8 weeks’ voluntary exercise training reduces apoptosis in hypothalamus of control, 3xtg-AD and 3xtg-AD+Ex groups.

<p>7A. Immunofluorescence of apoptotic positive cells of hypothalamic sections from the control, 3xtg-AD and 3xtg-AD Ex groups at 16 weeks of age. 7B. Quantification of apoptotic positive cells in the medio-basal hypothalamus among three groups. N = 3–5 per group. White arrows indicate apoptotic cell in the medio-basal hypothalamus. *p < 0.05 vs control group, ^#p < 0.05 vs 3xtg-AD group. 3V: third ventricle, scale bars represent 200 μM. Data represent mean ± SEM.</p

6 weeks’ voluntary exercise training improves glucose metabolism in triple transgenic 3xtg-AD mice.

<p>4A. Fasted and 4B. fed glucose levels at the age of 15 weeks, *p < 0.05 vs control group. 4C. Glucose tolerance test was performed in the control, 3xtg AD, and 3xtg AD + Ex groups at the age of 14 (n = 6), *p < 0.05 vs control group, #p < 0.05 vs 3xtg AD group.</p

POMC neuron populations after an 8-week voluntary exercise treatment among control, 3xtg-AD, and 3xtg-AD Ex groups.

<p>5A. Representative images of POMC neuron population, 5B. Quantification of POMC neuron populations after 8 weeks of voluntary exercise treatment among control, 3xtg-AD, and 3xtg-AD+Ex group. N = 3–5 per group. *p < 0.05 vs control group, ^#p < 0.05 vs 3xtg-AD group. 3V: third ventricle, scale bars represent 200 μM, Bregma -1.46mm. Data represent mean ± SEM.</p

Gene expression in the hypothalamus at 12 weeks after a 4-week voluntary exercise treatment in the triple transgenic (3xtg-AD) and control.

<p>3A. mRNA expression of AgRP, 3B. POMC, 3C MC4R, 3D. TNF-α, 3E. IL-6, 3F. IKKβ, 3G. PGC1 α, 3H. TFAM, 3J. NRF1 at 12 weeks among control, 3xtg-AD, and a 3xtg-AD group given a voluntary exercise treatment. N = 4–6 per group, *p< 0.05 vs Control, #p < 0.05 vs 3xtg-AD group. Data represent mean ± SEM.</p

Metabolic status at different age points (10, 12, and 24 weeks) in the triple transgenic (3xtg-AD) and control.

<p>2A. Food intake, 2B. Oxygen consumption, 2C. Fat mass, 2D. Lean mass, 2E. Running distance per day, 2F. Body weight at 10, 12, and 24 weeks between groups. N = 4–6 per group, *p< 0.05 vs Control. Data represent mean ± SEM.</p

Creation of a publication plan in the field of science and research

This thesis focuses on the topic of open data, especially in the field of science and research. The work is defined by six sub-targets and characterize the area of science and research in the Czech Republic and abroad, to characterize the issue of open data in the Czech Republic and in the world, describe the method of publication of data on the promotion of science and research in the Czech Republic and in the world, how to propose a procedure and create publications plan for TA CR, prepare a sample dataset, and then evaluate the procedure used. The work is divided into thematic blocks, which are based on these goals. It consists of an introduction, theoretical part, practical part and conclusion. The introductory section defines the objectives of work and research resources devoted to similar topics. The theoretical part describes the problems of science and the problem of open data in the Czech Republic and other countries, and also the process of creating publication plan according to the chosen methodology. The practical part contains according to the targets two parts, one is the analysis of open data of science and research and other preparation publication plan TA CR. The practical part ends with evaluation of procedure used with proposals for improvement. The conclusion contains a summary of the work and evaluate the goals achieved

Additional file 2: of Molecular characterization of irinotecan (SN-38) resistant human breast cancer cell lines

32 genes that are differentially expressed in both the MDA-MB-231 and the MCF7 model system. (XLSX 4123 kb