Model Tikus Omenn Syndrome

In 1965, Gilbert Omenn described a rare, inherited disorder which produced paradoxical combination of immunodeficiency and immune dysregulation. The term Omenn Syndrome was coined originating from this report. More than 40 years ago since it first described, the pathogenesis of OS has remained mysterious and these could be due to unavailable animal model for OS yet. This paper evaluates and discusses 2 murine models of OS that have been found and developed recently by 2 research groups from Japan and Italia. These two murine models bearing mutation in the V(D)J recombination analogous to those causing human OS. These two mouse models have been recapitulate the disease and provide insight into the pathogenesis of OS and also it relation with immunodeficiency combined with autoimmunity and atopy in OS and other related diseases

PERAN PSIKOTERAPI PSIKOANALIS DALAM PERSPEKTIF BUDDHIS

Jurnal ini membahas peran psikoterapi psikoanalis dalam perspektif Buddhis dengan tujuan menjelajahi potensi integrasi dua pendekatan yang berbeda ini terhadap pemahaman mendalam tentang kondisi batin manusia dan proses penyembuhan. Psikoterapi psikoanalis, yang memusatkan perhatiannya pada pemahaman terhadap alam bawah sadar dan dinamika psikologis, diintegrasikan dengan pandangan Buddhisme yang menekankan pemahaman mendalam tentang keterikatan, penderitaan, dan jalan menuju pembebasan. Melalui analisis literatur dan penerapan prinsip-prinsip psikoanalisis dalam konteks Buddhisme, penelitian ini menjelajahi cara di mana konsep-konsep seperti id, ego, dan superego dapat diterjemahkan dan diperkaya oleh perspektif Buddhisme. Integrasi ini membuka diskusi tentang bagaimana pemahaman tentang kondisi batin manusia, pengungkapan emosi, dan transformasi diri dapat ditingkatkan melalui penerapan prinsip-prinsip psikoterapi psikoanalis dalam praktik Buddhisme. Hasil penelitian ini diharapkan dapat memberikan wawasan baru tentang potensi kolaborasi antara psikoterapi psikoanalis dan Buddhisme, menawarkan pendekatan holistik yang menyelaraskan aspek-aspek psikologis dan spiritual dalam pemahaman dan penyembuhan kondisi batin. Implikasi praktis dan etis dari integrasi ini juga diperdebatkan, mengilustrasikan cara pendekatan terpadu ini dapat memberikan kontribusi pada pengembangan metode intervensi yang lebih komprehensif dan efektif

Effect of Steamed Broccoli Juice (Brassica Oleraceae L. Var. Italica) to the Serum Interleukin 8 Level in Colitis Murine Model

Background: Ulcerative colitis (UC) is a bowel inflammation that happens in the colon and rectum. In UC there is an increased level of inflammatory mediators, one of which is interleukin-8 (IL-8), the main chemoattractant for polymorphonuclear (PMN) inflammatory cells causing inflammation to worsen. Broccoli (Brassica oleraceae L. var. italica) contains sulforaphane which can inhibit nuclear factor kappa B (NF-ĸB), resulting in the decrease of IL-8. This study was performed to identify the effect of steamed broccoli juice to the serum interleukin-8 level in murine model of colitis.Method: This study was a true experimental laboratory study using complete randomized design. Experimental animals being used were 28 murines with Balb/c strain and were divided into 4 intervention groups. Serum IL-8 level was measured by using enzyme-linked immunosorbent assay (ELISA) method. Data analysis was performed using analysis of variance (ANOVA) test, followed by multiple comparisons Tukey honest significant difference (HSD) test (a = 0.05) and p 0.05.Results: The highest mean of IL-8 level was found in the positive control group. This study also showed highly significant difference (p < 0.001) of IL-8 level between positive control group and treatment group.Conclusion: Steamed broccoli juice could decrease serum interleukin-8 level in murine model of colitis

Comparison of the Effects of Three Extract Sorts of Soybean DETAM 1 on the Total Cholesterol Level in Balb-C Male Mice

BACKGROUND: Hypercholesterolemia is a major risk factor for coronary heart disease. Pharmacological treatment of  hypercholesterolemia are known to have side effects, hence the need for an alternative medicine, like herbal medicine, such as soybean as an example. Research objective is to compare the effects of ethanol extract, protein extract, and ethyl acetate fraction of soybean tempeh Detam-1 on the total serum cholesterol level of male mice. METHODS: This study was a laboratory experimental study with Completely Randomized Design (CRD) method. Twenty five balb-C strain male mice were allocated into five groups, namely: the negative-control (aquabidest), the positive-control (simvastatin), ethanol extract, protein extract and ethyl acetate fraction of the soybean tempeh Detam-1 and all were fed with high cholesterol containing food for 31 days. Starting on the 18th day all groups were treated for 14 days. The observed parameter was the total serum cholesterol level. The data of the study results were analyzed with ANOVA, followed by Tukey HSD test. RESULTS: There were significant differences between treatment groups and the negative-control (p=0.004). The reduction of the total cholesterol level of soy ethanol extract was 35.11±15%, the ethyl acetate fraction of soybean tempeh 26.48±17%, and soy protein extract was 15.33±33.1%, and no significant difference was noted in the positive-control group (simvastatin). CONCLUSIONS: The ethanol extract of soybean seed, protein extract of soybean seed, and ethyl acetate fraction of soybean tempeh Detam-1 have the same effect as do simvastin, and ethanol extract of soybean seed is the most effective substance for lowering total serum cholesterol in Balb-C male mice. KEYWORDS: hypericholesterolemia, ethanol extract, protein extract and ethyl acetate fraction, soybean Detam-1

Hypoxia in Mesenchymal Stem Cell

Mesenchymal stem cells (MSCs) are non-hematopoietic multipotent stem cells with self-renewal properties and ability to differentiate into a variety of mesenchymal tissues. This chapter overviews effects of hypoxia on MSCs, makes it promising therapy to various diseases. Cultivation of MSCs under hypoxic condition results in variety of outcome that is important to be noted in clinical use. In most studies, hypoxic condition appears to increase proliferation, differentiation, and immune regulatory performance of MSCs without affecting its characteristic. Those benefits are therefore utilized in clinical application. However, there are also studies that report on negative effects of hypoxia in MSCs such as chromosomal instability. Molecular mechanism of MSCs in hypoxic condition is provided for better understanding, which is crucial for further development with better outcome

SISTEM UBIQUITIN-PROTEASOM DAN HUBUNGANNYA DENGAN KARSINOGENESIS YANG DIINDUKSI OLEH PAPILLOMAVIRUS MANUSIA

Certain types of human papillomaviruses (HPV) have been etiologically associated with malignant lesion, most notably with cervical cancer. The major oncoproteins of these cancerassociated viruses are encoded by the viral E6 and E7 genes and characterization of these oncoproteins has shown that both E6 and E7 exploit the ubiquitin-proteasome system to degrade the cellular proteins