Pneumatic preloaded scanning science launch latch system

A relatively simple system using a preloaded pneumatic piston latch with a pyrotechnic valve release was developed. The system was the only candidate that met all the imposed requirements utilizing reliable state-of-art components. The development of the latch system from its first use on Mariner '69 Mars Flyby Spacecraft through its most recent use on the Voyager Spacecraft that will fly to Jupiter and Saturn is reviewed

Zorg en Kwaliteit : van individu naar systeem, naar beide

Rede uitgesproken door  Prof.dr. J. Kievit ter gelegenheid van zijn afscheid als hoogleraar Kwaliteit van Zorg aan de Universiteit Leiden op maandag 11 september 2017LUMC / Geneeskund

Surgical adverse outcome reporting as part of routine clinical care

Analysis and support of clinical decision makin

Kwaliteit van zorg: over het “Wat?” en het “Hoe?”

Oratie uitgesproken door Prof.dr. J. Kievit bij de aanvaarding van het ambt van hoogleraar op het gebied van Kwaliteit van Zorg aan de Universiteit Leiden op vrijdag 14 juni 201

Comparison of monoclonal antibodies 17-1A and 323/A3: the influence of the affinity on tumour uptake and efficacy of radioimmunotherapy in human ovarian cancer xenografts.

The low-affinity monoclonal antibody (MAb) chimeric 17-1A(c-17-1A) and the high-affinity MAb mouse 323/A3 (m-323/A3) were used to study the effect of the MAb affinity on the tumour uptake and efficacy of radioimmunotherapy in nude mice bearing subcutaneously the human ovarian cancer xenografts FMa, OVCAR-3 and Ov.Pe. Both MAbs are directed against the same pancarcinoma glycoprotein. In vitro, the number of binding sites on tumour cells at 4 degrees C was similar for both MAbs, but m-323/A3 had an approximately 5-fold higher affinity (1.3-3.0x10(9) M-1) than c-17-1A (3.0-5.4x10(8) M-1). This difference in affinity was more extreme at 37 degrees C, when no binding of c-17-1A could be observed. MAb m-323/A3 completely blocked binding of c-17-1A to tumour cells, whereas the reverse was not observed. Immunohistochemistry showed a similar but more intense staining pattern of m-323/A3 in human ovarian cancer xenografts than of c-17-1A. In vivo, the blood clearance in non-tumour-bearing nude mice was similar for both MAbs with terminal half-lives of 71.4 h for m-323/A3 and 62.7 h for c-17-1A. MAb m-323/A3 targeted better to tumour tissue, but was more heterogeneously distributed than c-17-1A. The cumulative absorbed radiation dose delivered by m-323/A3 to tumour tissue was 2.5- to 4.7-fold higher than that delivered by c-17-1A. When mice were treated with equivalent radiation doses of 131(I)m-323/A3 and 131(I)c-17-1A, based on a correction for the immunoreactivity of the radiolabelled MAbs, m-323/A3 induced a better growth inhibition in two of the three xenografts. When the radiation doses were adjusted to obtain a similar amount of radiation in the tumour c-17-1A was more effective in tumour growth inhibition in all three xenografts