Antibiotic prescribing pattern among paediatric patients attending tertiary care hospital in South India

Background: The usage of antibiotics among paediatric age group in India is on the higher side, that more than 60% of children in the age group 0 to 4 years received antibiotics. The higher use of antibiotics results in antibiotic resistance, increased health care costs, adverse drug reaction and may complicate the treatment of infections in future. There is a continuous need to monitor the prescription of antibiotics at all health care levels to prevent antibiotic resistance.Methods: A prospective and descriptive study was conducted in one of the pharmacies which dispenses the paediatric prescriptions in a tertiary hospital. The prescriptions used to treat infection were collected and analysed. A total of 500 prescriptions were collected and analysed.Results: The prescriptions were analysed for the WHO prescribing indicators. The average number of drugs per prescription is 1.84 with 21% of the prescriptions containing antibiotics. The most commonly prescribed antibiotics were penicillin like amoxycillin, phenoxymethyl penicillin followed by cotrimoxazole and cephalosporin group of antibiotics. The antibiotics prescribed belonged to the access group of antibiotics. The percentage of the prescriptions with drugs form essential drugs list and prescribed with generic name is 65.8% and 67% respectively. There were no injections prescribed.Conclusions: The antibiotic prescribing pattern and the average drugs per prescription falls with the WHO range indicating reduced use of antibiotics and absence of polypharmacy. However, the prescription of generic drugs and the drugs from the essential drug list is less

Antifungal activity of ethanolic extract of Sphaeranthus indicus Linn. against Dermatophytes and Candida species

Background: Sphaeranthus indicus as an entire plant or the specific parts like roots, leaves, flowers are used for treating helminthiasis, jaundice, diabetes, dyspepsia, fever, cough, hernia, gastritis, hemorrhoids, migraine, leprosy and skin diseases. The objective of this study was to evaluate the antifungal activity of ethanolic extract of the whole plant Sphaeranthus indicus Linn. Methods: The antifungal activity of ethanolic extract of the whole plant Sphaeranthus indicus Linn was evaluated by incorporating the extract in Sabourauds dextrose agar. This property was studied in vitro using the ethanolic extract on Candida albicans, Microsporum gypseum, Trichophyton mentagrophytes and Epidermophyton floccosum by using Clotrimazole as standard drug.Results: This study revealed that the ethanolic extract of Sphaeranthus indicus is having good antifungal activity against Dermatophytes and Candida species. Conclusions: This study demonstrates the promising antifungal action of ethanolic extract of whole plant of Sphaeranthus indicus Linn. This feature can be exploited in the development of a newer antifungal agent from plant.

COMPUTATIONAL FLUID DYNAMIC ANALYSIS OF VARIABLE INLET AREA RAMJET ENGINE

The usage of ramjet engine is very important in the missile and aircraft propulsion. When it is cruising at supersonic air velocity the flow characteristic of the ramjet engine produces a shock wave. This formation of shock wave will raise the pressure of the upstream airflow in front of the inlet cone. This pressure rise with reduced air speed is suitable for the subsonic combustion of the ramjet engine. The cone will help to change the area of the ramjet engine inlet and mass flow rate of the incoming pressure raised air. The change in mass flow rate of the inlet leads to the variation in thrust production. This engine is integrated with a convergent-divergent nozzle to produce supersonic velocity at the nozzle exit. This variation in thrust production is to be studied in detail. In this project, the variable inlet area ramjet engine design and model is investigated. In order to predict the thrust value for variable inlet area ramjet engine by using commercial numerical codes. The increment of thrust value will be expected by using the variable inlet area ramjet engine, which means it attains more thrust

In vitro evaluation of antibacterial activity in ethanolic extract of whole plant Sphaeranthus indicus Linn.

Background: Sphaeranthus indicus Linn is a widely used medicinal plant in Indian traditional system of medicine against human pathogens. Alarming bacterial resistance is urging scientist to search for newer anti-microbial substances from the medicinal plants. The objective of the study was to evaluate the antibacterial activity of ethanolic extract of the whole plant Sphaeranthus indicus Linn (Asteraceae).Methods: The antibacterial activity of ethanolic extract of whole plant of Sphaeranthus indicus Linn was done against Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis and Staphylococcus aureus in Mueller Hinton Agar (MHA) and compared with ciprofloxacin as standard by disc diffusion method.Results: The study revealed that there was no zone of inhibition in doses of 100 mcg, 200 mcg and 300 mcg of ethanolic whole plant extract of Sphaeranthus indicus in MHA plates compared with ciprofloxacin 30 mcg.Conclusions: Ethanolic extract of Sphaeranthus indicus does not have antibacterial activity. Further studies are needed in different extracts and parts of the plant. Simultaneous studies can be done in different places to evaluate environmental factors and regional variations

Maternal repair system- an analysis in retrospect

Influence of caffeine, cysteine, hydroxyures and cycloherimide on maternal repair system operative in female Drosophila was studied using rod-X males and Oster females. The number of parental matings was kept constant. A new method of statistical analysis was employed to examine the frequency of sex-chromosome loss in these experiments. A re-analysis of published data was also done using this method. The existence of a maternal repair system was not upheld. The observed discrepancies between data reported herein and those published earlier and the possible reasons are discussed

Quantitative ecology of phytoplankton in the Cochin backwater

A one-year study on the standing crop in terms of chlorophylls, primary production and total-cell counts of four station grids has shown that there were regional and seasonal variations in the magnitude of phytoplankton production in the Cochin backwater. Statistically treated using a microcomputor, a multiple regression relationship has been established between parameters within stations. But th<^ Correlation coefBclent and Standard Regression coefficient have revealed that the plankton production and the related parameters at all the stations were independent of each other and the parameters varied from station to station. Probable reasons are briefly discussed

Observations on the wastage of raw material and recovery of meat in the prawn processing industry

The wastage of prawns due to spoilage in processing factories accounted to about 0-12% in 1974, 0-35% in 1975, 0-3% in 1976 and 0-4% in 1977. Spoilage increases with the time lag between catching and processing and also due to defective icing. The paper discusses the counts of whole prawns required for obtaining meat of specified size grades

Hsp90 inhibition: A promising therapeutic approach for arsacs

Autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS) is a neurodegenera-tive disease caused by mutations in the SACS gene, encoding the 520 kDa modular protein sacsin, which comprises multiple functional sequence domains that suggest a role either as a scaffold in protein folding or in proteostasis. Cells from patients with ARSACS display a distinct phenotype including altered organisation of the intermediate filament cytoskeleton and a hyperfused mitochon-drial network where mitochondrial respiration is compromised. Here, we used vimentin bundling as a biomarker of sacsin function to test the therapeutic potential of Hsp90 inhibition with the C-terminal-domain-targeted compound KU-32, which has demonstrated mitochondrial activity. This study shows that ARSACS patient cells have significantly increased vimentin bundling compared to control, and this was also present in ARSACS carriers despite them being asymptomatic. We found that KU-32 treatment significantly reduced vimentin bundling in carrier and patient cells. We also found that cells from patients with ARSACS were unable to maintain mitochondrial membrane potential upon challenge with mitotoxins, and that the electron transport chain function was restored upon KU-32 treatment. Our preliminary findings presented here suggest that targeting the heat-shock response by Hsp90 inhibition alleviates vimentin bundling and may represent a promising area for the development of therapeutics for ARSACS

Knowledge, attitude and practices of antibiotic usage and resistance among the second year MBBS Students

Background: Social aspect of antibiotic management forms a significant way to overcome the rapidly intensifying problem of antibiotic resistance. Knowledge helps in spreading awareness and understanding while attitude and practices influences health related behaviour. Medical students should not only be made aware of the current emerging health issues but also be directed towards rational antibiotics prescribing behaviour as future medical practitioners. Therefore, present study was undertaken to assess the existing knowledge, attitude and practices (KAP) related to antibiotic usage and resistance among second year (fourth semester) MBBS students.Methods: This was a cross-sectional, questionnaire based study conducted in department of pharmacology of a government medical college in Kerala, India. Questionnaire was distributed among a batch of 120 second year MBBS students after one of the pharmacology lecture session. Attitude and Practices related questions were assessed using Likert scale. Data was entered in excel and analysed using descriptive statistics of SPSS to generate frequency and percentage.Results: 91.6% (110) were considered valid for analysis. Mean age was 21±1 years. Students had fair knowledge regarding antibiotics usage and resistance with mean correct response 79.72±25.37%. Mean correct responses of attitude and practices related questions were found to be 55.95±25.63% and 64±13.79% respectively.Conclusions: Most of the students were aware of the antibiotic resistance and its consequences. However, their attitude and practices were found to be a matter of great concern. An educational intervention can be introduced to bring about behaviour changes regarding rational antibiotics prescribing among them