3 research outputs found
WaterâBased Conductive Ink Formulations for EnzymeâBased Wearable Biosensors
Abstract Herein, this work reports the first example of secondâgeneration wearable biosensor arrays based on a printed electrode technology involving a waterâbased graphite ink, for the simultaneous detection of lâlactate and dâglucose. The waterâbased graphite ink is deposited onto a flexible polyethylene terephthalate sheet, namely stencilâprinted graphite (SPG) electrodes, and further modified with [Os(bpy)2(Cl)(PVI)10] as an osmium redox polymer to shuttle the electrons from the redox center of lactate oxidase from Aerococcus viridans (LOx) and gluocose oxidase from Aspergillus niger (GOx). The proposed biosensor array exhibits a limit of detection as low as (9.0 ± 1.0) Ă 10â6 m for LOx/SPGâ[Os(bpy)2(Cl)(PVI)10] and (3.0 ± 0.5) Ă 10â6 m for GOx/SPGâ[Os(bpy)2(Cl)(PVI)10], a sensitivity as high as 1.32 ÎŒA mmâ1 for LOx/SPGâ[Os(bpy)2(Cl)(PVI)10] and 28.4 ÎŒA mmâ1 for GOx/SPGâ[Os(bpy)2(Cl)(PVI)10]. The technology is also selective when tested in buffer and artificial sweat and is endowed with an operational/storage stability of â80% of the initial signal retained after 20 days. Finally, the proposed array is integrated in a wristband and successfully tested for the continuous monitoring of lâlactate and dâglucose in a healthy volunteer during daily activity. This is foreseen as a realâtime wearable device for sportâmedicine and healthcare applications
Water-Based Conductive Ink Formulations for Enzyme-Based Wearable Biosensors
Herein, this work reports the first example of second-generation wearable
biosensor arrays based on a printed electrode technology involving a
water-based graphite ink, for the simultaneous detection of l-lactate and
d-glucose. The water-based graphite ink is deposited onto a flexible
polyethylene terephthalate sheet, namely stencil-printed graphite (SPG)
electrodes, and further modified with [Os(bpy)2(Cl)(PVI)10] as an osmium
redox polymer to shuttle the electrons from the redox center of lactate oxidase
from Aerococcus viridans (LOx) and gluocose oxidase from Aspergillus niger
(GOx). The proposed biosensor array exhibits a limit of detection as low as
(9.0 ± 1.0) Ă 10â6 m for LOx/SPG-[Os(bpy)2(Cl)(PVI)10] and (3.0 ± 0.5) Ă
10â6 m for GOx/SPG-[Os(bpy)2(Cl)(PVI)10], a sensitivity as high as 1.32 uA
mmâ1 for LOx/SPG-[Os(bpy)2(Cl)(PVI)10] and 28.4 uA mmâ1 for
GOx/SPG-[Os(bpy)2(Cl)(PVI)10]. The technology is also selective when tested
in buffer and artificial sweat and is endowed with an operational/storage
stability of â80% of the initial signal retained after 20 days. Finally, the
proposed array is integrated in a wristband and successfully tested for the
continuous monitoring of l-lactate and d-glucose in a healthy volunteer
during daily activity. This is foreseen as a real-time wearable device for
sport-medicine and healthcare applications