Occurrence of Vibrio spp., Aeromonas hydrophila, Escherichia coli and Campylobacter spp. in crayfish (Astacus leptodactylus) from Iran

The aim of this research was to study the occurrence of Vibrio spp., Aeromonas hydrophila, Escherichia coli and Campylobacter spp. in crayfish from Azerbaijan Province using culture method and PCR assay. A total of 55 isolates were collected from 97 studied samples. Vibrio spp., A. hydrophila, E. coli and Campylobacter spp. were detected in 26 samples (26.8%), 12 samples (12.3%), 15 samples (15.46%) and 2 samples (2.06%), respectively. Among Vibrio isolates, Vibrio vulnificus (11.3%) was the species most frequently detected followed by V. harveyi (7.2%), V. alginolyticus (2.06%) and V. mimicus (1.03%). The results of this study indicated that crayfish from the studied area contain pathogens relevant to public health

Exploring conserved mRNA-miRNA interactions in colon and lung cancers

Aim: The main goal of this analysis was prioritization of co-expressed genes and miRNAs that are thought to have important influences in the pathogenesis of colon and lung cancers. Background: MicroRNAs (miRNAs) as small and endogenous noncoding RNAs which regulate gene expression by repressing mRNA translation or decreasing stability of mRNAs; they have proven pivotal roles in different types of cancers. Accumulating evidence indicates the role of miRNAs in a wide range of biological processes from oncogenesis and tumor suppressors to contribution to tumor progression. Colon and lung cancers are frequently encountered challenging types of cancers; therefore, exploring trade-off among underlying biological units such as miRNA with mRNAs will probably lead to identification of promising biomarkers involved in these malignancies. Methods: Colon cancer and lung cancer expression data were downloaded from Firehose and TCGA databases and varied genes extracted by DCGL software were subjected to build two gene regulatory networks by parmigene R package. Afterwards, a network-driven integrative analysis was performed to explore prognosticates genes, miRNAs and underlying pathways. Results: A total of 192 differentially expressed miRNAs and their target genes within gene regulatory networks were derived by ARACNE algorithm. BTF3, TP53, MYC, CALR, NEM2, miR-29b-3p and miR-145 were identified as bottleneck nodes and enriched via biological gene ontology (GO) terms and pathways chiefly in biosynthesis and signaling pathways by further screening. Conclusion: Our study uncovered correlated alterations in gene expression that may relate with colon and lung cancers and highlighted the potent common biomarker candidates for the two diseases

Clinical and pathological characteristics of colorectal polyps in Iranian population

Background & Aim: Colon polyps are important lesions and a concern because of the potential for colorectal cancer. Colorectal carcinoma is one of the most common causes of cancer-related deaths in Iranian population. The distribution of polyps in the colon may affect the efficacy of a screening modality. The aim of this study was to determine clinical & pathology characteristics of colorectal polyps in Iranian population.Material & Methods: In this study is cross sectional survey that 856 polypectomies were done in 716 patients. Anatomical distribution, size and histopathology of polyps were described in 2004 – 2008 in the educational hospital of Taleghani in Tehran.Results: polyps were observed in 437 males and 279 females and gastroenterologists did colonoscopic polypectomy. 3.12 percent of polyps were located in the rectum, 19.6 percent in sigmoid, 24.4 percent in descending colon, 13.9 percent in transverse colon, 29.6 percent were in cecum and ascending colon..77(9%) polyps were non-neoplastic and 779(91%) were neoplastic. Adenomas were present in 727 (85%) cases, of these 411 (56% of adenomas) were left-sided and 316(44% of adenomas) were right-sided. Carcinoma was observed in 52 cases, of these 18(34.5%) were left sided and 34(65.5% of carcinomas) were right sided. Of them 354 were advance polyp (> 1cm, villous type, high grade dysplasia). 87(34%) of Advance polyps were in under 50 year patients and 149 (58.6 %) were right sided.Conclusion: This study has shown that a significant number of adenomas and carcinomas lie proximal to the splenic flexure. Thus, in the absence of left-sided lesions, it is expected that examination of the colon limited to the splenic flexure would miss 44% of such lesions. The increasing right-sided prevalence of these lesions with age suggests that evaluation of the proximal bowel is particularly important in older people. In addition there were higher stages of dysplasia and malignancy in larger polyps