Ovarian cystadenofibromas: a three cases report

Ovarian cystadenofibromas is a benign ovarian tumor that typically affects women in their fifth decade. Its risk factors remain unknown. This case study report 3 cases of ovarian cystadenofibromas treated in our department. The patients are aged 21, 28 and 50 years-old. The clinical pictures were polymorphic but the pelvic ultrasound of the case patients showed cystic ovarian masses suspected of malignancy. Two patients underwent laparoscopic ovarian cystectomy, and the third one, aged 50, underwent laparoscopic adnexectomy. The anatomopathological study showed benign ovarian cystadenofibromas. The operating follow ups were simple. It represents a relatively rare tumor whose macroscopic aspect evokes ovarian cancer wrongly leading to an aggressive surgical attitude

Insights into the architecture and stoichiometry of Escherichia coli PepA•DNA complexes involved in transcriptional control and site-specific DNA recombination by atomic force microscopy

Multifunctional Aminopeptidase A (PepA) from Escherichia coli is involved in the control of two distinct DNA transaction processes: transcriptional repression of the carAB operon, encoding carbamoyl phosphate synthase and site-specific resolution of ColE1-type plasmid multimers. Both processes require communication at a distance along a DNA molecule and PepA is the major structural component of the nucleoprotein complexes that underlie this communication. Atomic Force Microscopy was used to analyze the architecture of PepA·carAB and PepA·cer site complexes. Contour length measurements, bending angle analyses and volume determinations demonstrate that the carP1 operator is foreshortened by ∼235 bp through wrapping around one PepA hexamer. The highly deformed part of the operator extends from slightly upstream of the –35 hexamer of the carP1 promoter to just downstream of the IHF-binding site, and comprises the binding sites for the PurR and RutR transcriptional regulators. This extreme remodeling of the carP1 control region provides a straightforward explanation for the strict requirement of PepA in the establishment of pyrimidine and purine-specific repression of carAB transcription. We further provide a direct physical proof that PepA is able to synapse two cer sites in direct repeat in a large interwrapped nucleoprotein complex, likely comprising two PepA hexamers

The protein–DNA contacts in RutR·carAB operator complexes

Pyrimidine-specific regulation of the upstream carP1 promoter of the carbamoylphosphate synthase operon of Escherichia coli requires numerous trans-acting factors: the allosteric transcription regulator RutR, the nucleoid-associated protein integration host factor, and the trigger enzymes aminopeptidase A and PyrH (UMP-kinase). RutR, a TetR family member, binds far upstream of carP1. Here, we establish a high-resolution contact map of RutR•carP1 complexes for backbone and base-specific contacts, analyze DNA bending, determine the DNA sequence specificity of RutR binding by saturation mutagenesis, demonstrate that uracil but not thymine is the physiologically relevant ligand that inhibits the DNA binding capacity of RutR and build a model of the RutR·operator DNA complex based on the crystal structures of RutR and of the DNA-bound family member QacR. Finally, we test the validity of this model with site-directed mutagenesis of the helix–turn–helix DNA binding motif and in vitro binding studies with the cognate purified mutant RutR proteins

Régulation par les pyrimidines de l'opéron carAB codant pour la carbamoylphosphate synthétase d'Escherichia coli :participation de PepA/CarP (aminopeptidase A) et PyrH (UMP kinase)

Doctorat en Sciencesinfo:eu-repo/semantics/nonPublishe

Recherches sur la notion de légitimité en droit international

Legitimacy - setting generally the problem of the "foundation" and the obligatory nature of the law - has always occupied a limited place in the legal analysis mainly because of its legally non - defined character. on the other hand, if, in the internal law, the problem of the legitimacy seems to find a solution in the existence of a higher organ liable to bring about the necessary meditations between the controls on legality and the appreciations on legitimacy, the situation is different in the international law in the sense that the states - main subjects law avail themselves of various legitimacy likely to lead to conflicts un case of need to appraise legal and political situations. This situation is manifest in the classic international law where legitimacy has always been considered as a property of the internal order of the states. But this apparent neutrality of the international law with regard to every model of legitimacy, is basically explained both by the appropriateness between legitimacy and legality, and the pre-eminence of the principle of effectiviness. Moreover, the changes which took place in the international society - materialized by the action of the newly independent states will break up this block of validity, by dissociating legitimacy and legality, and will thus call for a new legitimacy.Posant généralement le problème du "fondement" et du caractère obligatoire du droit, la légitimité a toujours occupé une place limitée dans l'analyse juridique, due en grande partie à son caractère juridiquement non défini. D'autre part, si en droit interne, le problème de la légitimité semble trouver une solution dans l'existence d'un organe supérieur, susceptible d'opérer les médiations nécessaires entre les contrôles en légalité et les appréciations en légitimité ; en droit international, la situation est différente, en ce sens que les états, principaux sujets, peuvent se prévaloir de légitimités diverses, susceptibles de déboucher sur des conflits a l'occasion de l'appréciation des situations juridiques et politiques. Cette situation est très manifeste en droit international classique ou la légitimité a toujours été perçue comme une propriété de l'ordre interne des états. Mais, cette apparente neutralité du droit international à l'égard de tout modèle de légitimité, s'explique essentiellement par l'adéquation entre légitimité et légalité, et par la prééminence du principe d'effectivité. Aussi, les changements survenus dans la société internationale et matérialisés par l'action des états nouvellement indépendants, vont faire éclater ce bloc de validité en dissociant légitimité et légalité et déboucher sur l'exigence d'une nouvelle légitimité. Cette tendance est manifeste également par l'émergence de nouveaux principes de légitimité, appelant une traduction sur le plan de la légalité internationale et une protection juridique renforcée dans l'ordre international

Multidisciplinary Approach to Cover an Apex-Exposed Tooth: A Case Report after 6-Year Follow-Up

Introduction. The prognosis for a successful treatment of gingival recessions (GRs) is one of the main criteria for deciding whether or not and how to perform root coverage surgery. The defect-related factors are the most important to predict root coverage outcomes. Thus, severe GR could make the root coverage (RC) challenging especially in cases with advanced interdental clinical attachment loss (ICAL). Case Presentation. This case report demonstrates a challenging management of a deep localized Miller Class III GR with root apex exposure associated with ICAL. After initial therapy, the treatment had consisted of a multidisciplinary approach involving endodontic treatment, periodontal plastic surgery including a laterally positioned flap, and orthodontic treatment. The 6-year follow-up showed improvement in clinical outcomes (recession reduction (RR) and keratinized tissue (KT) augmentation) and a higher patient satisfaction. Conclusions. This case report demonstrates the role of the multidisciplinary approach in the management of deep GRs associated with ICAL. A rational choice of the RC technique was critical to achieve good clinical outcomes

Zebrafish as a Model to Study Vascular Elastic Fibers and Associated Pathologies

Many extensible tissues such as skin, lungs, and blood vessels require elasticity to function properly. The recoil of elastic energy stored during a stretching phase is provided by elastic fibers, which are mostly composed of elastin and fibrillin-rich microfibrils. In arteries, the lack of elastic fibers leads to a weakening of the vessel wall with an increased risk to develop cardiovascular defects such as stenosis, aneurysms, and dissections. The development of new therapeutic molecules involves preliminary tests in animal models that recapitulate the disease and whose response to drugs should be as close as possible to that of humans. Due to its superior in vivo imaging possibilities and the broad tool kit for forward and reverse genetics, the zebrafish has become an important model organism to study human pathologies. Moreover, it is particularly adapted to large scale studies, making it an attractive model in particular for the first steps of investigations. In this review, we discuss the relevance of the zebrafish model for the study of elastic fiber-related vascular pathologies. We evidence zebrafish as a compelling alternative to conventional mouse models