Relationship between work - family conflict and marital satisfaction among nurses and midwives in hospitals of Zabol university of medical sciences

Background: Work-family conflicts described as incompatibility between work and family roles. There is mutual relationship between marital satisfaction and job so that the tension in one of two areas of career and family are affected. Objective: To examine the relationship between marital satisfaction and work-family conflict among nurses and midwives. Methods: All of 289 employees of married nursing and midwifery of Zabol University of Medical Sciences hospitals participated in the study in 2014. The data were collected with questionnaires of Enrich marital satisfaction and Carlson work-family conflict and were analyzed with statistical tests including Pearson correlation coefficient, t-test and linear regression analysis. Findings: Marital satisfaction score of the staff was 168.52 which indicates the relative satisfaction of spouses from each other. The mean score of work-family conflict among employees was 3.26; it can be said that employees in terms of work-family conflict, the conflict a moderate experience. There is a significant negative correlation among marital satisfaction and work-family conflict of employees. In fact, marital satisfaction decreases when the conflict between work and family is decreased. Nursing staffs have a higher marital satisfaction and in terms of work-family conflict they experience less conflict. Conclusion: According to the findings, the managers should create conditions that minimize the role conflicts and consequently increase the level of marital satisfaction. Keywords: Marital Satisfaction, Work-Family Conflict, Nurse, Midwif

Undersökning av sälar insamlade 2016 och 2017

Sedan 1970-talet har gråsäl (Halichoerus grypus), knubbsäl (Phoca vitulina) och vikare (Pusa hispida botnica) insamlats och undersökts på Naturhistoriska riksmuseet (NRM). Majoriteten av de undersökta sälarna är gråsälar. Beroende på jakt under 1900-talet och nedsatt reproduktion under århundradets senare del så minskade sälpopulationerna i Östersjön. Under 1970-och 1980-talet noterades ett antal sjukliga förändringar som kallades Baltic Seal Disease Complex framför allt hos gråsäl och som misstänktes ha samband med höga halter av miljögifter som PCB och DDT. Förekomsten av flera av dessa förändringar liksom halterna av PCB och DDT har minskat sedan dess. Hos gråsäl har därefter förekomst av tarmsår ökat och minskat, späcktjockleken minskat och förekomst av leverparasiter ökat. Under år 2016 och 2017 insamlades och undersöktes  sälar och prover från 188 gråsälar, 74 knubbsälar och 68 vikare. I övrigt inrapporterades 441 döda sälar funna på stränder av allmänheten och dödligheten verkade ligga på en normal nivå över året. Andelen gråsälshonor mellan 6-24 år gamla som var dräktiga var 88 % (2017). Dräktighetsfrekvensen under 2016 och 2017 var 70% för knubbsäl och 83% för vikare, men antalet honor i det åldersspannet var avsevärt få (10 respektive 12) vilket gör resultatet osäkert. De tre sälarterna har olika typer och grad av förändringar. De har exempelvis olika förekomst och skador av parasiter. Leverflundra och hakmask förekommer hos alla tre arter men infektionen är generellt värre och verkar göra mest skada hos gråsälen.Since the 1970s, grey seals (Halichoerus grypus), harbour seals (Phoca vitulina) and ringed seals (Pusa hispida bothnica) have been collected and necropsied at the Swedish Museum of Natural History. Mostly grey seals have been examined. The effect of hunting during the 19th century and reproductive failure during the latter half of the century decreased Baltic seal populations. In the 1970s and 1980s, pathological changes found mostly in grey seals was called the Baltic Seal Disease Complex and was thought to be related to high levels of PCB and DDT. Since then several pathological changes have decreased in prevalence as well as levels of PCB and DDT. Since then in grey seals, the prevalence of intestinal ulcers has increased and then decreased, blubber thickness has decreased and the prevalence of a zoonotic biliary trematode infection increased. In 2016/2017, whole bodies and samples from 188 grey seals, 74 harbour seals and 68 ringed seals were examined at the museum. The public reported 441 seals found dead along the Swedish coast and the mortality seemed to be at normal levels throughout the year. The percentage of pregnant grey seal females between 6 and 24 years old was 88% in 2017.  The proportion of examined pregnant harbour seals in corresponding age group was 70% and in ringed seals 83%, although the number of females was low (10 and 12 respectively) and therefore the confidence in the results is low. The three seal species have different types and severities of lesions. For example, parasites species differs and lesion related to parasites differs. Liver trematodes and acanthocephalan parasites in the colon occurs in all three species but the infection generally is larger and causes more lesions in the grey seal

A Full GMP Process to Select and Amplify Epitope-Specific T Lymphocytes for Adoptive Immunotherapy of Metastatic Melanoma

A number of trials of adoptive transfer of tumor-specific T lymphocytes have been performed in the last 20 years in metastatic melanoma, with increasingly encouraging results as the relevant melanoma antigens were identified and the purity/specificity of injected T cells improved. We have previously described a sorting method of epitope-specific T lymphocytes that uses magnetic beads coated with HLA/peptide complexes and we suggested that this method could be applied to a clinical setting. In the present work, we provide a detailed description of the whole GMP process of sorting and amplification of clinical grade T cells specific for the melanoma antigens Melan-A and MELOE-1. All the reagents used in this process including the sorting reagent were produced in GMP conditions and we document the optimization of the different steps of the process such as peptide stimulation, sorting, and amplification. The optimized procedure, validated in 3 blank runs in a clinical setting, allowed the production of at least 108 pure (>90%) Melan-A- and MELOE-1-specific T cells within 28 days starting with 100 mL of blood from metastatic melanoma patients. This GMP process is thus ready to be used in an upcoming phase I/II clinical trial on metastatic melanoma patients

Acne fulminans and Cutibacterium acnes phylotypes

International audienceBackground Acne fulminans (AF) is a rare and severe form of inflammatory acne. It is characterized by a sudden worsening of acne with appearance of ulceronecrotic lesions, which can be associated with systemic signs. Its pathophysiology and the best therapeutic strategy are only partially known. Objective Our main objectives were to describe the clinical and biological profile of AF patients and to determine whether there was a difference in Cutibacterium acnes phylotype in AF compared to acne vulgaris. The secondary objective was to assess the efficacy of different therapies. Methods A retrospective observational study was conducted in all patients followed for AF in our department between 2008 and 2018. Bacteriological samples were taken from each patient to analyse C. acnes phylotype distribution. The therapeutic response was assessed using the ECLA and GEA scales. Results Fifteen patients with a median age of 15 years were included (12 men, 80%). A family history of acne was found in 86.7% of patients. Nine patients (60%) had isotretinoin-induced AF. Only one patient (6.7%) showed systemic signs. The bacteriological culture was positive for C. acnes in 80% of patients. The predominant phylotype was IA1 in 60% of patients, corresponding to the predominant phylotype in acne vulgaris. Only 33.3% of patients were in remission after a first-line treatment with systemic corticosteroids, alone or in combination. Seven patients were treated with biotherapy, including five successfully with secukinumab. Conclusion Our results suggest that there is no specific C. acnes phylotype associated with AF, raising the hypothesis that acute inflammation associated with AF may be more related to an abnormal cutaneous innate immunity activation. The use of preventive strategies, the impact of combined treatments and an assessment of the role of biotherapies, especially anti-IL-17, in AF treatment remain to be more investigated

Binding studies of crocin to β-Lactoglobulin and its impacts on both components

© 2020 Elsevier Ltd Light susceptibility of crocin and high degree of unsaturation limit its application in food systems. This study aimed to investigate the impact of molecular binding of crocin to β-lactoglobulin (β-LG) on crocin stability as well as the conformational stability of β-LG. The overall binding affinity was estimated about 1.74 × 103 M−1 through following UV spectral changes of β-LG at different concentrations of crocin. A 1:1 binding stoichiometry for the complex of crocin and β-LG was recognized using Job's method. The results of molecular docking and molecular dynamic simulation indicated that in addition to a single hydrogen bond with an oxygen linker attached to polyene chain, hydrophobic interactions play the major role in binding of crocin's polyene chain to β-LG surface. Three-dimensional emission and CD spectra showed conformational alterations in both tertiary and secondary structure of β-LG varying with crocin concentration. Depending on the temperature, crocin showed a dual effect on the thermodynamic stability of β-LG. Crocin destabilized β-LG up to room temperature, but stabilized at higher temperatures. The CD spectra of crocin in the Soret region and simulation results proved the bending of polyene chain and changes in the angle between sugar groups and polyene chain in combination with the protein. β-LG complexation enhanced the chemical stability of crocin by 20% during a 30-day storage, while its degradation was accelerated under UV irradiation. According to the results, it is predicted crocin functionalities to be affected by β-LG association and vice versa

Predictive value of FDG‐PET imaging for relapse in metastatic melanoma patients treated with immunotherapy

International audienceBackground: Anti-PD1 immunotherapy has shown a sustainable clinical activity in patients with metastatic melanoma. However, strong predictive factors of the long-term response or risk of relapse remain to be identified.Objectives: To determine whether FDG-PET imaging could be superior to CT scan in distinguishing residual tumours versus the absence of tumour in patients with a partial response (PR) or stable disease (SD) and whether a complete metabolic response (CMR) was associated with better outcomes.Methods: Retrospective study conducted in all patients with metastatic melanoma treated with anti-PD1 immunotherapy between October 2014 and October 2017 considered to be in complete remission. The primary outcome was the occurrence of a relapse during the follow-up. CT scan and FDG-PET scan had to be performed within a maximum of 2 months of treatment discontinuation. For CT imaging, the Response Evaluation Criteria in Solid Tumours (RECIST) 1.1 were used and included progressive disease (PD), SD, PR and complete response (CR). For FDG-PET imaging, the metabolic responses were classified as progressive metabolic disease, stable metabolic disease, residual FDG avidity (RFA) and CMR.Results: Twenty-six patients were in complete remission after collegial decision. Two patients had a SD on CT scan and a CMR on FDG-PET scan, and none of them relapsed. Ten patients had a PR on CT scan and a CMR on FDG-PET scan, and none of them relapsed. The mean treatment duration to achieve a complete remission was 7 months (3-23). A univariate analysis showed that a RFA assessed on the FDG-PET scan was significantly associated with a relapse (P = 0.00231).Conclusions: Most patients with a PR on the CT scan and a CMR on the FDG-PET scan should be considered with a CR. Our study showed that FDG-PET imaging could play a crucial role in predicting the long-term outcome and help to decide whether treatment should be discontinued