research is to review Lithuanian roads in the context of climate change and its consequences. Methodology: climate and associated data collection and review, initial prognoses of the change (in short, medium and long term perspective) of meteorological elements, vulnerability assessment of the study area and the roads. Results: initial recommendations for adaptation action planningGamtos mokslų fakultetasVilniaus universitetasVytauto Didžiojo universiteta

Effects of thermodynamical processes on radiocesium behavior in lake tapeliai / Termodinaminių procesų įtaka radiocezio elgsenai tapelių ežere

The article presents the results of the integrated study of radiocesium behavior in Lake Tapeliai using not only conventional data on radiocesium activity concentrations in lake water and sediments but also a complex data set on seasonal variations and vertical profiles of standard water variables. Radiocesium activity concentrations in lake water as well as the vertical structure of the water column considerably depend on the inflows of the colored waters from the swampy watershed of the lake enriched in radiocesium. The global fallouts are mainly responsible for radiocesium inventory in lake sediments, which reaches maximum in the upper part of the water column above the ~5.4-m depth. The maximum values of radiocesium inventories in lake sediments are consistent with the respective densities of its deposits in the nearest forest soils. The main process of sediment activation is the direct sorption of radiocesium onto the sediment surface. Sedimentation rates in the lake vary mainly in the range of 3.5–5 mm·a–1. Lake bottom feeding sources located mainly on the southern terrace as well as their related near-bottom flows reduce respective sedimentation and radiocesium inventories in the sediments. Santrauka Straipsnyje nagrinėjama radiocezio elgsena Tapelių ežere, naudojant ne tik radiocezio savitojo ir tūrinio aktyvumų duomenis, bet ir standartinius vertikaliuosius parametrus, tokius kaip temperatūra, elektros laidumai ir oksiduoto sluoksnio storis. Radiocezio aktyvumas ežero vandenyje priklauso nuo spalvoto pelkinio vandens, kuriame yra radiocezio, patekimo į ežerą. Radiocezio apkrova dugno nuosėdose yra didžiausia viršutinėje ežero dalyje, t. y. iki 5,4 m gylio. Sedimentacijos greitis svyruoja 3,5–5 mm·m–1. Ežero priedugniniai vandens šaltiniai daugiausia išsidėstę pietinėje ežero terasoje, jų srovės sumažina sedimentaciją ir radiocezio apkrovą dugno nuosėdose. Raktiniai žodžiai: dugno nuosėdos, ežeras, radiocezis, radiocezio apkrov

Abnormal gangliosides are localized in lipid rafts in Sanfilippo (MPS3a) mouse brain

Allogenic stem cell transplantation can reduce lysosomal storage of heparan sulfate-derived oligosaccharides by up to 27 % in Sanfilippo MPS3a brain, but does not reduce the abnormal storage of sialolactosylceramide (GM3) or improve neurological symptoms, suggesting that ganglioside storage is in a non-lysosomal compartment. To investigate this further we isolated the Triton X100-insoluble at 4 °C, lipid raft (LR) fraction from a sucrose-density gradient from cerebral hemispheres of a 7 month old mouse model of Sanfilippo MPS3a and age-matched control mouse brain. HPLC/MS/MS analysis revealed the expected enrichment of normal complex gangliosides, ceramides, galatosylceramides and sphingomyelin enrichment in this LR fraction. The abnormal HS-derived oligosaccharide storage material was in the Triton X100-soluble at 4 °C fractions (8–12),whereas both GM3 and sialo[GalNAc]lactosylceramide (GM2) were found exclusively in the LR fraction (fractions 3 and 4) and were >90 % C18:0 fatty acid, suggesting a neuronal origin. Further analysis also revealed a >threefold increase in the late-endosome marker bis (monoacylglycerol) phosphate (>70 % as C22:6/22:6-BMP) in non-LR fractions 8–12 whereas different forms of the proposed BMP precursor, phosphatidylglycerol (PG) were in both LR and non-LR fractions and were less elevated in MPS3a brain. Thus heparan sulfate-derived oligosaccharide storage is associated with abnormal lipid accumulation in both lysosomal (BMP) and non-lysosomal (GM3 and GM2) compartments.G. Dawson, M. Fuller, K. M. Helmsley, J. J. Hopwoo