The Cowl - v.33 - n.12 - Dec 10, 1980

The Cowl - student newspaper of Providence College. Vol 33 - No. 12 - December 10, 1980. 16 pages

Isolation of Indigenous Bacteria from Paddy Field for Methomyl Degradation

Methomyl is an active ingredient of carbamate group pesticide. The uncontrolled application of methomyl may contaminate the water and soil. The objective of this research was to find indigenous bacteria that could degrade the methomyl. The soil samples were taken from the soil of the rice field located in Musi Rawas District, South Sumatera, Indonesia. The soil bacteria that were found to degrade methomyl were isolated by using a medium containing methomyl. There were 2 of 16 isolates that could grow in a high concentration of methomyl and they were Acinetobacter baumannii and Bacillus megaterium

ROMK expression remains unaltered in a mouse model of familial hyperkalemic hypertension caused by the CUL3Δ403‐459mutation

Familial hyperkalemic hypertension (FHHt) is a rare inherited form of salt‐dependent hypertension caused by mutations in proteins that regulate the renal Na+‐Cl‐ cotransporter NCC. Mutations in four genes have been reported to cause FHHt including CUL3 (Cullin3) that encodes a component of a RING E3 ligase. Cullin‐3 binds to WNK kinase‐bound KLHL3 (the substrate recognition subunit of the ubiquitin ligase complex) to promote ubiquitination and proteasomal degradation of WNK kinases. Deletion of exon 9 from CUL3 (affecting residues 403‐459, CUL3Δ403‐459) causes a severe form of FHHt (PHA2E) that is recapitulated closely in a knock‐in mouse model. The loss of functionality of CUL3Δ403‐459 and secondary accumulation of WNK kinases causes substantial NCC activation. This accounts for the hypertension in FHHt but the origin of the hyperkalemia is less clear. Hence, we explored the impact of CUL3Δ403‐459 on expression of the distal secretory K channel, ROMK, both in vitro and in vivo. We found that expressing wild‐type but not the CUL3Δ403‐459 mutant form of CUL3 prevented the suppression of ROMK currents by WNK4 expressed in Xenopus oocytes. The mutant CUL3 protein was also unable to affect ROMK‐EGFP protein expression at the surface of mouse M‐1 cortical collecting duct (CCD) cells. The effects of CUL3 on ROMK expression in both oocytes and M‐1 CCD cells was reduced by addition of the neddylation inhibitor, MLN4924. This confirms that neddylation is important for CUL3 activity. Nevertheless, in our knock‐in mouse model expressing CUL3Δ403‐459 we could not show any alteration in ROMK expression by either western blotting whole kidney lysates or confocal microscopy of kidney sections. This suggests that the hyperkalemia in our knock‐in mouse and human PHA2E subjects with the CUL3Δ403‐459 mutation is not caused by reduced ROMK expression in the distal nephron

Technological expectations and global politics:Three waves of enthusiasm in non-governmental remote sensing

Media, industry and academia frequently depict the commercialization of satellite imagery as geospatial revolution with transformational effects on global politics. In doing so, they follow an understanding that isolates technology from politics. While this division is still prevalent in International Relations, recent scholarship has promoted the intricate relationship of technology with politics as socio-material. Adding to this literature, I draw on the sociology of expectations to propose an alternative reading of non-governmental remote sensing. For this purpose, the notion of techno-political barriers is introduced to trace controversies about technological expectations of satellite imagery. Based on expert interviews and document analysis, I identify three waves of enthusiasm, which are characterized by particularly salient expectations and techno-political barriers. The first wave is fuelled by an enthusiasm about the general benefits of visual transparency as opposed to Cold War secrecy. The second wave turns towards non-governmental imagery intelligence for human security. In the third wave satellite imagery joins multiple data streams to support political and business decisions. Taken together, the three-waves model distorts the linear understanding of a revolutionary development but reveals the political and controversial nature of the ongoing commercialization of satellite imagery. As a part of this, non-governmental remote sensing has experienced a focus shift from visual transparency towards geospatial big data. Moreover, the three waves model highlights the persistence of expectations and techno-political barriers in the non-governmental sector with important implications for policymaking and the global impact of commercial satellite imagery

The Montclarion, March 22, 2007

Student Newspaper of Montclair State Universityhttps://digitalcommons.montclair.edu/montclarion/2231/thumbnail.jp

Black, White, Brown, Green, and Fordice: The Flavor of Higher Education in Louisiana and Mississippi

Black, White, Brown, Green, and Fordice: The Flavor of Higher Education in Louisiana and Mississippi chronicles the higher education desegregation sagas in Louisiana and Mississippi. The Article specifically compares the histories of the higher education desegregation lawsuits in the two states and their subsequent experiences and progress under Settlement Agreements. The statistical populations of many universities in both states are still largely identifiable as “white” or “black,” and so the Article will pose questions not only respecting the implementation of United States v. Fordice in both states, but also respecting the value, desirability, or possibility of the “integrative ideal” converting “black schools” and “white schools” to “just schools.