22 research outputs found
Freeze-dried, mucoadhesive system for vaginal delivery of the HIV microbicide, dapivirine: optimisation by an artificial neural network
The relevance of the amorphous state to pharmaceutical dosage forms: glassy drugs and freeze dried systems
Investigation of the Binding of Tween 20 and Tween 80 to Lactate Dehydrogenase Using ITC
An investigation into the mechanism of cryoprotection of lactate dehydrogenase by non-ionic surfactants using interfacial rheology
An Investigation of the Cryoprotection of Lactate Dehydrogenase by Non-ionic Surfactants
Temperature controlled Raman microscopy for the imaging of crystallisation and polymorphic transitions in frozen systems
Development of polymeric–cationic peptide composite nanoparticles, a nanoparticle-in-nanoparticle system for controlled gene delivery
We report the formulation of novel composite nanoparticles that combine the high transfection efficiency of cationic peptide-DNA nanoparticles with the biocompatibility and prolonged delivery of polylactic acid–polyethylene glycol (PLA-PEG). The cationic cell-penetrating peptide RALA was used to condense DNA into nanoparticles that were encapsulated within a range of PLA-PEG copolymers. The composite nanoparticles produced exhibited excellent physicochemical properties including size 80%. Images of the composite nanoparticles obtained with a new transmission electron microscopy staining method revealed the peptide-DNA nanoparticles within the PLA-PEG matrix. Varying the copolymers modulated the DNA release rate >6 weeks in vitro. The best formulation was selected and was able to transfect cells while maintaining viability. The effect of transferrin-appended composite nanoparticles was also studied. Thus, we have demonstrated the manufacture of composite nanoparticles for the controlled delivery of DNA