Terminology of bioanalytical methods (IUPAC Recommendations 2018)

Recommendations are given concerning the terminology of methods of bioanalytical chemistry. With respect to dynamic development particularly in the analysis and investigation of biomacromolecules, terms related to bioanalytical samples, enzymatic methods, immunoanalytical methods, methods used in genomics and nucleic acid analysis, proteomics, metabolomics, glycomics, lipidomics, and biomolecules interaction studies are introduced

Terminology of bioanalytical methods (IUPAC Recommendations 2018)

free accessRecommendations are given concerning the terminology of methods of bioanalytical chemistry. With respect to dynamic development particularly in the analysis and investigation of biomacromolecules, terms related to bioanalytical samples, enzymatic methods, immunoanalytical methods, methods used in genomics and nucleic acid analysis, proteomics, metabolomics, glycomics, lipidomics, and biomolecules interaction studies are introduced.Peer reviewe

Analysis of therapeutic peptides in human urine by combination of capillary zone electrophoresis-electrospray mass spectrometry with preparative capillary isotachophoresis sample pretreatment

The presented study deals with the off-line coupling of preparative isotachophoresis (pITP) with on-line combination of capillary zone electrophoresis with electrospray mass spectrometric detection (CZE-ESI-MS) used for the analysis of therapeutic peptides (anserine, carnosine, and buserelin) in complex matrix (urine). Preparative capillary isotachophoresis, operating in a discontinuous fractionation mode in column-coupling configuration, served as a sample pretreatment technique to separation, and fractionation of mixture of therapeutic peptides present in urine at low concentration level. The fractions isolated by pITP procedure were subsequently analyzed by capillary zone electrophoresis with electrospray mass spectrometric detection. Acetic acid at 200 mmol L-1 concentration served as background electrolyte in CZE stage and it is compatible with MS detection in positive ionization mode. In pITP fractionation procedure, sodium cation (10 mmol L-1 concentration) as leading ion and beta-alanine as terminating ion (20 mmol L-1 concentration) were used. While using CZE-ESI-MS, the limits of detection were 0.18 mu g mL(-1) for carnosine, 0.17 mu g mL(-1) for anserine and 0.64 mu g mL(-1) for buserelin in water and 0.19 mu g mL(-1) for carnosine, 0.50 mu g mL(-1) for anserine and 0.74 mu g mL(-1) for buserelin in 10 times diluted urine, respectively. The cleaning power of pITP sample pretreatment was proved as the peptides provided the higher MS signals at lower concentration levels resulting from the minimized matrix effects. The quality of obtained MS/MS spectra was very good so that they can provide information about the structure of analytes, and they were used for verification of the analytes identities. The pITP pretreatment improved the detection limits of the analyzed therapeutic peptides at least 25 times compared to the CZE-ESI-MS itself. (C) 2011 Elsevier B.V. All rights reserved

Bioanalytical Chemistry

This chapter provides a terminology of bioanalytical chemistry in general and analysis of biomacromolecules in particular. The vocabulary given in this chapter is largely taken from Labuda et al. “Terminology of bioanalytical methods (IUPAC Recommendations 2018)”,1 which becomes the immediate source reference for definitions of terms in this chapter that are not otherwise attributed. Reference to secondary sources follow the entry as “see also:” Terms are taken from the IUPAC Recommendations published in 1994 covering mostly the analytical terminology related to body fluids, enzymology, and immunology.2 Selected terms related to bioanalysis are included within recommendations and reports devoted to the unit “katal”,3 biotechnology,4 clinical chemistry,5 toxicology,6,7 medicinal chemistry,8,9 proteomics,10 electrochemical biosensors,11,12 and physical organic chemistry.13 Definitions of some terms have been updated here with respect to new reports and considerations, and a number of new terms has been introduced particularly on the topics of “–omics”, DNA analysis and studies of the interactions between biomolecules. Terms from earlier IUPAC Recommendations that are replaced by ref. 1 are not otherwise referenced but can be found as references in ref. 1