Mitochondrial apoptosis induced by Chamaemelum nobile extract in breast cancer cells

Chamaemelum nobile (Asteraceae) commonly known as ‹Roman chamomile› is a medicinal plant used for numerous diseases in traditional medicine, although its anticancer activity is unknown. The present study was carried out to investigate the anticancer as well as apoptotic activity of ethyl acetate fraction of C. nobile on different cancerous cell lines. The cells were treated with varying concentrations (0.001-0.25 mg/mL) of this fraction for 24, 48 and 72 h. Apoptosis induced in MCF-7 cells following treatment with ethyl acetate fraction was measured using Annexin V/PI, flowcytometry and western blotting analysis. The results showed that C. nobile ethyl acetate fraction revealed relatively high antiproliferative activity on MCF-7 cells; however, it caused minimal growth inhibitory response in normal cells. The involvement of apoptosis as a major cause of the fraction-induced cell death was confirmed by annexin-V/PI assay. In addition, ethyl acetate fraction triggered the mitochondrial apoptotic pathway by decreasing the Bcl-2 as well as increasing of Bax protein expressions and subsequently increasing Bax/Bcl-2 ratio. Furthermore, decreased proliferation of MCF-7 cells in the presence of the fraction was associated with G2/M phase cell cycle arrest. These findings confirm that ethyl acetate fraction of C.nobile may contain a diversity of phytochemicals which suppress the proliferation of MCF-7 cells by inducing apoptosis. © 2016 by School of Pharmacy Shaheed Beheshti University of Medical Sciences and Health Services

NXSensor web tool for evaluating DNA for nucleosome exclusion sequences and accessibility to binding factors

Nucleosomes, a basic structural unit of eukaryotic chromatin, play a significant role in regulating gene expression. We have developed a web tool based on DNA sequences known from empirical and theoretical studies to influence DNA bending and flexibility, and to exclude nucleosomes. NXSensor (available at ) finds nucleosome exclusion sequences, evaluates their length and spacing, and computes an ‘accessibility score’ giving the proportion of base pairs likely to be nucleosome-free. Application of NXSensor to the promoter regions of housekeeping (HK) genes and those of tissue-specific (TS) genes revealed a significant difference between the two classes of gene, the former being significantly more open, on average, particularly near transcription start sites (TSSs). NXSensor should be a useful tool in assessing the likelihood of nucleosome formation in regions involved in gene regulation and other aspects of chromatin function

Entropy of 4D extremal black holes

We derive the Bekenstein-Hawking entropy formula for four-dimensional Reissner-Nordstr\"om extremal black holes in type II string theory. The derivation is performed in two separate (T-dual) weak coupling pictures. One uses a type IIB bound state problem of D5- and D1-branes, while the other uses a bound state problem of D0- and D4-branes with macroscopic fundamental type IIA strings. In both cases the D-brane systems are also bound to a Kaluza-Klein monopole, which then yields the four-dimensional black hole at strong coupling

Is string theory a theory of strings?

Recently a great deal of evidence has been found indicating that type IIA string theory compactified on K3 is equivalent to heterotic string theory compactified on T^4. Under the transformation which relates the two theories, the roles of fundamental and solitonic string solutions are interchanged. In this letter we show that there exists a solitonic membrane solution of the heterotic string theory which becomes a singular solution of the type IIA theory, and should therefore be interpreted as a fundamental membrane in the latter theory. We speculate upon the implications that the complete type IIA theory is a theory of membranes, as well as strings

Surface Crack Characterization: Geometry and Stress Intensity Factor Measurements

The reflection coefficient of a Rayleigh wave at a surface crack is measured in both the long wavelength and the short wavelength limits. from the long wavelength measurement, the maximum value of the stress intensity factor is evaluated. Using glass samples, we have formed surface cracks, measured their reflection coefficient, and predicted failure stress with an error of less than 5% from the measured fracture stress. In short wavelength limit, the reflection coefficient is measured versus frequency. Length and depth resonances of the crack are observed and used to estimate the crack geometry with an accuracy of 10-20% for EDM notches in steel

Total Widths And Slopes From Complex Regge Trajectories

Maximally complex Regge trajectories are introduced for which both Re $\alpha(s)$ and Im $\alpha(s)$ grow as $s^{1-\epsilon}$ ($\epsilon$ small and positive). Our expression reduces to the standard real linear form as the imaginary part (proportional to $\epsilon$) goes to zero. A scaling formula for the total widths emerges: $\Gamma_{TOT}/M\to$ constant for large M, in very good agreement with data for mesons and baryons. The unitarity corrections also enhance the space-like slopes from their time-like values, thereby resolving an old problem with the $\rho$ trajectory in $\pi N$ charge exchange. Finally, the unitarily enhanced intercept, $\alpha_{\rho}\approx 0.525$, \nolinebreak is in good accord with the Donnachie-Landshoff total cross section analysis.Comment: 9 pages, 3 Figure

Scattering of Four-Dimensional Black Holes

The moduli space metric for an arbitrary number of extremal black holes in four dimensions with arbitrary relatively supersymmetric charges is found.Comment: 16 pages, REVTeX; references adde

Infrared renormalons and analyticity structure in pQCD

Relation between the infrared renormalons, the Borel resummation prescriptions, and the analyticity structure of Green functions in perturbative QCD (pQCD) is investigated. A specific recently suggested Borel resummation prescription resulted in the Principal Value and an additional power-suppressed correction that is consistent with the Operator Product Expansion. Arguments requiring the finiteness of the result for any power coefficient of the leading infrared renormalon, and the consistency in the case of the absence of that renormalon, require that this prescription be modified. The apparently most natural modification leads to the result represented by the Principal Value. The analytic structure of the amplitude in the complex coupling plane, obtained in this way, is consistent with that obtained in the literature by other methods.Comment: 6 pages, revtex4, 1 eps-figure; improved version - the paragraph containing Eqs.(18) and (19) is new, as well as the next paragraph; the Title modified; some references added; version to appear in Phys. Rev.

Curvature-corrected dilatonic black holes and black hole -- string transition

We investigate extremal charged black hole solutions in the four-dimensional string frame Gauss-Bonnet gravity with the Maxwell field and the dilaton. Without curvature corrections, the extremal electrically charged dilatonic black holes have singular horizon and zero Bekenstein entropy. When the Gauss-Bonnet term is switched on, the horizon radius expands to a finite value provided curvature corrections are strong enough. Below a certain threshold value of the Gauss-Bonnet coupling the extremal black hole solutions cease to exist. Since decreasing Gauss-Bonnet coupling corresponds to decreasing string coupling $g_s$, the situation can tentatively be interpreted as classical indication on the black hole -- string transition. Previously the extremal dilaton black holes were studied in the Einstein-frame version of the Gauss-Bonnet gravity. Here we work in the string frame version of this theory with the S-duality symmetric dilaton function as required by the heterotic string theory.Comment: 14 pages, 2 figure

Rotating Black Holes which Saturate a Bogomol'nyi Bound

We construct and study the electrically charged, rotating black hole solution in heterotic string theory compactified on a $(10-D)$ dimensional torus. This black hole is characterized by its mass, angular momentum, and a $(36-2D)$ dimensional electric charge vector. One of the novel features of this solution is that for $D >5$, its extremal limit saturates the Bogomol'nyi bound. This is in contrast with the $D=4$ case where the rotating black hole solution develops a naked singularity before the Bogomol'nyi bound is reached. The extremal black holes can be superposed, and by taking a periodic array in $D>5$, one obtains effectively four dimensional solutions without naked singularities.Comment: 13 pages, no figure