VIPACFood: valorização de subprodutos alimentares para desenvolvimento de filmes e revestimentos que visem aumentar a vida útil de alimentos

O projeto VIPACFood é o acrónimo de “Valorization of Industrial fruits by Products and algae biomass waste: Development of Active Coatings to extend Food shelf life and reduce food losses”. Trata-se de um projeto de 3 anos que teve início no dia 1 de Junho de 2017 e é financiado pelo ARIMNet2. O consórcio do projeto é formado por oito organizações de investigação e governamentais, abrangendo quatro países, Tunísia, Itália, Portugal e Espanha. O coordenador é a Doutora Khaoula Khwaldia (National Institute of Research and Physicochemical Analysis, Tunísia). O projeto visa desenvolver tecnologias pós-colheita seguras e acessíveis que conduzam à redução de perdas, à melhoria da segurança alimentar e prazo de validade, reduzindo a quantidade de embalagem usada para preservar os alimentos, das quais resultam benefícios sociais, ambientais e económicos. O projeto também tem o objetivo de valorizar subprodutos industriais de frutas e resíduos de biomassa de algas, extraindo componentes ativos e funcionais com elevado valor e pela conceção de novos produtos transformados com grande apelo, estabilidade e potencialidade de comercialização que pode ajudar na redução dos subprodutos e resíduos eliminados e terá um impacto positivo na sustentabilidade das indústrias de transformação. Além de aumentar a eficiência económica e aumentar a competitividade dos produtores locais e das pequenas e médias empresas, espera-se que o projeto tenha impacto ambiental e na saúde devido à valorização de subprodutos, à redução significativa dos resíduos alimentares e à melhoria da qualidade dos alimentos e vida útil dos mesmosO presente trabalho insere-se no projeto VIPACFood. Este projeto é financiado pelo ARIMNet2 (Coordination of Agricultural Research in the Mediterranean; 2014-2017), uma ação ERA-NET financiada pela União Europeia (7º Programa Quadro).N/

Citrus By-Products: Valuable Source of Bioactive Compounds for Food Applications

This article belongs to the Special Issue Antioxidant Compounds Recovered from Food Wastes.ReviewCitrus production produces about 15 million tons of by-products/waste worldwide every year. Due to their high content of bioactive compounds, several extraction techniques can be applied to obtain extracts rich in valuable compounds and further application into food applications. Distillation and solvent extraction continues to be the most used and applied extraction techniques, followed by newer techniques such as microwave-assisted extraction and pulsed electric field extraction. Although the composition of these extracts and essential oils directly depends on the edaphoclimatic conditions to which the fruit/plant was exposed, the main active compounds are D-limonene, carotenoids, and carbohydrates. Pectin, one of the most abundant carbohydrates present in Citrus peels, can be used as a biodegradable polymer to develop new food packaging, and the extracted bioactive compounds can be easily added directly or indirectly to foods to increase their shelf-life. One of the applications is their incorporation in active food packaging for microbiological and/or oxidation inhibition, prolonging foods’ shelf-life and, consequently, contributing to reducing food spoilage. This review highlights some of the most used and effective extraction techniques and the application of the obtained essential oils and extracts directly or indirectly (through active packaging) to foods.This paper was carried out under the VIPACFood project, funded by ARIMNet2 (Coordina tion of Agricultural Research in the Mediterranean; 2014–2017), an ERA-NET Action financed by the European Union under the Seventh Framework Programme, and by the Programa de Cooperación Interreg V-A España–Portugal (POCTEP) 2014–2020 (project 0377_IBERPHENOL_6_E). Mariana A. Andrade is grateful for her research grant (SFRH/BD/138730/2018) funded by the Foundation for Science and Technology (FCT). Cássia H. Barbosa is grateful for her research grant in the frame of the VIPACFood project (ARIMNET2/0003/2016) and the Foundation for Science and Technology (FCT), Portugal for the Ph.D. Grant 2021.08154.BD. This work was financially supported by the Mechanical Engineering and Resource Sustainability Center—MEtRICs, which is financed by national funds from the FCT/MCTES (UIDB/50006/2020, UIDB/04077/2020 and UIDP/04077/2020).info:eu-repo/semantics/publishedVersio

Preparation, Characterization and Effectiveness to Delay Lipid Oxidation in Almonds and Beef Meat

Funding Information: This study was carried out under the VIPACFood project, funded by ARIMNet2 (Coordination of Agricultural Research in the Mediterranean; 2014–2017), an ERA-NET Action financed by the European Union under the Seventh Framework Programme, and by the Programa de Cooperación Interreg V-A España–Portugal (POCTEP) 2014–2020 (project 0377_IBERPHENOL_6_E). Cássia H. Barbosa is grateful for her research grant in the frame of the VIPACFood project (ARIMNET2/0003/2016) and the Foundation for Science and Technology (FCT), Portugal, for the Ph.D. grant 2021.08154.BD. This research was also funded by PT national funds (FCT/MCTES, Fundação para a Ciência e Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) through grants UIDB/50006/2020 and UIDB/00211/2020. L. Barbosa-Pereira is grateful to the Spanish Ministry of Science, Innovation and Universities for her “Juan de la Cierva-Incorporación” grant (Agreement No. IJCI-2017-31665). Publisher Copyright: © 2023 by the authors.Low-density polyethylene-based packaging with 4% lemon extract (LDPE/4LE) and two polylactic-based (PLA) packaging materials with 4% and 6% lemon extract (PLA/PEG/4LE and PLA/6LE) were produced. O2 and water permeability tests were performed, the total and individual phenolic compounds content were measured, and the films’ antioxidant activities were determined. The films’ ability to delay lipid oxidation was tested in two model foods: almonds, packaged with LDPE/4LE, PLA/4LE and PLA/6LE for a maximum period of 60 days at 40 °C (accelerated assay); and beef meat, packaged with the PLA/6LE for a maximum period of 11 days at 4 °C. The LE improved the WVP in all of the active films by 33%, 20% and 60% for the LDPE/4LE, PLA/4LE and PLA/6LE films, respectively. At the end of 10 days, the migration of phenolic compounds through the PLA films was measured to be 142.27 and 114.9 μg/dm2 for the PLA/4LE and PLA/6LE films, respectively, and was significantly higher than phenolic compounds migration measured for the LDPE/4LE (15.97 μg/dm2). Naringenin, apigenin, ferulic acid, eriocitrin, hesperidin and 4-hydroxybenzoic acid were the main identified compounds in the PLA, but only 4-hydroxybenzoic acid, naringenin and p-coumaric acid were identified in the LDPE films. Regarding the films’ ability to delay lipid oxidation, LDPE/4LE presented the best results, showing a capacity to delay lipid oxidation in almonds for 30 days. When applied to raw beef meat, the PLA/6LE packaging was able to significantly inhibit lipid oxidation for 6 days, and successfully inhibited total microorganisms’ growth until the 8th day of storage.publishersversionpublishe

Mise au point, caractérisation et application d'un emballage biodégradable complexe à base de fibres cellulosiques (papier) et de protéines animales (caséinate de sodium)

Des dispersions de couchage composées de substances naturelles (mica, cire de carnauba, glycérol et caséinate de sodium) ont été préparées et ensuite déposées sur la surface poreuse du papier. L'influence de chaque constituant des dispersions de couchage sur les propriétés barrières à l'oxygène et à la vapeur d'eau et les propriétés mécaniques a été étudiée. L'utilisation de la méthode des surfaces de réponse permet de quantifier l'influence et les interactions des facteurs (concentration en mica, concentration en cire, concentration en glycérol et concentration en caséinate de sodium). La modélisation par méthode empirique des propriétés barrières à la vapeur d'eau et à l'oxygène et les propriétés mécaniques des papiers, utilise une matrice de Doehlert et conduit à l'optimisation des facteurs étudiés. Nos résultats ont montré que l'enduction à base de caséinate de sodium améliore considérablement les propriétés barrières à l'oxygène du papier. La perméabilité à l'oxygène des papiers couchés (10 g/m2) est 13 à 90 fois inférieure à celle des papiers non couchés (0 g/m1. En effet, l'enduit protéique rend la surface du papier plus lisse et plus homogène que la surface rugueuse du papier non enduit. L'enduit déposé sur la surface du papier améliore également la résistance mécanique et l'élongation du papier en remplissant les pores et les espaces interfibrillaires de la structure fibreuse du papier.The combined effects of mica (0-1.2% (w/w)), carnauba wax (0-0.8% (w/w)), glycerol (0-6% (w/w)) and sodium caseinate (10-13% (w/w)) concentrations on barrier and mechanical properties of coated paper were studied. ln the first part of this work, the use of Response Surface Methodology led to quantify the effects and the interactions of different variables (mica, carnauba wax, glycerol and sodium caséinate concentrations). An empirical model using a Doehlert matrix was developed to account for the overall interactions between parameters and allow selection of the most efficient monitoring parameters on a large scale. Results showed that coating significantly increased oxygen barrier property. Indeed, protein coating increased homogeneity and smoothness of the paper surface by filling the porous structure of the paper. The tensile strength and elongation of coated paper, having a 10 g.m-2 coating weight were greater than that ofuncoated paper, which means that coating increased paper strength and ductility.NANCY/VANDOEUVRE-INPL (545472102) / SudocSudocFranceF

Active food packaging films from alginate and date palm pit extract: Physicochemical properties, antioxidant capacity, and stability

Date palm pits are highly available and inexpensive palm date by-products, representing a valuable source of natural antioxidants, particularly phenolic compounds. Date palm pit extract (DPPE) was prepared from these waste products and characterized for its phenolic content and in vitro antioxidant activity. Profiling DPPE by liquid chromatography coupled with mass spectrometry (LC/MS) showed the presence of dimers and trimers of (epi)catechin as the main constituents. Alginate-based films with four increasing concentrations of DPPE (10%, 20%, 30%, and 40% w/w) were prepared by the casting method. DPPE incorporation reduced solubility values of alginate films by 37%–64% and their surface wettability by 72%–111%. The incorporation of 10% DPPE improved water vapor barrier properties and increased tensile strength (TS) and elongation at break (%E) of alginate films by more than 23%, 50%, and 45%, respectively. The film containing 40% DPPE showed the lowest loss of phenolic content (32%), DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activity (38%), and ferric reducing antioxidant power (FRAP) (30%) after storage for 3 months

Milk Proteins for Edible Films and Coatings

International audienc

Physical properties and antifungal activity of bioactive films containing Wickerhamomyces anomalus killer yeast and their application for preservation of oranges and control of postharvest green mould caused by Penicillium digitatum

This study assessed the ability of two bio-based films, obtained from sodium alginate (NaAlg) and locust beangum (LBG), to protect the viability of Wickerhamomyces anomalus cells and control the growth of Penicilliumdigitatum. The effect of microbial cell incorporation on physical properties of the developed films was evaluatedin terms of barrier, mechanical and optical properties. Furthermore, the application of these two matrices as bioactivecoatingswas investigated in order to evaluate their efficacy in preserving the postharvest quality of ‘Valencia’oranges and inhibiting the growth of P. digitatum on artificially inoculated fruits. Results showed that NaAlgand LBG films were able to maintain more than 85% of the initial W. anomalus yeast population and that the developedfilms incorporating the killer yeast completely inhibited the growth of P. digitatumin synthetic medium.Likewise, NaAlg and LBG coatings enriched with W. anomalus yeast were effective at reducing weight loss andmaintaining firmness of ‘Valencia’ oranges during storage, and reduced green mold in inoculated fruits bymore than 73% after 13 days

New paradigms of food Packaging [Novos paradigmas das embalagens alimentares]

In recent years, new food packaging materials and processing techniques have attracted attention from the scientific community, industry and consumers. Some of these new materials are obtained from renewable resources but do not present satisfactory mechanical and/or barrier properties, therefore solutions in order to improve their performance are required. Moreover multifunctional materials are preferable in order to meet the different requirements of the most exigent consumers. In this presentation two on-going projects (VIPACFood and iFILM) in the field of active food packaging are introduced and reviewed in terms of objectives, methodologies, expected results/impact and innovation potential. VIPACFood, the acronym of “Valorisation of Industrial fruits by Products and algae biomass waste: Development of Active Coatings to extend Food shelf life and reduce food losses”, is a 3 years (2017-2020) research project funded by ARIMNet2.The consortium is formed by eight partners from Tunisia (Coordinator: Dr. Khaoula Khwaldia), Portugal, Spain and Italy. The main objectives of the project are to valorize industrial fruits by-products and algae biomass waste, extracting active and functional components with high added value and formulating new food products. Moreover the project also aims to develop novel films and coatings incorporating value-added components. Besides enhancing economic efficiency and increase competitiveness of local producers and SMEs, it is expected that the project will have health and environmental impact due to the valorisation of byproducts, reduction of food waste and loss and enhancement of food quality and shelf life. The project iFILM – Multifunctional Films for Intelligent and Active Applications – is a national project funded by FEDER and Portugal 2020. The main goal of the project is the development of technology for the continuous production of ultra fine thermoplastic films laminated with functional surfaces. The project (2017-2020) consortium is formed by Periplast (promotor company), Lusiaves, National Institute of Health Dr Ricardo Jorge (INSA, I.P.) and Polytecnic Institute of Leiria. Acknowledgments: This work was carried out in the frame of the VIPACFood project. This project is funded by ARIMNet2 (Coordination of Agricultural Research in the Mediterranean; 2014-2017), an ERA-NET Action financed by the European Union under the Seventh Framework Programme. This work was also supported by the research project “i.FILM – Multifunctional Films for Intelligent and Active Applications” (nº 17921) cofounded by European Regional Development Fund (FEDER) through the Competitiveness and Internationalization Operational Program under the “Portugal 2020” Program, Call no. 33/SI/2015, Co-Promotion Projects). Mariana Andrade is grateful for her research grant (2016/iFILM/BM) in the frame of iFILM project.Este trabalho foi financiado pelo projeto de investigação “i.FILM- Multifunctional Films for Intelligent and Active Applications” (nº 17921), cofinanciado pelo Fundo Europeu de Desenvolvimento Regional (FEDER) através do Programa Operacional Competitividade e Internacionalização no âmbito do Programa “Portugal 2020” (Sistema de Incentivos à Investigação e Desenvolvimento Tecnológico (SI I&DT), Aviso nº 33/SI/2015, Projetos em CoPromoção). Mariana Andrade e João Reboleira agradecem a bolsa de investigação (2016/iFILM/BM) no âmbito do projeto iFILM. Adicionalmente, os investigadores MARE-IPLeiria agradecem o financiamento da Fundação para a Ciência e Tecnologia (FCT), através do programa estratégico UID/MAR/04292/2013 concedido ao MARE.N/

Production and Characterization of New Biosurfactants/Bioemulsifiers from <i>Pantoea alhagi</i> and Their Antioxidant, Antimicrobial and Anti-Biofilm Potentiality Evaluations

The present work aimed to develop rapid approach monitoring using a simple selective method based on a positive hemolysis test, oil spreading activity and emulsification index determinations. It is the first to describe production of biosurfactants (BS) by the endophytic Pantoea alhagi species. Results indicated that the new BS evidenced an E24 emulsification index of 82%. Fourier-transform infrared (FTIR) results mentioned that the described BS belong to the glycolipid family. Fatty acid profiles showed the predominance of methyl 2-hyroxydodecanoate in the cell membrane (67.00%) and methyl 14-methylhexadecanoate (12.05%). The major fatty acid in the BS was oleic acid (76.26%), followed by methyl 12-methyltetradecanoate (10.93%). Markedly, the BS produced by the Pantoea alhagi species exhibited antimicrobial and anti-biofilm activities against tested human pathogens. With superior antibacterial activity against Escherchia coli and Staphylococcus aureus, a high antifungal effect was given against Fusarium sp. with a diameter of zone of inhibition of 29.5 mm, 36 mm and 31 mm, obtained by BS dissolved in methanol extract. The DPPH assay indicated that the BS (2 mg/mL) showed a higher antioxidant activity (78.07 inhibition percentage). The new BS exhibited specific characteristics, encouraging their use in various industrial applications

Mechanical and barrier properties of sodium caseinate-anhydrous milk fat edible films

International audienc