Urinary Albumin and Interleukin-8 Levels are not Good Indicators of Ongoing Vesicoureteral Reflux in Children who have no Active Urinary Tract Infection

Introduction: Vesicoureteral reflux (VUR) is a risk factor for kidney scarring, hypertension and declining renal function. Standard diagnostic methods are invasive and can cause exposure to radiation and urinary tract infections (UTIs). We aimed to investigate urine albumin and interleukin-8 levels as markers of ongoing VUR and renal damage in children without UTIs. Methods: Random urine samples were collected from 51 children, including 16 children with VUR (group A), 17 children with resolved VUR (group B) and 18 normal children (group C). The diagnosis of VUR or resolved VUR was confirmed by voiding cystourethrogram (VCUG) or direct radionuclide cystography (DRNC). All children had normal kidney function and had no evidence of UTI in the preceding three months. Random urine specimens were assayed for albumin (Alb), creatinine (Cr) and interleukin-8 (IL-8) and mean values were compared by one way ANOVA. Results: In groups A and B, the mean age at first UTI was 31.7 ± 2.4 and 27 ± 2.0 months respectively. In group A, the mean duration between VUR diagnosis and study entrance was 30 ± 9.1 months. In group B, the mean duration between VUR diagnosis and recovery was 19.9 ± 1.3 months. Overall, 76.4% of affected children had bilateral VUR and 41.2% had severe VUR. There were no significant differences in urinary Alb, IL-8, Alb/Cr and IL-8/Cr between the three groups. Conclusion: The current study does not support the hypothesis that microalbuminuria or urinary IL-8 are good indicators of ongoing VUR and renal injury in children. Keywords: Children; Interleukin-8; Microalbuminuria; Vesicoureteral Reflux

The Perspective Matters! Multisensory Integration in Ego-Centric Reference Frames Determines Full-Body Ownership

Recent advances in experimental science have made it possible to investigate the perceptual processes involved in generating a sense of owning an entire body. This is achieved by full-body ownership illusions which make use of specific patterns of visual and somatic stimuli integration. Here we investigate the fundamental question of the reference frames used in the process of attributing an entire body to the self. We quantified the strength of the body-swap illusion in conditions where the participants were observing this artificial body from the perspective of the first or third person. Consistent results from subjective reports and physiological recordings show that the first person visual perspective is critical for the induction of this full-body ownership illusion. This demonstrates that the multisensory integration processes producing the sense of corporeal self operates in an ego-centric reference frame

Novel insights into the architecture and protein interaction network of yeast eIF3.

Translation initiation in eukaryotes is a multistep process requiring the orchestrated interaction of several eukaryotic initiation factors (eIFs). The largest of these factors, eIF3, forms the scaffold for other initiation factors, promoting their binding to the 40S ribosomal subunit. Biochemical and structural studies on eIF3 need highly pure eIF3. However, natively purified eIF3 comprise complexes containing other proteins such as eIF5. Therefore we have established in vitro reconstitution protocols for Saccharomyces cerevisiae eIF3 using its five recombinantly expressed and purified subunits. This reconstituted eIF3 complex (eIF3(rec)) exhibits the same size and activity as the natively purified eIF3 (eIF3(nat)). The homogeneity and stoichiometry of eIF3(rec) and eIF3(nat) were confirmed by analytical size exclusion chromatography, mass spectrometry, and multi-angle light scattering, demonstrating the presence of one copy of each subunit in the eIF3 complex. The reconstituted and native eIF3 complexes were compared by single-particle electron microscopy showing a high degree of structural conservation. The interaction network between eIF3 proteins was studied by means of limited proteolysis, analytical size exclusion chromatography, in vitro binding assays, and isothermal titration calorimetry, unveiling distinct protein domains and subcomplexes that are critical for the integrity of the protein network in yeast eIF3. Taken together, the data presented here provide a novel procedure to obtain highly pure yeast eIF3, suitable for biochemical and structural analysis, in addition to a detailed picture of the network of protein interactions within this complex

New records of the genus and species Regenpolipus madrasensis (Acari: Heterostigmata: Podapolipidae), the ectoparasite of carabid beetles from Iran

پس از جمع‏آوری تعدادی سوسک با نام علمی Anthia sexguttata (Fab.) (Col.; Carabidae) در اطراف شهرستان تایباد واقع در استان خراسان رضوی، کلنی کنه‏های پارازیت خانواده‌ی Podapolipidae (Acari; Heterostigmata) که زیر بالپوش‏های یکی از سوسک‏های میزبان بود، به نام Regenpolipus madrasensis Husband & Ramaraju, 2006 شناسایی شد. این جنس و گونه برای اولین‌بار از ایران گزارش می‏شود

The role of multiplier bounds in fuzzy data envelopment analysis

The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.The non-Archimedean epsilon ε is commonly considered as a lower bound for the dual input weights and output weights in multiplier data envelopment analysis (DEA) models. The amount of ε can be effectively used to differentiate between strongly and weakly efficient decision making units (DMUs). The problem of weak dominance particularly occurs when the reference set is fully or partially defined in terms of fuzzy numbers. In this paper, we propose a new four-step fuzzy DEA method to re-shape weakly efficient frontiers along with revisiting the efficiency score of DMUs in terms of perturbing the weakly efficient frontier. This approach eliminates the non-zero slacks in fuzzy DEA while keeping the strongly efficient frontiers unaltered. In comparing our proposed algorithm to an existing method in the recent literature we show three important flaws in their approach that our method addresses. Finally, we present a numerical example in banking with a combination of crisp and fuzzy data to illustrate the efficacy and advantages of the proposed approach

A Biallelic Variant of the RNA Exosome Gene, EXOSC4, Associated With Neurodevelopmental Defects Impairs RNA Exosome Function and Translation

The RNA exosome is an evolutionarily conserved complex required for both precise RNA processing and decay. Pathogenic variants in EXOSC genes, which encode structural subunits of this complex, are linked to several autosomal recessive disorders. Here, we describe a missense allele of the EXOSC4 gene that causes a collection of clinical features in two affected siblings. This missense variant (NM_019037.3: exon3:c.560T\u3eC) changes a leucine residue within a conserved region of EXOSC4 to proline (p.Leu187Pro). The two affected individuals show prenatal growth restriction, failure to thrive, global developmental delay, intracerebral and basal ganglia calcifications, and kidney failure. Homozygosity for the damaging variant was identified by exome sequencing with Sanger sequencing to confirm segregation. To explore the functional consequences of this amino acid change, we modeled EXOSC4-L187P in the corresponding budding yeast protein, Rrp41 (Rrp41-L187P). Cells that express Rrp41-L187P as the sole copy of the essential Rrp41 protein show growth defects. Steady-state levels of both Rrp41-L187P and EXOSC4-L187P are decreased compared to controls, and EXOSC4-L187P shows decreased copurification with other RNA exosome subunits. RNA exosome target transcripts accumulate in rrp41-L187P cells, including the 7S precursor of 5.8S rRNA. Polysome profiles show a decrease in actively translating ribosomes in rrp41-L187P cells as compared to control cells with the incorporation of 7S pre-rRNA into polysomes. This work adds EXOSC4 to the structural subunits of the RNA exosome that have been linked to human disease and defines foundational molecular defects that could contribute to the adverse phenotypes caused by EXOSC pathogenic variants