Synthesis, Jahn-Teller labeled via crystal structure in trans-(ClO4)2CuII(Me2N-Py)4 complex: S9/S6/C-H…O synthons, thermal, physicochemical and 1BNA-docking

The synthesis of the trans-(ClO4)2CuII(Me2N-Py)4 complex (where Me2N-Py = N,N-dimethylpyridin-4-amine ligand) in significant yield involved the reaction of excess Me2N-Py ligand with Cu(ClO4)2·6H2O in MeOH solvent. The progression of the complex synthesis was monitored by UV–Vis, FT-IR, EDX, XRD, MS, SEM, CHN-EA, and TG/DTG. The resulting complex crystallized in the Triclinic space P1¯. In the solid state, the desired complex structure discrete a neutral trans-(ClO4)2CuII(Me2N-Py)4 formula, with copper(II) adopting a distorted octahedral environment formed by four Me2N-Py ligands occupying the square planar position Cu(Py)4, meanwhile, the two ClO4 are in linear trans-position. The XRD measurements proved the existence of elongation Jahn-Teller distortion in the synthesized complex. Furthermore, a comprehensive analysis of the desired complex, including. XRD/HSA interactions revealed the presence of two S9 and S6 main 2D-synthons primarily formed through Cph-H….OClO3 and CMe-H…OClO3 non-classical H-bonding interactions. Thermal stability assessments through TG/DTG indicated that the complexes with the most stable behavior underwent two degradation steps. The molecular docking of the desired complex against 1BNA-DNA was also evaluated

Progesterone and Follicle Stimulating Hormone interact and promote goat preantral follicles survival and development in vitro

We investigated the effects of progesterone and follicle stimulating hormone (FSH) on survival and growth of caprine preantral follicles. Pieces of ovarian tissue were cultured for 1 or 7 days in minimum essential medium (MEM) alone or containing progesterone (1, 2.5, 5, 10 or 20ng/mL), FSH (50ng/mL) or the interaction between progesterone and FSH. Fresh (non-cultured control) and cultured ovarian tissues were processed for histological and ultrastructural studies. After 7 days the addition of FSH to all progesterone concentrations maintained the percentage of normal follicles similar to fresh control. At day 7 of culture, a higher percentage of developing follicles was observed only in 2.5ng/ml of progesterone associated with FSH or 10ng/ml of progesterone alone when compared with control. From day 1 to day 7 of culture, a significant increase in the percentage of developing follicles was observed in MEM and 2.5ng/ml of progesterone + FSH. In addition, after 7 days, in all treatments, there was a significant increase in follicular diameter when compared with control, except for MEM alone and in 5ng/ml of progesterone + FSH or 10ng/ml of progesterone alone. Ultrastructural studies confirmed follicular integrity after 7 days of culture in 2.5ng/ml of progesterone with FSH. In conclusion, this study demonstrated that the interaction between progesterone and FSH maintains ultrastructural integrity, stimulates primordial follicles activation and further growth of cultured caprine preantral follicles