Purification and characterization of two human liver carboxylesterases

1. 1. Two carboxylesterases (EC 3.1.1.1) purified from human livers were distinguished by pi (isoelectric point), nondenaturing polyacrylamide gel electrophoresis, molecular weight, catalytic activity, N-tenninus and immunological cross-reactivity. 2. 2. The low pI carboxylesterase has not been reported previously. 3. 3. Numerous bands seen when each enzyme was focused on analytical IEF gels could not be separated. 4. 4. When sections of the band pattern was refocused, the original complete band pattern was generated. 5. 5. Both the mid and low pI carboxylesterases had catalytic activity for xenobiotics as well as medium and long chain fatty acid esters

Epsilon glutathione transferases possess a unique class-conserved subunit interface motif that directly interacts with glutathione in the active site

10.1042/BSR20150183Bioscience Reports356e0027