118 research outputs found
Comparison of the specific activity of ribuloseâ1,5âbisâphosphate carboxylaseâoxygenase from some C3 and C4 plants
The specific activity of ribuloseâ1,5âbisphosphate carboxylaseâoxygenase (Rubisco, EC 4.1.1.39) was measured from the crude extracts of five C3 plants consisting of wheat (Triticum aestivum L. cv. Maris Mink), spinach (Spinacia oleracea L.), pea (Pisum sativum L. cv. Greenfeast), pumpkin (Cucurbita pepo L. cv. JĂ€ttilĂ€ismeloni) and Ceratodon purpureus (Hedw.) Brid., and two C4 plants, maize (Zea mays L. ETA F1) and sugar sorghum [Sorghum saccharatum (L. emend, L.) Moench]. The amount of Rubisco in the crude extracts was estimated by polyacrylamide gel electroâphoresis with the Coomassie Brilliant Blue staining procedure. The amounts of the dye bound to the purified Rubisco of different higher plants were similar. The method gave a linear response for both purified enzyme and crude extracts, and the results agreed with those observed by immunochemical methods. The addition of positive effectors such as inorganic phosphate was necessary to obtain maximal activity in the crude extracts of all the studied plants except in that of maize. No significant differences in the specific carboxylase activity at 25°C were found between the C3 and C4 plants
Elucidating the biosynthesis of 2-carboxyarabinitol 1-phosphate through reduced expression of chloroplastic fructose 1,6-bisphosphate phosphatase and radiotracer studies with <sup>1</sup><sup>4</sup>CO<sub>2</sub>
A point mutation in the N-terminus of ribulose-1,5-bisphosphate carboxylase affects ribulose-1,5-bisphosphate binding
Mutations in loop six of the large subunit of ribulose-1,5-bisphosphate carboxylase affect substrate specificity
The Effects of Co2 Enrichment on the Biochemistry of Photosynthesis and Photorespiration of Spruce Trees Cultivated in Open-Top Chambers.
Bacterial superinfection with venereal pathogens subsequent to topical therapy for Taylorella equigenitalis in stallions
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