Cathodic Stripping Voltammetric Determination of Cefadroxil in Pharmaceutical Preparations and in Blood Serum

Abstract An analytical method has been developed using hanging mercury drop electrode (HMDE) for the quantitative determination of antibacterial drug cefadroxil (CFL) from pharmaceutical preparations and blood serum. Cathodic adsorptive stripping voltammetry was carried out in hydrochloric acid (0.1M): methanol (80: 20 v/v) and potassium chloride (0.1M) as supporting electrolyte. The reduction wave was obtained within -700 to -800 mV. Linear calibration curve was within 1-50µg/mL with detection limit of 0.1µg/mL of cefadroxil. Relative standard deviation for inter and intra day analysis of CFL was within 1-2%. The number of additives present in pharmaceutical preparations did not interfere the determination of cefadroxil. The analysis of pharmaceutical preparations and blood serum after chemotherapy with cefadroxil indicated relative standard deviation (RSD) within 0.8-1.2% and 2.6-3.8% respectively. The satisfactory results were obtained for quality control of cefadroxil in pharmaceutical preparations and in blood serum

Capillary electrophoresis mass spectrometry-based saliva metabolomics identified oral, breast and pancreatic cancer-specific profiles

Saliva is a readily accessible and informative biofluid, making it ideal for the early detection of a wide range of diseases including cardiovascular, renal, and autoimmune diseases, viral and bacterial infections and, importantly, cancers. Saliva-based diagnostics, particularly those based on metabolomics technology, are emerging and offer a promising clinical strategy, characterizing the association between salivary analytes and a particular disease. Here, we conducted a comprehensive metabolite analysis of saliva samples obtained from 215 individuals (69 oral, 18 pancreatic and 30 breast cancer patients, 11 periodontal disease patients and 87 healthy controls) using capillary electrophoresis time-of-flight mass spectrometry (CE-TOF-MS). We identified 57 principal metabolites that can be used to accurately predict the probability of being affected by each individual disease. Although small but significant correlations were found between the known patient characteristics and the quantified metabolites, the profiles manifested relatively higher concentrations of most of the metabolites detected in all three cancers in comparison with those in people with periodontal disease and control subjects. This suggests that cancer-specific signatures are embedded in saliva metabolites. Multiple logistic regression models yielded high area under the receiver-operating characteristic curves (AUCs) to discriminate healthy controls from each disease. The AUCs were 0.865 for oral cancer, 0.973 for breast cancer, 0.993 for pancreatic cancer, and 0.969 for periodontal diseases. The accuracy of the models was also high, with cross-validation AUCs of 0.810, 0.881, 0.994, and 0.954, respectively. Quantitative information for these 57 metabolites and their combinations enable us to predict disease susceptibility. These metabolites are promising biomarkers for medical screening

Preparation and use of maize tassels’ activated carbon for the adsorption of phenolic compounds in environmental waste water samples

The determination and remediation of three phenolic compounds bisphenol A (BPA), ortho-nitrophenol (o-NTP), parachlorophenol (PCP) in wastewater is reported. The analysis of these molecules in wastewater was done using gas chromatography (GC) × GC time-of-flight mass spectrometry while activated carbon derived from maize tassel was used as an adsorbent. During the experimental procedures, the effect of various parameters such as initial concentration, pH of sample solution, eluent volume, and sample volume on the removal efficiency with respect to the three phenolic compounds was studied. The results showed that maize tassel produced activated carbon (MTAC) cartridge packed solid-phase extraction (SPE) system was able to remove the phenolic compounds effectively (90.84–98.49 %, 80.75–97.11 %, and 78.27–97.08 % for BPA, o-NTP, and PCP, respectively) . The MTAC cartridge packed SPE sorbent performance was compared to commercially produced C18 SPE cartridges and found to be comparable. All the parameters investigated were found to have a notable influence on the adsorption efficiency of the phenolic compounds from wastewaters at different magnitudes

Separation of Copper & Nickel Complexes of Meso & dl-Bis(salicylaldehyde)-stilbenediimine Using Solvent Extraction & Adsorption HPLC

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