Phosphatidylinositol-specific phospholipase C interaction with G-proteins in relation to the environmental stress response in wheat (Triticum aestivum)

Tolerance to environmental stress in plants is controlled by several internal factors and environmental signals. In this study, we have focused on phosphatidylinositol-specific phospholipase C (TaPI-PLC) and phosphoglycerol-specific phospholipase C (TaPG-PLC) in wheat, Triticum aestivum . Three homologs of TaPG-PLCs were identified as cDNA with high sequence similarity to rice and Arabidopsis PG-PLC1. TaPG-PLC3 was mapped on the distal part of the long arm of chromosome 5A, whereas TaPG-PLC1 and TaPG-PLC2 like genes appear to be duplicated in two of hexploid wheat 3 three genomes. A TaPG-PLC1::Green fluorescent protein (GFP)-fusion was localized on endoplasmic reticulum (ER), golgi and plasma membrane (PM). Important protein-protein interactions were found for wheat PI-PLCs. The PI-PLCI::GFP fusion was localized on ER-and PM. In vivo interactions between TaPI-PLC1 and two classes of G-proteins, the heterotrimeric G protein subunit TaGa and a non-canonicat G-protein J822, were detected using Bimolecular Fluorescent Complementation (BiFC). The interactions were detected in both the ER and PM. In contrast, TaPI-PLC2::GFP fusion was localized only on PM and was not found to interact with TaGÜ or J822 . Since both TaPI-PLC 1 and J822 are up-regulated by cold stress and it is possible that they play an important role in signaling during cold acclimatio

The characterization of the caleosin gene family in Triticeae and their role in G-protein signalling and Identification and characterization of rye genes silenced in allohexaploid triticale: A bioinformatic study

ABSTRACT The characterization of the caleosin gene family in Triticeae and their role in G-protein signalling and Identification and characterization of rye genes silenced in allohexaploid triticale: A bioinformatic study Hala Badr Abdel-Sadek Khalil, Ph.D. Concordia University, 2013 The caleosin genes encode proteins with a single conserved EF hand calcium-binding domain and comprise small gene families found in a wide range of plant species. In this study, Clo3, a member of caleosin family in hexaploid wheat (Triticum aestivum), has been shown to play an important role in signaling by both in vivo and in vitro analyses of its interaction with Gα, the alpha subunit of heterotrimetric GTP binding protein. This interaction increased the GTPase activity of Gα by approximately 25%. Eleven paralogous groups of caleosins, which comprise a total of thirty-four caleosin genes, have been assembled and identified using the T. aestivum GenBank EST database and ten gene family members were assembled from Secale cereale 454-cDNA sequences. The analysis of caleosin gene expression was assayed by RNA-Seq analysis of 454 sequence sets and members of the gene family were found to have diverse patterns of gene expression in the nine tissues that were sampled in rye and in triticale, a synthetic polyploid species derived from durum wheat (Triticum turgidum) and rye (Secale cereale). The impact of the polyploidization event on rye genes in the triticale background was investigated using both caleosin genes and whole transcriptome comparisons. The high-throughput cDNA sequence comparison between the diploid rye and the hexaploid triticale detected suppression of expression of approximately 2% of the rye genes surveyed in the triticale. The expression of 23503 rye cDNA contigs was analyzed in 454-cDNA libraries obtained from anther, root and stem from both triticale and rye as well as in five 454-cDNA data sets created from ovary, pollen, seed, seedling shoot and stigma from triticale. Among these, 112 rye cDNA contigs were found to be totally suppressed in all triticale tissues, although their expression was relatively high in rye tissues. Suppressed rye genes were found to have strikingly low similarity to their closest BASTN matches in a current draft of the wheat genome available through the International Wheat Genome Survey Consortium, IWGSC. The comparison of rye silenced genes to wheat database revealed that 89% of rye genes silenced in triticale do not have a best match in T. aestivum with sequence identity higher than 90%, whereas 59% of random rye contigs had a best hit of 90% or higher in T. aestivum. The comparisons to the draft genomes of Triticum. urartu, and Aegilops. tausshii, the A and D genome donors to T. aestivum, respectively, support the previous observation. PCR assays found that 6 out of 10 candidate suppressed genes were deleted from the triticale genome

Effect of Educational Program on Outcomes of Patients Undergoing Permanent Pacemakers’ Implantation

Contents: Patients undergoing permanent implantable pacemaker, challenge with multiple physical, psychological along with social complications. Teaching patients the essential points involving pacemaker management can prevent complications.Aim: The current study aimed to evaluate the effect of an educational program on outcomes of patients undergoing permanent pacemakers' implantation. Methods: A quasi-experimental pre/posttest and follow-up design were used to conduct this study. A convenient sample of 35 adult patients from both genders undergoing permanent pacemaker implantation was recruited from Beni-Suef University hospital's catheter lab, inpatient department, ICU, CCU, and outpatient cardiology clinic. Patients' interviewing questionnaire, patient performance checklist, pacemaker self-efficacy scale, and Aga Khan University Anxiety and Depression Scale were used to achieve this study's aim. Results: 91.4% of the studied patients had unsatisfactory total knowledge at the baseline, improved to 85.7% had a satisfactory knowledge immediately post educational intervention, and relatively maintained to 68.6% of them had a satisfactory level of total knowledge after four weeks of the program implementation. 97.1% of the studied patients had unsatisfactory total practice at the baseline, improved to 65.7% had a satisfactory practice immediately post educational intervention, and improved to 77.1% of them had a satisfactory level of total practice after four weeks of program implementation. 71.4%, and 85.7% of the studied patients were not confident at all with their ability to control symptoms and maintain their usual functions respectively before education. 91.4% of them reported a total low self-efficacy score before the educational intervention. In comparison, 45.7% were very confident in controlling symptoms and moderately confident in maintaining usual functions at the follow-up phase. Besides, 65.7 exhibited total high self-efficacy at the follow-up assessment. 57.1% of the studied patients exhibit severe anxiety at the baseline assessment, while 65.7% and 74.3% exhibit mild anxiety at the post and follow-up assessment. Conclusion: A statistically significant improvement in patients' knowledge, practice, self-efficacy, anxiety, and depression immediately and after four weeks compared to their baseline. The study recommended that the educational program be an essential part of the total management of patients undergoing implanted pacemakers

Electronic conductance of quantum wire with serial periodic potential structures

A theory based on the total transfer matrix is presented to investigate the electronic conductance in a quantum wire with serial periodic potentials. We apply the formalism in computation of the electronic conductance in a wire with different physical parameters of the wire structure. The numerical results could be used in designing some future quantum electronic devices

Bone regeneration using deproteinized bovine bone mineral (DBBM) in a crestal sinus elevation technique: a histological and histomorphometrical pilot study

The aim of this study is to evaluate the percentage of new bone formation (NBF) and residual bone substitute following the use of a bovine demineralized xenograft (DBBM) in a sinus floor crestal approach elevation technique

Synthesis and antitumor activity of novel pyrazolo[1,5-a]pyrimidine derivatives

A novel series of pyrazolo[1,5-a]pyrimidine-3-carbonitriles substituted with 7-amino, 7-substituted amino and 5-substituted amino groups was synthesized. Some of the newly synthesized compounds were tested in vitro on human colon tumor cell line (HCT116). Compound 14a displayed the highest activity among the tested compounds with IC50 that equals to 0.0020 μM

Child‐level double burden of malnutrition in the MENA and LAC regions: Prevalence and social determinants

Although the prevalence of obesity has rapidly increased in the low‐ and middle‐income countries of the Middle East and North Africa (MENA) and Latin America and the Caribbean (LAC) regions, child undernutrition remains a public‐health challenge. We examined region‐specific sociodemographic determinants of this double burden of malnutrition, specifically, the co‐occurrence of child stunting and overweight, using Demographic and Health Survey and Multiple Indicator Cluster Survey data (2003–2016) from 11 countries in the MENA (n = 118,585) and 13 countries in the LAC (n = 77,824) regions. We used multiple logistic regressions to model region‐specific associations of maternal education and household wealth with child nutritional outcomes (6–59 months). The prevalence of stunting, overweight, and their co‐occurrence was 24%, 10%, and 4.3% in children in the MENA region, respectively, and 19%, 5%, and 0.5% in children in the LAC region, respectively. In both regions, higher maternal education and household wealth were significantly associated with lower odds of stunting and higher odds of overweight. As compared with the poorest wealth quintiles, decreased odds of co‐occurring stunting and overweight were observed among children from the second, third, and fourth wealth quintiles in the LAC region. In the MENA region, this association was only statistically significant for the second wealth quintile. In both regions, double burden was not statistically significantly associated with maternal education. The social patterning of co‐occurring stunting and overweight in children varied across the two regions, indicating potential differences in the underlying aetiology of the double burden across regions and stages of the nutrition transition.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/154671/1/mcn12923_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/154671/2/mcn12923.pd

Toxic pathological effect of mitomycin-c on male reproductive organs in rat

The aim of the present study has detected the effect of Mitomycin-c on the histological structure on a male reproductive organ. In this study used (24) healthy adult male rats were divided into three groups, each group includes (8) animals: the 1st toxic group was injected (6.5mg/kg) of Mitomycin-c subcutaneous (s/c) once daily, the 2ed therapeutic or low dose group was injected (3.25 mg/kg) of Mitomycin-c subcutaneous (s/c) once daily, while the 3rd control group was injected physiological normal saline (0.9% NaCl2). The experimental period was (10) weeks. Animal, which was treated with Mitomycin-c along the period of the experiment showed, severe pathological changes, particularly in testes and epididymis

Pathological effects of mercury chloride on reproductive system in white rats

The present study was undertaken to know the reproductive toxicity of mercury chloride in male and females of white rats. This study was used twelve (6 male and 6 females) white rats of approximately of the same body weight (200-220 g) divided equally in to 3 groups; the first group (T1) was received mercury chloride (1mg/kg B.W intraperitoneally once daily for 30 days). While the second group (T2) was received, mercury chloride (1.5mg/kg B.W intraperitoneally once daily for 30 days).Third group was received only0.2ml of Distilled water considered as control group. At the end of an experiment, the animals were sacrificed and small pieces of (2cm³) were taken from ovary, uterus and testis of all animals to histopathology. Histopathological sections of these organs of (T2) group was showed severe pathological changes characterized by vaculation in epithelial cells of uterus, cystic dilatation of uterine glands with degeneration of epithelial lining of its ,hemorrhage and decrease in number of growing follicles in ovary also there were sever pathological changes in the testes. While (T1) group was showed less pathological changes characterized by hyperplasia of epithelial lining with few and small uterine gland in uterus, presence of large secondary follicles in ovary and there were less pathological changes in testes